Detection of acidic fibroblast growth factor mRNA in the rat ovary using reverse transcription-polymerase chain reaction amplification

We have examined the expression of acidic fibroblast growth factor (aFGF) in the rat ovary using reverse transcription-polymerase chain reaction (RT-PCR). RNA was extracted from hypothalami of adult rats and whole ovaries or isolated granulosa cells of gonadotropin-primed immature rats. The RNA was...

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Veröffentlicht in:	Biochemical and biophysical research communications 1989-11, Vol.165 (1), p.82-88
Hauptverfasser:	Koos, Robert D., Seidel, Richard H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins - genetics Animals Base Sequence Biological and medical sciences Brain - metabolism Female Fibroblast Growth Factors - genetics Fundamental and applied biological sciences. Psychology Granulosa Cells - metabolism Molecular and cellular biology Molecular genetics Molecular Sequence Data Moloney murine leukemia virus - enzymology Nucleic Acid Amplification Techniques Oligonucleotide Probes - chemical synthesis Ovary - metabolism Polymerase Chain Reaction - methods Rats Rats, Inbred Strains RNA, Messenger - analysis RNA, Messenger - genetics RNA-Directed DNA Polymerase Transcription. Transcription factor. Splicing. Rna processing
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