Detection of total DNA with single-stranded DNA binding protein conjugates

We have developed a rapid and sensitive method for total DNA measurement using single-stranded DNA binding protein from E. coli conjugated with horseradish peroxidase or urease. To detect DNA, the sample is heated or alkali treated to denature the DNA and then filtered through nylon or nitrocellulos...

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Veröffentlicht in:	Biochemical and biophysical research communications 1989-11, Vol.165 (1), p.474-480
Hauptverfasser:	Sheldon, Edward L., Nagainis, Peter A., Kung, Viola T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Colorimetry - methods DNA DNA - analysis DNA - metabolism DNA, Bacterial - analysis Dna, deoxyribonucleoproteins DNA-Binding Proteins - metabolism Escherichia coli - isolation & purification Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Nucleic acids Urease - metabolism Water Microbiology
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