Pentylenetetrazole-induced changes of the single potassium channel in primary cultured cerebral cortical neurons
To elucidate the behavior of the single ionic channels of cerebral cortical neurons during bursting activity, the effects of pentylenetetrazole (PTZ) on a single potassium channel of primary cultured cerebral cortical neurons from mice were examined. All of the examined 10-day-old primary cultured n...
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| Veröffentlicht in: | Brain research 1989-09, Vol.497 (2), p.239-244 |
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| container_title | Brain research |
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| creator | Sugaya, Eiichi Sugaya, Aiko Takagi, Tamaki Tsuda, Tadashi Kajiwara, Kagemasa Yasuda, Kazumi Komatsubara, Junichi |
| description | To elucidate the behavior of the single ionic channels of cerebral cortical neurons during bursting activity, the effects of pentylenetetrazole (PTZ) on a single potassium channel of primary cultured cerebral cortical neurons from mice were examined. All of the examined 10-day-old primary cultured neurons of the cerebral cortex showed clear bursting activity after extracellular application of PTZ using whole-cell patch-clamp recording. Less than half of the examined single potassium channels, both outward and inward, of the 2- and 3-day-old as well as 6–10-day-old primary cultured cerebral cortical neurons showed the bursting-type open-close state by application of PTZ. The PTZ-sensitive single potassium channels were found in the voltage-dependent as well as calcium-activated channels. |
| doi_str_mv | 10.1016/0006-8993(89)90268-0 |
| format | Article |
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All of the examined 10-day-old primary cultured neurons of the cerebral cortex showed clear bursting activity after extracellular application of PTZ using whole-cell patch-clamp recording. Less than half of the examined single potassium channels, both outward and inward, of the 2- and 3-day-old as well as 6–10-day-old primary cultured cerebral cortical neurons showed the bursting-type open-close state by application of PTZ. The PTZ-sensitive single potassium channels were found in the voltage-dependent as well as calcium-activated channels.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(89)90268-0</identifier><identifier>PMID: 2819423</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Cells, Cultured ; Central nervous system ; Cerebral Cortex - physiology ; Cultured neuron ; Electric Conductivity ; Electrophysiology ; Embryo, Mammalian ; Evoked Potentials - drug effects ; Fundamental and applied biological sciences. Psychology ; Mice ; Mice, Inbred Strains ; Neurons - drug effects ; Neurons - physiology ; Patch clamp ; Pentylenetetrazole ; Pentylenetetrazole - pharmacology ; Potassium Channels - drug effects ; Potassium Channels - physiology ; Seizure ; Single potassium channel ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 1989-09, Vol.497 (2), p.239-244</ispartof><rights>1989 Elsevier Science Publishers B.V. (Biomedical Division)</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-39a43ecba3c85918ccfde4c96403565102e8088ff06a6f862c8dfb7dccfa7fba3</citedby><cites>FETCH-LOGICAL-c483t-39a43ecba3c85918ccfde4c96403565102e8088ff06a6f862c8dfb7dccfa7fba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006899389902680$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6812302$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2819423$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugaya, Eiichi</creatorcontrib><creatorcontrib>Sugaya, Aiko</creatorcontrib><creatorcontrib>Takagi, Tamaki</creatorcontrib><creatorcontrib>Tsuda, Tadashi</creatorcontrib><creatorcontrib>Kajiwara, Kagemasa</creatorcontrib><creatorcontrib>Yasuda, Kazumi</creatorcontrib><creatorcontrib>Komatsubara, Junichi</creatorcontrib><title>Pentylenetetrazole-induced changes of the single potassium channel in primary cultured cerebral cortical neurons</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>To elucidate the behavior of the single ionic channels of cerebral cortical neurons during bursting activity, the effects of pentylenetetrazole (PTZ) on a single potassium channel of primary cultured cerebral cortical neurons from mice were examined. All of the examined 10-day-old primary cultured neurons of the cerebral cortex showed clear bursting activity after extracellular application of PTZ using whole-cell patch-clamp recording. Less than half of the examined single potassium channels, both outward and inward, of the 2- and 3-day-old as well as 6–10-day-old primary cultured cerebral cortical neurons showed the bursting-type open-close state by application of PTZ. The PTZ-sensitive single potassium channels were found in the voltage-dependent as well as calcium-activated channels.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Central nervous system</subject><subject>Cerebral Cortex - physiology</subject><subject>Cultured neuron</subject><subject>Electric Conductivity</subject><subject>Electrophysiology</subject><subject>Embryo, Mammalian</subject><subject>Evoked Potentials - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>Patch clamp</subject><subject>Pentylenetetrazole</subject><subject>Pentylenetetrazole - pharmacology</subject><subject>Potassium Channels - drug effects</subject><subject>Potassium Channels - physiology</subject><subject>Seizure</subject><subject>Single potassium channel</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhYMoY8_oP1DIQmRclOZVqWQzIIMvGNCFrkM6dTMTSSdtkhLGX296uuilbvLgfOeSnIPQC0reUkLlO0KIHJTW_FLpN5owqQbyCG2omtggmSCP0eaEPEXntf7sV841OUNnTFEtGN-g_TdI7T5Cggat2D85whDSvDiYsbuz6RYqzh63O8A1pNsIeJ-brTUsuwc9QcQh4X0JO1vusVtiW8rBCwW2xUbscmnB9UOCpeRUn6En3sYKz9f9Av34-OH79efh5uunL9fvbwYnFG8D11ZwcFvLnRo1Vc75GYTTUhA-ypESBooo5T2RVnolmVOz305z5-zku-0CvT7O3Zf8a4HazC5UBzHaBHmpZtKcj6NU_wXpKDhjk-igOIKu5FoLeLP-2lBiDo2YQ9zmEHdfzEMjhnTby3X-st3BfDKtFXT91arb2nPyxSYX6gmTijJOWMeujhj00H4HKKa6AKn3FAq4ZuYc_v2Ovx66qk4</recordid><startdate>19890918</startdate><enddate>19890918</enddate><creator>Sugaya, Eiichi</creator><creator>Sugaya, Aiko</creator><creator>Takagi, Tamaki</creator><creator>Tsuda, Tadashi</creator><creator>Kajiwara, Kagemasa</creator><creator>Yasuda, Kazumi</creator><creator>Komatsubara, Junichi</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19890918</creationdate><title>Pentylenetetrazole-induced changes of the single potassium channel in primary cultured cerebral cortical neurons</title><author>Sugaya, Eiichi ; Sugaya, Aiko ; Takagi, Tamaki ; Tsuda, Tadashi ; Kajiwara, Kagemasa ; Yasuda, Kazumi ; Komatsubara, Junichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-39a43ecba3c85918ccfde4c96403565102e8088ff06a6f862c8dfb7dccfa7fba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Central nervous system</topic><topic>Cerebral Cortex - physiology</topic><topic>Cultured neuron</topic><topic>Electric Conductivity</topic><topic>Electrophysiology</topic><topic>Embryo, Mammalian</topic><topic>Evoked Potentials - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Neurons - drug effects</topic><topic>Neurons - physiology</topic><topic>Patch clamp</topic><topic>Pentylenetetrazole</topic><topic>Pentylenetetrazole - pharmacology</topic><topic>Potassium Channels - drug effects</topic><topic>Potassium Channels - physiology</topic><topic>Seizure</topic><topic>Single potassium channel</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugaya, Eiichi</creatorcontrib><creatorcontrib>Sugaya, Aiko</creatorcontrib><creatorcontrib>Takagi, Tamaki</creatorcontrib><creatorcontrib>Tsuda, Tadashi</creatorcontrib><creatorcontrib>Kajiwara, Kagemasa</creatorcontrib><creatorcontrib>Yasuda, Kazumi</creatorcontrib><creatorcontrib>Komatsubara, Junichi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugaya, Eiichi</au><au>Sugaya, Aiko</au><au>Takagi, Tamaki</au><au>Tsuda, Tadashi</au><au>Kajiwara, Kagemasa</au><au>Yasuda, Kazumi</au><au>Komatsubara, Junichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pentylenetetrazole-induced changes of the single potassium channel in primary cultured cerebral cortical neurons</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1989-09-18</date><risdate>1989</risdate><volume>497</volume><issue>2</issue><spage>239</spage><epage>244</epage><pages>239-244</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>To elucidate the behavior of the single ionic channels of cerebral cortical neurons during bursting activity, the effects of pentylenetetrazole (PTZ) on a single potassium channel of primary cultured cerebral cortical neurons from mice were examined. All of the examined 10-day-old primary cultured neurons of the cerebral cortex showed clear bursting activity after extracellular application of PTZ using whole-cell patch-clamp recording. Less than half of the examined single potassium channels, both outward and inward, of the 2- and 3-day-old as well as 6–10-day-old primary cultured cerebral cortical neurons showed the bursting-type open-close state by application of PTZ. The PTZ-sensitive single potassium channels were found in the voltage-dependent as well as calcium-activated channels.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2819423</pmid><doi>10.1016/0006-8993(89)90268-0</doi><tpages>6</tpages></addata></record> |
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| ispartof | Brain research, 1989-09, Vol.497 (2), p.239-244 |
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| subjects | Animals Biological and medical sciences Cells, Cultured Central nervous system Cerebral Cortex - physiology Cultured neuron Electric Conductivity Electrophysiology Embryo, Mammalian Evoked Potentials - drug effects Fundamental and applied biological sciences. Psychology Mice Mice, Inbred Strains Neurons - drug effects Neurons - physiology Patch clamp Pentylenetetrazole Pentylenetetrazole - pharmacology Potassium Channels - drug effects Potassium Channels - physiology Seizure Single potassium channel Vertebrates: nervous system and sense organs |
| title | Pentylenetetrazole-induced changes of the single potassium channel in primary cultured cerebral cortical neurons |
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