Modulation of Protein Kinase C Activity in NIH 3T3 Cells by Plant Glycosides from Panax ginseng
Abstract The involvement of ginsenosides in the signal cascade that stimulates cellular growth was investigated. It was found that ginsenosides Rh 1 and Rh 2 extracted from the root of PANAX GINSENG inhibited cellular proliferation in NIH 3T3 fibroblasts. Both ginsenosides Rh 1 and Rh 2 effectively...
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| Veröffentlicht in: | Planta medica 1997-10, Vol.63 (5), p.389-392 |
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| container_title | Planta medica |
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| creator | Byun, Boo Hyeong Shin, Incheol Yoon, Yoo-Sik Kim, Shin IL Joe, Cheol O |
| description | Abstract
The involvement of ginsenosides in the signal cascade that stimulates cellular growth was investigated. It was found that ginsenosides Rh
1
and Rh
2
extracted from the root of PANAX GINSENG inhibited cellular proliferation in NIH 3T3 fibroblasts. Both ginsenosides Rh
1
and Rh
2
effectively reduced phospholipase C activity resulting in a decrease in the intracellular level of diacylglycerol, an endogenous activator of protein kinase C. The treatment of cells with Rh
1
or Rh
2
was thus found to reduce intracellular protein kinase C activity. We also observed that the phosphorylation of myristoylated alanine-rich C kinase substrate, one of the major substrates of protein kinase C in cells, was inhibited by the ginsenosides. Data suggest that the ginsenoside Rh
1
or Rh
2
exerts antiproliferative effects by inhibiting phospholipase C, which produces second messengers necessary for the activation of protein kinase C. |
| doi_str_mv | 10.1055/s-2006-957719 |
| format | Article |
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The involvement of ginsenosides in the signal cascade that stimulates cellular growth was investigated. It was found that ginsenosides Rh
1
and Rh
2
extracted from the root of PANAX GINSENG inhibited cellular proliferation in NIH 3T3 fibroblasts. Both ginsenosides Rh
1
and Rh
2
effectively reduced phospholipase C activity resulting in a decrease in the intracellular level of diacylglycerol, an endogenous activator of protein kinase C. The treatment of cells with Rh
1
or Rh
2
was thus found to reduce intracellular protein kinase C activity. We also observed that the phosphorylation of myristoylated alanine-rich C kinase substrate, one of the major substrates of protein kinase C in cells, was inhibited by the ginsenosides. Data suggest that the ginsenoside Rh
1
or Rh
2
exerts antiproliferative effects by inhibiting phospholipase C, which produces second messengers necessary for the activation of protein kinase C.</description><identifier>ISSN: 0032-0943</identifier><identifier>EISSN: 1439-0221</identifier><identifier>DOI: 10.1055/s-2006-957719</identifier><identifier>PMID: 9342939</identifier><identifier>CODEN: PLMEAA</identifier><language>eng</language><publisher>Stuttgart: Thieme</publisher><subject>3T3 Cells ; Animals ; Biological and medical sciences ; Cell Division - drug effects ; Diglycerides - metabolism ; General pharmacology ; Ginsenosides ; Glycosides - pharmacology ; Medical sciences ; Mice ; Panax - chemistry ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Plants, Medicinal ; Protein Kinase C - metabolism ; Saponins - pharmacology ; Type C Phospholipases - antagonists & inhibitors</subject><ispartof>Planta medica, 1997-10, Vol.63 (5), p.389-392</ispartof><rights>Georg Thieme Verlag Stuttgart · New York</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2699-17645d8656000136e9b0ac9a262eb0b17b715b3b2a9604bfa05fd71828262a853</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2006-957719.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><link.rule.ids>314,776,780,3004,3005,27901,27902,54534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2810748$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9342939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Byun, Boo Hyeong</creatorcontrib><creatorcontrib>Shin, Incheol</creatorcontrib><creatorcontrib>Yoon, Yoo-Sik</creatorcontrib><creatorcontrib>Kim, Shin IL</creatorcontrib><creatorcontrib>Joe, Cheol O</creatorcontrib><title>Modulation of Protein Kinase C Activity in NIH 3T3 Cells by Plant Glycosides from Panax ginseng</title><title>Planta medica</title><addtitle>Planta Med</addtitle><description>Abstract
The involvement of ginsenosides in the signal cascade that stimulates cellular growth was investigated. It was found that ginsenosides Rh
1
and Rh
2
extracted from the root of PANAX GINSENG inhibited cellular proliferation in NIH 3T3 fibroblasts. Both ginsenosides Rh
1
and Rh
2
effectively reduced phospholipase C activity resulting in a decrease in the intracellular level of diacylglycerol, an endogenous activator of protein kinase C. The treatment of cells with Rh
1
or Rh
2
was thus found to reduce intracellular protein kinase C activity. We also observed that the phosphorylation of myristoylated alanine-rich C kinase substrate, one of the major substrates of protein kinase C in cells, was inhibited by the ginsenosides. Data suggest that the ginsenoside Rh
1
or Rh
2
exerts antiproliferative effects by inhibiting phospholipase C, which produces second messengers necessary for the activation of protein kinase C.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Division - drug effects</subject><subject>Diglycerides - metabolism</subject><subject>General pharmacology</subject><subject>Ginsenosides</subject><subject>Glycosides - pharmacology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Panax - chemistry</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Plants, Medicinal</subject><subject>Protein Kinase C - metabolism</subject><subject>Saponins - pharmacology</subject><subject>Type C Phospholipases - antagonists & inhibitors</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEFv1DAQRi0EKtvCkSOSD4gTgbEd2_GxWpW2osAeytmyE6e4SuziSRD773G1q956Gmm-p29Gj5B3DD4zkPILNhxANUZqzcwLsmGtMA1wzl6SDYDgDZhWvCaniPcArDUAJ-TEiJYbYTbEfs_DOrkl5kTzSHclLyEm-i0mh4Fu6Xm_xL9x2dO6_HF9RcWtoNswTUj9nu4mlxZ6Oe37jHEISMeSZ7pzyf2jdzFhSHdvyKvRTRjeHucZ-fX14nZ71dz8vLzent80PVfGNEyrVg6dkgrqk0IF48H1xnHFgwfPtNdMeuG5MwpaPzqQ46BZx7tKuE6KM_Lx0PtQ8p814GLniH191KWQV7TaCKmE1hVsDmBfMmIJo30ocXZlbxnYR6EW7aNQexBa-ffH4tXPYXiijwZr_uGYO-zdNBaX-ohPGO8Y6Lar2KcDtvyOYQ72Pq8lVSHPXP0PEA6IbQ</recordid><startdate>199710</startdate><enddate>199710</enddate><creator>Byun, Boo Hyeong</creator><creator>Shin, Incheol</creator><creator>Yoon, Yoo-Sik</creator><creator>Kim, Shin IL</creator><creator>Joe, Cheol O</creator><general>Thieme</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199710</creationdate><title>Modulation of Protein Kinase C Activity in NIH 3T3 Cells by Plant Glycosides from Panax ginseng</title><author>Byun, Boo Hyeong ; Shin, Incheol ; Yoon, Yoo-Sik ; Kim, Shin IL ; Joe, Cheol O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2699-17645d8656000136e9b0ac9a262eb0b17b715b3b2a9604bfa05fd71828262a853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>3T3 Cells</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Division - drug effects</topic><topic>Diglycerides - metabolism</topic><topic>General pharmacology</topic><topic>Ginsenosides</topic><topic>Glycosides - pharmacology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Panax - chemistry</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Plants, Medicinal</topic><topic>Protein Kinase C - metabolism</topic><topic>Saponins - pharmacology</topic><topic>Type C Phospholipases - antagonists & inhibitors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Byun, Boo Hyeong</creatorcontrib><creatorcontrib>Shin, Incheol</creatorcontrib><creatorcontrib>Yoon, Yoo-Sik</creatorcontrib><creatorcontrib>Kim, Shin IL</creatorcontrib><creatorcontrib>Joe, Cheol O</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Byun, Boo Hyeong</au><au>Shin, Incheol</au><au>Yoon, Yoo-Sik</au><au>Kim, Shin IL</au><au>Joe, Cheol O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of Protein Kinase C Activity in NIH 3T3 Cells by Plant Glycosides from Panax ginseng</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>1997-10</date><risdate>1997</risdate><volume>63</volume><issue>5</issue><spage>389</spage><epage>392</epage><pages>389-392</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><coden>PLMEAA</coden><abstract>Abstract
The involvement of ginsenosides in the signal cascade that stimulates cellular growth was investigated. It was found that ginsenosides Rh
1
and Rh
2
extracted from the root of PANAX GINSENG inhibited cellular proliferation in NIH 3T3 fibroblasts. Both ginsenosides Rh
1
and Rh
2
effectively reduced phospholipase C activity resulting in a decrease in the intracellular level of diacylglycerol, an endogenous activator of protein kinase C. The treatment of cells with Rh
1
or Rh
2
was thus found to reduce intracellular protein kinase C activity. We also observed that the phosphorylation of myristoylated alanine-rich C kinase substrate, one of the major substrates of protein kinase C in cells, was inhibited by the ginsenosides. Data suggest that the ginsenoside Rh
1
or Rh
2
exerts antiproliferative effects by inhibiting phospholipase C, which produces second messengers necessary for the activation of protein kinase C.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>9342939</pmid><doi>10.1055/s-2006-957719</doi><tpages>4</tpages></addata></record> |
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| ispartof | Planta medica, 1997-10, Vol.63 (5), p.389-392 |
| issn | 0032-0943 1439-0221 |
| language | eng |
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| source | MEDLINE; Thieme Connect Journals |
| subjects | 3T3 Cells Animals Biological and medical sciences Cell Division - drug effects Diglycerides - metabolism General pharmacology Ginsenosides Glycosides - pharmacology Medical sciences Mice Panax - chemistry Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plants, Medicinal Protein Kinase C - metabolism Saponins - pharmacology Type C Phospholipases - antagonists & inhibitors |
| title | Modulation of Protein Kinase C Activity in NIH 3T3 Cells by Plant Glycosides from Panax ginseng |
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