Fast Ion-Exchange HPLC of Proteins Using Porous Poly(glycidyl methacrylate-co-ethylene dimethacrylate) Monoliths Grafted with Poly(2-acrylamido-2-methyl-1-propanesulfonic acid)
Porous poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) monoliths with different porous properties grafted with poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid) chains using cerium(IV) initiated free‐radical polymerization have been prepared and used for the separation of proteins in ion‐exc...
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| Veröffentlicht in: | Biotechnology progress 1997, Vol.13 (5), p.597-600 |
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| container_title | Biotechnology progress |
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| creator | Viklund, Camilla Svec, Frantisek Fréchet, Jean M. J. Irgum, Knut |
| description | Porous poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) monoliths with different porous properties grafted with poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid) chains using cerium(IV) initiated free‐radical polymerization have been prepared and used for the separation of proteins in ion‐exchange HPLC mode. Because of the presence of the large pores that are typical of monolithic separation media which allow easy flow of all of the mobile phase, the efficiency of the columns does not deteriorate even at high flow velocities as a result of the specific morphology of the monoliths. Optimization of the chromatographic conditions such as the shape of the mobile phase gradient and the flow rate allows for very fast separation of three proteins in less than 1.5 min. |
| doi_str_mv | 10.1021/bp9700667 |
| format | Article |
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J.</creatorcontrib><creatorcontrib>Irgum, Knut</creatorcontrib><title>Fast Ion-Exchange HPLC of Proteins Using Porous Poly(glycidyl methacrylate-co-ethylene dimethacrylate) Monoliths Grafted with Poly(2-acrylamido-2-methyl-1-propanesulfonic acid)</title><title>Biotechnology progress</title><addtitle>Biotechnol Progress</addtitle><description>Porous poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) monoliths with different porous properties grafted with poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid) chains using cerium(IV) initiated free‐radical polymerization have been prepared and used for the separation of proteins in ion‐exchange HPLC mode. Because of the presence of the large pores that are typical of monolithic separation media which allow easy flow of all of the mobile phase, the efficiency of the columns does not deteriorate even at high flow velocities as a result of the specific morphology of the monoliths. Optimization of the chromatographic conditions such as the shape of the mobile phase gradient and the flow rate allows for very fast separation of three proteins in less than 1.5 min.</description><subject>Adsorption</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chymotrypsinogen - isolation & purification</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Indicators and Reagents</subject><subject>Methods. Procedures. 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Technologies</topic><topic>Methylmethacrylates</topic><topic>Muramidase - isolation & purification</topic><topic>Myoglobin - isolation & purification</topic><topic>Organic polymers</topic><topic>Others</topic><topic>Permeability</topic><topic>Physicochemistry of polymers</topic><topic>Polymers</topic><topic>Polymers with particular properties</topic><topic>Preparation, kinetics, thermodynamics, mechanism and catalysts</topic><topic>Proteins - isolation & purification</topic><topic>Sulfonic Acids</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Viklund, Camilla</creatorcontrib><creatorcontrib>Svec, Frantisek</creatorcontrib><creatorcontrib>Fréchet, Jean M. 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J.</au><au>Irgum, Knut</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fast Ion-Exchange HPLC of Proteins Using Porous Poly(glycidyl methacrylate-co-ethylene dimethacrylate) Monoliths Grafted with Poly(2-acrylamido-2-methyl-1-propanesulfonic acid)</atitle><jtitle>Biotechnology progress</jtitle><addtitle>Biotechnol Progress</addtitle><date>1997</date><risdate>1997</risdate><volume>13</volume><issue>5</issue><spage>597</spage><epage>600</epage><pages>597-600</pages><issn>8756-7938</issn><eissn>1520-6033</eissn><coden>BIPRET</coden><abstract>Porous poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) monoliths with different porous properties grafted with poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid) chains using cerium(IV) initiated free‐radical polymerization have been prepared and used for the separation of proteins in ion‐exchange HPLC mode. Because of the presence of the large pores that are typical of monolithic separation media which allow easy flow of all of the mobile phase, the efficiency of the columns does not deteriorate even at high flow velocities as a result of the specific morphology of the monoliths. Optimization of the chromatographic conditions such as the shape of the mobile phase gradient and the flow rate allows for very fast separation of three proteins in less than 1.5 min.</abstract><cop>USA</cop><pub>American Chemical Society</pub><pmid>9336979</pmid><doi>10.1021/bp9700667</doi><tpages>4</tpages></addata></record> |
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| ispartof | Biotechnology progress, 1997, Vol.13 (5), p.597-600 |
| issn | 8756-7938 1520-6033 |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Adsorption Applied sciences Biological and medical sciences Biotechnology Chromatography, High Pressure Liquid - methods Chymotrypsinogen - isolation & purification Exact sciences and technology Fundamental and applied biological sciences. Psychology Indicators and Reagents Methods. Procedures. Technologies Methylmethacrylates Muramidase - isolation & purification Myoglobin - isolation & purification Organic polymers Others Permeability Physicochemistry of polymers Polymers Polymers with particular properties Preparation, kinetics, thermodynamics, mechanism and catalysts Proteins - isolation & purification Sulfonic Acids Various methods and equipments |
| title | Fast Ion-Exchange HPLC of Proteins Using Porous Poly(glycidyl methacrylate-co-ethylene dimethacrylate) Monoliths Grafted with Poly(2-acrylamido-2-methyl-1-propanesulfonic acid) |
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