Identification of an Efs isoform that lacks the SH3 domain and chromosomal mapping of human Efs
Efs was originally found by expression cloning of a mouse embryo cDNA library through its Fyn-SH3 binding capacity (Ishino et al., Oncogene 11, 2331-2338, 1995). Efs has characteristic regions important in intracellular signal transduction; these are an SH3 domain, a cluster of putative ligands for...
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| Veröffentlicht in: | Oncogene 1997-10, Vol.15 (14), p.1741-1745 |
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| creator | ISHINO, M OHBA, T INAZAWA, J SASAKI, H ARIYAMA, Y SASAKI, T |
| description | Efs was originally found by expression cloning of a mouse embryo cDNA library through its Fyn-SH3 binding capacity (Ishino et al., Oncogene 11, 2331-2338, 1995). Efs has characteristic regions important in intracellular signal transduction; these are an SH3 domain, a cluster of putative ligands for SH2 domains and proline-rich sequences with SH3-binding consensus. In this paper, we report cDNA cloning of human Efs and a variant of it from a hippocampal cDNA library. The human Efs gene was mapped to chromosome 14q11.2-q12 by fluorescence in situ hybridization. We identified two forms of human Efs, designated hEfs1 and hEfs2. hEfs1 represents the human counterpart of original mouse embryo Efs (mEfs1). hEfs2, the newly identified form, is identical to hEfs1, except for its lack of the SH3 domain. hEfs1 and mEfs1 are 80% identical in their amino acid sequences and 100% identical within the SH3 domain. Reverse transcription polymerase chain reaction analysis of adult mouse tissue RNA indicated expression of Efs2 and of Efs1 in various tissues. Evidence suggesting the presence of the Efs2 protein in human tissue was obtained by immunoprecipitation followed by immunoblotting with two different anti-Efs antibodies. Possible functions of Efs2 are discussed. |
| doi_str_mv | 10.1038/sj.onc.1201346 |
| format | Article |
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Efs has characteristic regions important in intracellular signal transduction; these are an SH3 domain, a cluster of putative ligands for SH2 domains and proline-rich sequences with SH3-binding consensus. In this paper, we report cDNA cloning of human Efs and a variant of it from a hippocampal cDNA library. The human Efs gene was mapped to chromosome 14q11.2-q12 by fluorescence in situ hybridization. We identified two forms of human Efs, designated hEfs1 and hEfs2. hEfs1 represents the human counterpart of original mouse embryo Efs (mEfs1). hEfs2, the newly identified form, is identical to hEfs1, except for its lack of the SH3 domain. hEfs1 and mEfs1 are 80% identical in their amino acid sequences and 100% identical within the SH3 domain. Reverse transcription polymerase chain reaction analysis of adult mouse tissue RNA indicated expression of Efs2 and of Efs1 in various tissues. Evidence suggesting the presence of the Efs2 protein in human tissue was obtained by immunoprecipitation followed by immunoblotting with two different anti-Efs antibodies. Possible functions of Efs2 are discussed.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/sj.onc.1201346</identifier><identifier>PMID: 9349509</identifier><language>eng</language><publisher>Basingstoke: Nature Publishing</publisher><subject>Adaptor Proteins, Signal Transducing ; Alternative Splicing ; Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Chromosome 14 ; Chromosome Mapping ; Chromosomes, Human, Pair 14 ; Cloning ; Complementary DNA ; Fluorescence in situ hybridization ; Fundamental and applied biological sciences. Psychology ; Fyn protein ; Gene mapping ; Hippocampus ; Humans ; Immunoblotting ; Immunoprecipitation ; Mice ; Molecular and cellular biology ; Molecular Sequence Data ; Phosphoproteins - genetics ; Polymerase chain reaction ; Proline ; Protein Binding ; Reverse transcription ; RNA, Messenger - genetics ; Sequence Alignment ; Sequence Deletion ; Signal transduction ; src Homology Domains</subject><ispartof>Oncogene, 1997-10, Vol.15 (14), p.1741-1745</ispartof><rights>1997 INIST-CNRS</rights><rights>Macmillan Publishers Limited 1997.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-676876dc367ae81e79923cff58e9ec15fdc338e2bf94de8ae9ec974654830a323</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2844629$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9349509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ISHINO, M</creatorcontrib><creatorcontrib>OHBA, T</creatorcontrib><creatorcontrib>INAZAWA, J</creatorcontrib><creatorcontrib>SASAKI, H</creatorcontrib><creatorcontrib>ARIYAMA, Y</creatorcontrib><creatorcontrib>SASAKI, T</creatorcontrib><title>Identification of an Efs isoform that lacks the SH3 domain and chromosomal mapping of human Efs</title><title>Oncogene</title><addtitle>Oncogene</addtitle><description>Efs was originally found by expression cloning of a mouse embryo cDNA library through its Fyn-SH3 binding capacity (Ishino et al., Oncogene 11, 2331-2338, 1995). Efs has characteristic regions important in intracellular signal transduction; these are an SH3 domain, a cluster of putative ligands for SH2 domains and proline-rich sequences with SH3-binding consensus. In this paper, we report cDNA cloning of human Efs and a variant of it from a hippocampal cDNA library. The human Efs gene was mapped to chromosome 14q11.2-q12 by fluorescence in situ hybridization. We identified two forms of human Efs, designated hEfs1 and hEfs2. hEfs1 represents the human counterpart of original mouse embryo Efs (mEfs1). hEfs2, the newly identified form, is identical to hEfs1, except for its lack of the SH3 domain. hEfs1 and mEfs1 are 80% identical in their amino acid sequences and 100% identical within the SH3 domain. Reverse transcription polymerase chain reaction analysis of adult mouse tissue RNA indicated expression of Efs2 and of Efs1 in various tissues. Evidence suggesting the presence of the Efs2 protein in human tissue was obtained by immunoprecipitation followed by immunoblotting with two different anti-Efs antibodies. Possible functions of Efs2 are discussed.</description><subject>Adaptor Proteins, Signal Transducing</subject><subject>Alternative Splicing</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Chromosome 14</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 14</subject><subject>Cloning</subject><subject>Complementary DNA</subject><subject>Fluorescence in situ hybridization</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fyn protein</subject><subject>Gene mapping</subject><subject>Hippocampus</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Immunoprecipitation</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Phosphoproteins - genetics</subject><subject>Polymerase chain reaction</subject><subject>Proline</subject><subject>Protein Binding</subject><subject>Reverse transcription</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Deletion</subject><subject>Signal transduction</subject><subject>src Homology Domains</subject><issn>0950-9232</issn><issn>1476-5594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtv2zAUhYkgReo81mwFCKTIJpUv8TEWhtsYCNCh7UzQFBnTkUiXlIb8-9KwkKFLJxLnfDy4lweAe4xajKj8Ug5tirbFBGHK-AVYYSZ403WKXYIVUh1qFKHkI7gu5YAQEgqRK3ClKKuWWgG97V2cgg_WTCFFmDw0EW58gaEkn_IIp72Z4GDsa6lXB38-Udin0YRYwR7afU5jKlUY4GiOxxBfThn7eTzH3IIP3gzF3S3nDfj9bfNr_dQ8__i-XX99bizDcmq44FLw3lIujJPYCVWntt530ilnceerRaUjO69Y76Q5qUow3jFJkaGE3oDHc-4xpz-zK5MeQ7FuGEx0aS5aKMop69B_Qcxx_UlBK_jwD3hIc451CU04w4QiiU9Ue6ZsTqVk5_Uxh9HkN42RPhWky0HXgvRSUH3waYmdd6Pr3_Glkep_XnxTrBl8NtGG8o4RyRgniv4FSMyX8A</recordid><startdate>19971002</startdate><enddate>19971002</enddate><creator>ISHINO, M</creator><creator>OHBA, T</creator><creator>INAZAWA, J</creator><creator>SASAKI, H</creator><creator>ARIYAMA, Y</creator><creator>SASAKI, T</creator><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19971002</creationdate><title>Identification of an Efs isoform that lacks the SH3 domain and chromosomal mapping of human Efs</title><author>ISHINO, M ; OHBA, T ; INAZAWA, J ; SASAKI, H ; ARIYAMA, Y ; SASAKI, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-676876dc367ae81e79923cff58e9ec15fdc338e2bf94de8ae9ec974654830a323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adaptor Proteins, Signal Transducing</topic><topic>Alternative Splicing</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Chromosome 14</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 14</topic><topic>Cloning</topic><topic>Complementary DNA</topic><topic>Fluorescence in situ hybridization</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fyn protein</topic><topic>Gene mapping</topic><topic>Hippocampus</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Immunoprecipitation</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Phosphoproteins - genetics</topic><topic>Polymerase chain reaction</topic><topic>Proline</topic><topic>Protein Binding</topic><topic>Reverse transcription</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Alignment</topic><topic>Sequence Deletion</topic><topic>Signal transduction</topic><topic>src Homology Domains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ISHINO, M</creatorcontrib><creatorcontrib>OHBA, T</creatorcontrib><creatorcontrib>INAZAWA, J</creatorcontrib><creatorcontrib>SASAKI, H</creatorcontrib><creatorcontrib>ARIYAMA, Y</creatorcontrib><creatorcontrib>SASAKI, T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Oncogene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ISHINO, M</au><au>OHBA, T</au><au>INAZAWA, J</au><au>SASAKI, H</au><au>ARIYAMA, Y</au><au>SASAKI, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of an Efs isoform that lacks the SH3 domain and chromosomal mapping of human Efs</atitle><jtitle>Oncogene</jtitle><addtitle>Oncogene</addtitle><date>1997-10-02</date><risdate>1997</risdate><volume>15</volume><issue>14</issue><spage>1741</spage><epage>1745</epage><pages>1741-1745</pages><issn>0950-9232</issn><eissn>1476-5594</eissn><abstract>Efs was originally found by expression cloning of a mouse embryo cDNA library through its Fyn-SH3 binding capacity (Ishino et al., Oncogene 11, 2331-2338, 1995). Efs has characteristic regions important in intracellular signal transduction; these are an SH3 domain, a cluster of putative ligands for SH2 domains and proline-rich sequences with SH3-binding consensus. In this paper, we report cDNA cloning of human Efs and a variant of it from a hippocampal cDNA library. The human Efs gene was mapped to chromosome 14q11.2-q12 by fluorescence in situ hybridization. We identified two forms of human Efs, designated hEfs1 and hEfs2. hEfs1 represents the human counterpart of original mouse embryo Efs (mEfs1). hEfs2, the newly identified form, is identical to hEfs1, except for its lack of the SH3 domain. hEfs1 and mEfs1 are 80% identical in their amino acid sequences and 100% identical within the SH3 domain. Reverse transcription polymerase chain reaction analysis of adult mouse tissue RNA indicated expression of Efs2 and of Efs1 in various tissues. 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| source | MEDLINE; Nature; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature - Complete Springer Journals |
| subjects | Adaptor Proteins, Signal Transducing Alternative Splicing Amino Acid Sequence Animals Base Sequence Biological and medical sciences Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Chromosome 14 Chromosome Mapping Chromosomes, Human, Pair 14 Cloning Complementary DNA Fluorescence in situ hybridization Fundamental and applied biological sciences. Psychology Fyn protein Gene mapping Hippocampus Humans Immunoblotting Immunoprecipitation Mice Molecular and cellular biology Molecular Sequence Data Phosphoproteins - genetics Polymerase chain reaction Proline Protein Binding Reverse transcription RNA, Messenger - genetics Sequence Alignment Sequence Deletion Signal transduction src Homology Domains |
| title | Identification of an Efs isoform that lacks the SH3 domain and chromosomal mapping of human Efs |
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