Minimum length of sequence homology required for in vivo cloning by homologous recombination in yeast

With efficient homologous recombination in Saccharomyces cerevisiae, a rapid in vivo cloning technique has been available. Here we demonstrated that 30 bp of a homologous sequence at each end of a DNA fragment is sufficient to integrate the fragment into a linearized plasmid in yeast. To obtain a hi...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Plasmid 1997, Vol.38 (2), p.91-96
Hauptverfasser:	Hua, S B, Qiu, M, Chan, E, Zhu, L, Luo, Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Cloning, Molecular - methods DNA Primers Mice Molecular Sequence Data Polymerase Chain Reaction - methods Recombination, Genetic Saccharomyces cerevisiae - genetics Sequence Homology, Nucleic Acid Transformation, Genetic Tumor Suppressor Protein p53 - genetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!