Red cell distortion and conceptual basis of diffusing capacity estimates: finite element analysis
C. C. W. Hsia 1 , C. J. C. Chuong 2 , and R. L. Johnson Jr. 1 1 Department of Medicine, University of Texas Southwestern Medical Center, Dallas 75235; and 2 Biomedical Engineering Program, University of Texas at Arlington, Arlington, Texas 76019 Received 31 January 1997; accepted in final form 2 J...
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| creator | Hsia, C. C. W Chuong, C. J. C Johnson, R. L., Jr |
| description | C. C. W.
Hsia 1 ,
C. J. C.
Chuong 2 , and
R. L.
Johnson Jr. 1
1 Department of Medicine,
University of Texas Southwestern Medical Center, Dallas 75235; and
2 Biomedical Engineering Program,
University of Texas at Arlington, Arlington, Texas 76019
Received 31 January 1997; accepted in final form 2 June 1997.
Hsia, C. C. W., C. J. C. Chuong, and R. L. Johnson, Jr.
Red cell distortion and conceptual basis of diffusing capacity estimates: finite element analysis. J. Appl.
Physiol. 83(4): 1397-1404, 1997. To understand
the effects of dynamic shape distortion of red blood cells (RBCs) as it
develops under high-flow conditions on the standard physiological and
morphometric methods of estimating pulmonary diffusing capacity, we
computed the uptake of CO across a two-dimensional geometric capillary
model containing a variable number of equally spaced RBCs. RBCs are
circular or parachute shaped, with the same perimeter length. Total CO
diffusing capacity (D L CO )
and membrane diffusing capacity
(D M CO )
were calculated by a finite element method.
D L CO
calculated at two levels of alveolar P O 2 were used to estimate
D M CO by the
Roughton-Forster (RF) technique. The same capillary model was subjected
to morphometric analysis by the random linear intercept method to
obtain morphometric estimates of
D M CO . Results show that
shape distortion of RBCs significantly reduces capillary diffusive gas
uptake. Shape distortion exaggerates the conceptual errors inherent in
the RF technique ( J. Appl. Physiol.
79: 1039-1047, 1995); errors are exaggerated at a high capillary
hematocrit. Shape distortion also introduces additional error in
morphometric estimates of
D M CO caused
by a biased sampling distribution of random linear intercepts; errors are exaggerated at a low capillary hematocrit.
Roughton-Forster technique; morphometry; pulmonary diffusing
capacity; membrane diffusing capacity; random linear intercept; capillary model
0161-7567/97 $5.00
Copyright © 1997 the American Physiological Society |
| doi_str_mv | 10.1152/jappl.1997.83.4.1397 |
| format | Article |
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Hsia 1 ,
C. J. C.
Chuong 2 , and
R. L.
Johnson Jr. 1
1 Department of Medicine,
University of Texas Southwestern Medical Center, Dallas 75235; and
2 Biomedical Engineering Program,
University of Texas at Arlington, Arlington, Texas 76019
Received 31 January 1997; accepted in final form 2 June 1997.
Hsia, C. C. W., C. J. C. Chuong, and R. L. Johnson, Jr.
Red cell distortion and conceptual basis of diffusing capacity estimates: finite element analysis. J. Appl.
Physiol. 83(4): 1397-1404, 1997. To understand
the effects of dynamic shape distortion of red blood cells (RBCs) as it
develops under high-flow conditions on the standard physiological and
morphometric methods of estimating pulmonary diffusing capacity, we
computed the uptake of CO across a two-dimensional geometric capillary
model containing a variable number of equally spaced RBCs. RBCs are
circular or parachute shaped, with the same perimeter length. Total CO
diffusing capacity (D L CO )
and membrane diffusing capacity
(D M CO )
were calculated by a finite element method.
D L CO
calculated at two levels of alveolar P O 2 were used to estimate
D M CO by the
Roughton-Forster (RF) technique. The same capillary model was subjected
to morphometric analysis by the random linear intercept method to
obtain morphometric estimates of
D M CO . Results show that
shape distortion of RBCs significantly reduces capillary diffusive gas
uptake. Shape distortion exaggerates the conceptual errors inherent in
the RF technique ( J. Appl. Physiol.
79: 1039-1047, 1995); errors are exaggerated at a high capillary
hematocrit. Shape distortion also introduces additional error in
morphometric estimates of
D M CO caused
by a biased sampling distribution of random linear intercepts; errors are exaggerated at a low capillary hematocrit.
Roughton-Forster technique; morphometry; pulmonary diffusing
capacity; membrane diffusing capacity; random linear intercept; capillary model
0161-7567/97 $5.00
Copyright © 1997 the American Physiological Society</description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/jappl.1997.83.4.1397</identifier><identifier>PMID: 9338451</identifier><identifier>CODEN: JAPHEV</identifier><language>eng</language><publisher>Bethesda, MD: Am Physiological Soc</publisher><subject>Algorithms ; Biological and medical sciences ; Capillaries - anatomy & histology ; Capillaries - physiology ; Carbon Monoxide - blood ; Erythrocyte Count ; Erythrocyte Deformability - physiology ; Erythrocyte Membrane - metabolism ; Erythrocytes - physiology ; Erythrocytes - ultrastructure ; Fundamental and applied biological sciences. Psychology ; Humans ; In Vitro Techniques ; Models, Biological ; Pulmonary Diffusing Capacity - physiology ; Vertebrates: blood, hematopoietic organs, reticuloendothelial system</subject><ispartof>Journal of applied physiology (1985), 1997-10, Vol.83 (4), p.1397-1404</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-63a215fe6d35ac6fcb9ec416047ddcfa9cedb7356b80cb75dc5f8aa4772890723</citedby><cites>FETCH-LOGICAL-c409t-63a215fe6d35ac6fcb9ec416047ddcfa9cedb7356b80cb75dc5f8aa4772890723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2842628$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9338451$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hsia, C. C. W</creatorcontrib><creatorcontrib>Chuong, C. J. C</creatorcontrib><creatorcontrib>Johnson, R. L., Jr</creatorcontrib><title>Red cell distortion and conceptual basis of diffusing capacity estimates: finite element analysis</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description>C. C. W.
Hsia 1 ,
C. J. C.
Chuong 2 , and
R. L.
Johnson Jr. 1
1 Department of Medicine,
University of Texas Southwestern Medical Center, Dallas 75235; and
2 Biomedical Engineering Program,
University of Texas at Arlington, Arlington, Texas 76019
Received 31 January 1997; accepted in final form 2 June 1997.
Hsia, C. C. W., C. J. C. Chuong, and R. L. Johnson, Jr.
Red cell distortion and conceptual basis of diffusing capacity estimates: finite element analysis. J. Appl.
Physiol. 83(4): 1397-1404, 1997. To understand
the effects of dynamic shape distortion of red blood cells (RBCs) as it
develops under high-flow conditions on the standard physiological and
morphometric methods of estimating pulmonary diffusing capacity, we
computed the uptake of CO across a two-dimensional geometric capillary
model containing a variable number of equally spaced RBCs. RBCs are
circular or parachute shaped, with the same perimeter length. Total CO
diffusing capacity (D L CO )
and membrane diffusing capacity
(D M CO )
were calculated by a finite element method.
D L CO
calculated at two levels of alveolar P O 2 were used to estimate
D M CO by the
Roughton-Forster (RF) technique. The same capillary model was subjected
to morphometric analysis by the random linear intercept method to
obtain morphometric estimates of
D M CO . Results show that
shape distortion of RBCs significantly reduces capillary diffusive gas
uptake. Shape distortion exaggerates the conceptual errors inherent in
the RF technique ( J. Appl. Physiol.
79: 1039-1047, 1995); errors are exaggerated at a high capillary
hematocrit. Shape distortion also introduces additional error in
morphometric estimates of
D M CO caused
by a biased sampling distribution of random linear intercepts; errors are exaggerated at a low capillary hematocrit.
Roughton-Forster technique; morphometry; pulmonary diffusing
capacity; membrane diffusing capacity; random linear intercept; capillary model
0161-7567/97 $5.00
Copyright © 1997 the American Physiological Society</description><subject>Algorithms</subject><subject>Biological and medical sciences</subject><subject>Capillaries - anatomy & histology</subject><subject>Capillaries - physiology</subject><subject>Carbon Monoxide - blood</subject><subject>Erythrocyte Count</subject><subject>Erythrocyte Deformability - physiology</subject><subject>Erythrocyte Membrane - metabolism</subject><subject>Erythrocytes - physiology</subject><subject>Erythrocytes - ultrastructure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Models, Biological</subject><subject>Pulmonary Diffusing Capacity - physiology</subject><subject>Vertebrates: blood, hematopoietic organs, reticuloendothelial system</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UE1v1DAUtBCobAv_AKQcEOolwY7t2OaGKgpIlSpV5Wy9-GPXlTcJsSPYf49DV0svPfnJM_PezCD0juCGEN5-eoBpig1RSjSSNqwhVIkXaFOgtiYdJi_RRgqOa8GleI3OU3rAmDDGyRk6U5TKMm0Q3DlbGRdjZUPK45zDOFQwlL9xMG7KC8SqhxRSNfpC8X5JYdhWBiYwIR8ql3LYQ3bpc-XDELKrXHR7N-SyBOKhCN-gVx5icm-P7wX6ef31_up7fXP77cfVl5vaMKxy3VFoCfeus5SD6bzplTOs5GDCWuNBGWd7QXnXS2x6wa3hXgIwIVqpsGjpBfr4uHeax19L8aX3Ia3JYHDjkrRQlHW0w4XIHolmHlOandfTXDLMB02wXpvV_5rVa7NaUs302myRvT_uX_q9syfRscqCfzjikAxEP8NgQjrRWsnarpX_r-_Cdvc7zE5Pu1LTGMftQV8vMd67P3l1cLqsJ-uL7PJ5WWE_MfoX9JumCA</recordid><startdate>19971001</startdate><enddate>19971001</enddate><creator>Hsia, C. C. W</creator><creator>Chuong, C. J. C</creator><creator>Johnson, R. L., Jr</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19971001</creationdate><title>Red cell distortion and conceptual basis of diffusing capacity estimates: finite element analysis</title><author>Hsia, C. C. W ; Chuong, C. J. C ; Johnson, R. L., Jr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-63a215fe6d35ac6fcb9ec416047ddcfa9cedb7356b80cb75dc5f8aa4772890723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Algorithms</topic><topic>Biological and medical sciences</topic><topic>Capillaries - anatomy & histology</topic><topic>Capillaries - physiology</topic><topic>Carbon Monoxide - blood</topic><topic>Erythrocyte Count</topic><topic>Erythrocyte Deformability - physiology</topic><topic>Erythrocyte Membrane - metabolism</topic><topic>Erythrocytes - physiology</topic><topic>Erythrocytes - ultrastructure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Models, Biological</topic><topic>Pulmonary Diffusing Capacity - physiology</topic><topic>Vertebrates: blood, hematopoietic organs, reticuloendothelial system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hsia, C. C. W</creatorcontrib><creatorcontrib>Chuong, C. J. C</creatorcontrib><creatorcontrib>Johnson, R. L., Jr</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hsia, C. C. W</au><au>Chuong, C. J. C</au><au>Johnson, R. L., Jr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Red cell distortion and conceptual basis of diffusing capacity estimates: finite element analysis</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>1997-10-01</date><risdate>1997</risdate><volume>83</volume><issue>4</issue><spage>1397</spage><epage>1404</epage><pages>1397-1404</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><coden>JAPHEV</coden><abstract>C. C. W.
Hsia 1 ,
C. J. C.
Chuong 2 , and
R. L.
Johnson Jr. 1
1 Department of Medicine,
University of Texas Southwestern Medical Center, Dallas 75235; and
2 Biomedical Engineering Program,
University of Texas at Arlington, Arlington, Texas 76019
Received 31 January 1997; accepted in final form 2 June 1997.
Hsia, C. C. W., C. J. C. Chuong, and R. L. Johnson, Jr.
Red cell distortion and conceptual basis of diffusing capacity estimates: finite element analysis. J. Appl.
Physiol. 83(4): 1397-1404, 1997. To understand
the effects of dynamic shape distortion of red blood cells (RBCs) as it
develops under high-flow conditions on the standard physiological and
morphometric methods of estimating pulmonary diffusing capacity, we
computed the uptake of CO across a two-dimensional geometric capillary
model containing a variable number of equally spaced RBCs. RBCs are
circular or parachute shaped, with the same perimeter length. Total CO
diffusing capacity (D L CO )
and membrane diffusing capacity
(D M CO )
were calculated by a finite element method.
D L CO
calculated at two levels of alveolar P O 2 were used to estimate
D M CO by the
Roughton-Forster (RF) technique. The same capillary model was subjected
to morphometric analysis by the random linear intercept method to
obtain morphometric estimates of
D M CO . Results show that
shape distortion of RBCs significantly reduces capillary diffusive gas
uptake. Shape distortion exaggerates the conceptual errors inherent in
the RF technique ( J. Appl. Physiol.
79: 1039-1047, 1995); errors are exaggerated at a high capillary
hematocrit. Shape distortion also introduces additional error in
morphometric estimates of
D M CO caused
by a biased sampling distribution of random linear intercepts; errors are exaggerated at a low capillary hematocrit.
Roughton-Forster technique; morphometry; pulmonary diffusing
capacity; membrane diffusing capacity; random linear intercept; capillary model
0161-7567/97 $5.00
Copyright © 1997 the American Physiological Society</abstract><cop>Bethesda, MD</cop><pub>Am Physiological Soc</pub><pmid>9338451</pmid><doi>10.1152/jappl.1997.83.4.1397</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 8750-7587 |
| ispartof | Journal of applied physiology (1985), 1997-10, Vol.83 (4), p.1397-1404 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_79346360 |
| source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Algorithms Biological and medical sciences Capillaries - anatomy & histology Capillaries - physiology Carbon Monoxide - blood Erythrocyte Count Erythrocyte Deformability - physiology Erythrocyte Membrane - metabolism Erythrocytes - physiology Erythrocytes - ultrastructure Fundamental and applied biological sciences. Psychology Humans In Vitro Techniques Models, Biological Pulmonary Diffusing Capacity - physiology Vertebrates: blood, hematopoietic organs, reticuloendothelial system |
| title | Red cell distortion and conceptual basis of diffusing capacity estimates: finite element analysis |
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