Encapsidated Conformation of Bacteriophage T7 DNA
The structural organization of encapsidated T7 DNA was investigated by cryo-electron microscopy and image processing. A tail-deletion mutant was found to present two preferred views of phage heads: views along the axis through the capsid vertex where the connector protein resides and via which DNA i...
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| Veröffentlicht in: | Cell 1997-10, Vol.91 (2), p.271-280 |
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| container_title | Cell |
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| creator | Cerritelli, Mario E Cheng, Naiqian Rosenberg, Alan H McPherson, Catherine E Booy, Frank P Steven, Alasdair C |
| description | The structural organization of encapsidated T7 DNA was investigated by cryo-electron microscopy and image processing. A tail-deletion mutant was found to present two preferred views of phage heads: views along the axis through the capsid vertex where the connector protein resides and via which DNA is packaged; and side views perpendicular to this axis. The resulting images reveal striking patterns of concentric rings in axial views, and punctate arrays in side views. As corroborated by computer modeling, these data establish that the T7 chromosome is spooled around this axis in approximately six coaxial shells in a quasi-crystalline packing, possibly guided by the core complex on the inner surface of the connector. |
| doi_str_mv | 10.1016/S0092-8674(00)80409-2 |
| format | Article |
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A tail-deletion mutant was found to present two preferred views of phage heads: views along the axis through the capsid vertex where the connector protein resides and via which DNA is packaged; and side views perpendicular to this axis. The resulting images reveal striking patterns of concentric rings in axial views, and punctate arrays in side views. 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A tail-deletion mutant was found to present two preferred views of phage heads: views along the axis through the capsid vertex where the connector protein resides and via which DNA is packaged; and side views perpendicular to this axis. The resulting images reveal striking patterns of concentric rings in axial views, and punctate arrays in side views. As corroborated by computer modeling, these data establish that the T7 chromosome is spooled around this axis in approximately six coaxial shells in a quasi-crystalline packing, possibly guided by the core complex on the inner surface of the connector.</description><subject>Bacteriophage T7 - genetics</subject><subject>Bacteriophage T7 - ultrastructure</subject><subject>Capsid - chemistry</subject><subject>Capsid - ultrastructure</subject><subject>DNA, Viral - chemistry</subject><subject>DNA, Viral - ultrastructure</subject><subject>Escherichia coli</subject><subject>Genome, Viral</subject><subject>Image Processing, Computer-Assisted</subject><subject>Microscopy, Electron</subject><subject>Nucleic Acid Conformation</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EgvL4BKSsECwC40eceIWglIeEYAGsLXc8AaM2LnaKxN8TaMWW1SzuuXOlw9ghh1MOXJ89ARhRNrpWxwAnDSgwpdhgIw6mLhWvxSYb_SE7bDfndwBoqqraZttGKi2UGjE-6dAtcvCuJ1-MY9fGNHd9iF0R2-LSYU8pxMWbe6XiuS6uHi722VbrZpkO1nePvVxPnse35f3jzd344r7EqtJ9yZ0300pPazAapapJ8RZRti2hMIhc8sah0h58Ix33aETjvNOyUlNCbLTcY0erv4sUP5aUezsPGWk2cx3FZba1kVo0Qv4Lcj1AnMMAVisQU8w5UWsXKcxd-rIc7I9T--vU_gizAPbXqRVD73A9sJzOyf-11hKH_HyV06DjM1CyGQN1SD4kwt76GP5Z-AZZdITX</recordid><startdate>19971017</startdate><enddate>19971017</enddate><creator>Cerritelli, Mario E</creator><creator>Cheng, Naiqian</creator><creator>Rosenberg, Alan H</creator><creator>McPherson, Catherine E</creator><creator>Booy, Frank P</creator><creator>Steven, Alasdair C</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19971017</creationdate><title>Encapsidated Conformation of Bacteriophage T7 DNA</title><author>Cerritelli, Mario E ; Cheng, Naiqian ; Rosenberg, Alan H ; McPherson, Catherine E ; Booy, Frank P ; Steven, Alasdair C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-1ad9b56b7096c347e41fcc3ffec29cc1318ac46d0d83a1dc928ada6354becc863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Bacteriophage T7 - genetics</topic><topic>Bacteriophage T7 - ultrastructure</topic><topic>Capsid - chemistry</topic><topic>Capsid - ultrastructure</topic><topic>DNA, Viral - chemistry</topic><topic>DNA, Viral - ultrastructure</topic><topic>Escherichia coli</topic><topic>Genome, Viral</topic><topic>Image Processing, Computer-Assisted</topic><topic>Microscopy, Electron</topic><topic>Nucleic Acid Conformation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cerritelli, Mario E</creatorcontrib><creatorcontrib>Cheng, Naiqian</creatorcontrib><creatorcontrib>Rosenberg, Alan H</creatorcontrib><creatorcontrib>McPherson, Catherine E</creatorcontrib><creatorcontrib>Booy, Frank P</creatorcontrib><creatorcontrib>Steven, Alasdair C</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cerritelli, Mario E</au><au>Cheng, Naiqian</au><au>Rosenberg, Alan H</au><au>McPherson, Catherine E</au><au>Booy, Frank P</au><au>Steven, Alasdair C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Encapsidated Conformation of Bacteriophage T7 DNA</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1997-10-17</date><risdate>1997</risdate><volume>91</volume><issue>2</issue><spage>271</spage><epage>280</epage><pages>271-280</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><abstract>The structural organization of encapsidated T7 DNA was investigated by cryo-electron microscopy and image processing. A tail-deletion mutant was found to present two preferred views of phage heads: views along the axis through the capsid vertex where the connector protein resides and via which DNA is packaged; and side views perpendicular to this axis. The resulting images reveal striking patterns of concentric rings in axial views, and punctate arrays in side views. As corroborated by computer modeling, these data establish that the T7 chromosome is spooled around this axis in approximately six coaxial shells in a quasi-crystalline packing, possibly guided by the core complex on the inner surface of the connector.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9346244</pmid><doi>10.1016/S0092-8674(00)80409-2</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cell, 1997-10, Vol.91 (2), p.271-280 |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Bacteriophage T7 - genetics Bacteriophage T7 - ultrastructure Capsid - chemistry Capsid - ultrastructure DNA, Viral - chemistry DNA, Viral - ultrastructure Escherichia coli Genome, Viral Image Processing, Computer-Assisted Microscopy, Electron Nucleic Acid Conformation |
| title | Encapsidated Conformation of Bacteriophage T7 DNA |
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