Immunohisto- and cytochemical localization of cortical nicotinic cholinoceptors in rat and man
A monoclonal antibody (WF 6) raised against purified Torpedo nicotinic acelylcholine receptor was applied to study the cellular and subcellular receptor distribution in human and rat neocortex. In both species, immunostaining was most prominent in perikarya and dendrites of the projection neurons in...
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| Veröffentlicht in: | Brain research 1989-11, Vol.502 (2), p.287-295 |
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| creator | Schröder, Hannsjörg Zilles, Karl Maelicke, Alfred Hajós, Ferenc |
| description | A monoclonal antibody (WF 6) raised against purified Torpedo nicotinic acelylcholine receptor was applied to study the cellular and subcellular receptor distribution in human and rat neocortex. In both species, immunostaining was most prominent in perikarya and dendrites of the projection neurons in layers III and V. In layer VI fusiform cells displayed immunoreactivity while in layers I, II and IV some round-shaped cells were immunostained. Subcellularly, immunoprecipitate was found in neuronal perikarya, dendrites and in the postsynaptic thickenings, indicating intracellular sites of synthesis, transport and membrane incorporation of receptor protein. The results suggest that WF 6-immunocytochemistry is a useful tool to label nicotinic cholinergic receptors rendering new information about the specific cell-type and subcellular receptor distribution hardly obtainable by using conventional receptor autoradiography. |
| doi_str_mv | 10.1016/0006-8993(89)90624-0 |
| format | Article |
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In both species, immunostaining was most prominent in perikarya and dendrites of the projection neurons in layers III and V. In layer VI fusiform cells displayed immunoreactivity while in layers I, II and IV some round-shaped cells were immunostained. Subcellularly, immunoprecipitate was found in neuronal perikarya, dendrites and in the postsynaptic thickenings, indicating intracellular sites of synthesis, transport and membrane incorporation of receptor protein. The results suggest that WF 6-immunocytochemistry is a useful tool to label nicotinic cholinergic receptors rendering new information about the specific cell-type and subcellular receptor distribution hardly obtainable by using conventional receptor autoradiography.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(89)90624-0</identifier><identifier>PMID: 2819467</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Acetylcholine ; Adult ; Aged ; Animals ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Cerebral cortex ; Cerebral Cortex - metabolism ; Cerebral Cortex - ultrastructure ; Female ; Fundamental and applied biological sciences. Psychology ; Human brain ; Humans ; Immunocytochemistry ; Immunohistochemistry ; Male ; Middle Aged ; Nicotinic acetylcholine receptor ; Rat brain ; Rats ; Rats, Inbred Strains ; Receptors, Nicotinic - metabolism ; Species Specificity ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 1989-11, Vol.502 (2), p.287-295</ispartof><rights>1989 Elsevier Science Publishers B.V. 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In both species, immunostaining was most prominent in perikarya and dendrites of the projection neurons in layers III and V. In layer VI fusiform cells displayed immunoreactivity while in layers I, II and IV some round-shaped cells were immunostained. Subcellularly, immunoprecipitate was found in neuronal perikarya, dendrites and in the postsynaptic thickenings, indicating intracellular sites of synthesis, transport and membrane incorporation of receptor protein. The results suggest that WF 6-immunocytochemistry is a useful tool to label nicotinic cholinergic receptors rendering new information about the specific cell-type and subcellular receptor distribution hardly obtainable by using conventional receptor autoradiography.</description><subject>Acetylcholine</subject><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Cerebral cortex</subject><subject>Cerebral Cortex - metabolism</subject><subject>Cerebral Cortex - ultrastructure</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human brain</subject><subject>Humans</subject><subject>Immunocytochemistry</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Nicotinic acetylcholine receptor</subject><subject>Rat brain</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Receptors, Nicotinic - metabolism</subject><subject>Species Specificity</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9r3DAQxUVISLdpvkEDviS0B7eS5ZWlSyCEtgkEcmmvFePxmFWxpa2kLaSfvto_JLfm8obhvXkMP8beC_5JcKE-c85VrY2RH7T5aLhq2pofsYXQXVOXhR-zxXPkDXub0q-ySmn4KTtttDCt6hbs5_08b3xYuZRDXYEfKnzKAVc0O4SpmkJR9xeyC74KY4Uh5p3hHYbsila4CpPzAWmdQ0yV81WEvGuawb9jJyNMic4P84z9-Prl--1d_fD47f725qFGKWWuAdpRIPZi5AKkQqCmFzB0qhFmqUdpQGrNR24EcE5SadH2UnNSPfWIRPKMXe171zH83lDKdnYJaZrAU9gk2xkp21abV4Ni2apl1zQl2O6DGENKkUa7jm6G-GQFt1v-dgvXbuEWsTv-lpezi0P_pp9peD46AC_-5cGHVDiOETy69NJttk82uuSu9zkq1P44ijahI480uEiY7RDc_x_5B39coro</recordid><startdate>19891120</startdate><enddate>19891120</enddate><creator>Schröder, Hannsjörg</creator><creator>Zilles, Karl</creator><creator>Maelicke, Alfred</creator><creator>Hajós, Ferenc</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19891120</creationdate><title>Immunohisto- and cytochemical localization of cortical nicotinic cholinoceptors in rat and man</title><author>Schröder, Hannsjörg ; Zilles, Karl ; Maelicke, Alfred ; Hajós, Ferenc</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c333t-aa4f1ccb1f01a36cae2b1ad7621958f39a3880f091a00e36814b380e6bebccee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Acetylcholine</topic><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Cerebral cortex</topic><topic>Cerebral Cortex - metabolism</topic><topic>Cerebral Cortex - ultrastructure</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human brain</topic><topic>Humans</topic><topic>Immunocytochemistry</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Nicotinic acetylcholine receptor</topic><topic>Rat brain</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Receptors, Nicotinic - metabolism</topic><topic>Species Specificity</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schröder, Hannsjörg</creatorcontrib><creatorcontrib>Zilles, Karl</creatorcontrib><creatorcontrib>Maelicke, Alfred</creatorcontrib><creatorcontrib>Hajós, Ferenc</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schröder, Hannsjörg</au><au>Zilles, Karl</au><au>Maelicke, Alfred</au><au>Hajós, Ferenc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohisto- and cytochemical localization of cortical nicotinic cholinoceptors in rat and man</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1989-11-20</date><risdate>1989</risdate><volume>502</volume><issue>2</issue><spage>287</spage><epage>295</epage><pages>287-295</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>A monoclonal antibody (WF 6) raised against purified Torpedo nicotinic acelylcholine receptor was applied to study the cellular and subcellular receptor distribution in human and rat neocortex. In both species, immunostaining was most prominent in perikarya and dendrites of the projection neurons in layers III and V. In layer VI fusiform cells displayed immunoreactivity while in layers I, II and IV some round-shaped cells were immunostained. Subcellularly, immunoprecipitate was found in neuronal perikarya, dendrites and in the postsynaptic thickenings, indicating intracellular sites of synthesis, transport and membrane incorporation of receptor protein. The results suggest that WF 6-immunocytochemistry is a useful tool to label nicotinic cholinergic receptors rendering new information about the specific cell-type and subcellular receptor distribution hardly obtainable by using conventional receptor autoradiography.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2819467</pmid><doi>10.1016/0006-8993(89)90624-0</doi><tpages>9</tpages></addata></record> |
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| subjects | Acetylcholine Adult Aged Animals Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Cerebral cortex Cerebral Cortex - metabolism Cerebral Cortex - ultrastructure Female Fundamental and applied biological sciences. Psychology Human brain Humans Immunocytochemistry Immunohistochemistry Male Middle Aged Nicotinic acetylcholine receptor Rat brain Rats Rats, Inbred Strains Receptors, Nicotinic - metabolism Species Specificity Vertebrates: nervous system and sense organs |
| title | Immunohisto- and cytochemical localization of cortical nicotinic cholinoceptors in rat and man |
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