Overproduction and purification of an agarase of bacterial origin

The agarase gene from Streptomyces coelicolor has been cloned in the non-producer bacterium Streptomyces lividans under the control of its own set of promoters and under the control of a heterologous promoter that is functional only during exponential growth. The best level of overproduction was obt...

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Veröffentlicht in:	Journal of biotechnology 1997-10, Vol.58 (1), p.59-66
Hauptverfasser:	Parro, Vı́ctor, Vives, Carme, Godia, Francesc, Mellado, Rafael P
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity purification Agarase Bacterial Proteins - biosynthesis Biological and medical sciences Biotechnology DNA - isolation & purification Enzyme engineering Exponential phase promoter Fed-batch culture Fundamental and applied biological sciences. Psychology Glycoside Hydrolases - biosynthesis Glycoside Hydrolases - genetics Glycoside Hydrolases - isolation & purification Improved methods for extraction and purification of enzymes Methods. Procedures. Technologies Overproduction Promoter Regions, Genetic Streptomyces - genetics Streptomyces coelicolor
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