Gastritis in urease-immunized mice after Helicobacter felis challenge may be due to residual bacteria
BACKGROUND & AIMS: Oral immunization with recombinant Helicobacter pylori urease (rUre) coadministered with a mucosal adjuvant protects mice against challenge with Helicobacter felis. In this study, the duration of protection and gastritis after challenge were characterized at sequential time in...
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| Veröffentlicht in: | Gastroenterology (New York, N.Y. 1943) N.Y. 1943), 1997-10, Vol.113 (4), p.1118-1128 |
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| container_title | Gastroenterology (New York, N.Y. 1943) |
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| creator | Ermak, TH Ding, R Ekstein, B Hill, J Myers, GA Lee, CK Pappo, J Kleanthous, HK Monath, TP |
| description | BACKGROUND & AIMS: Oral immunization with recombinant Helicobacter pylori urease (rUre) coadministered with a mucosal adjuvant protects mice against challenge with Helicobacter felis. In this study, the duration of protection and gastritis after challenge were characterized at sequential time intervals up to 1 year.
METHODS: Outbred Swiss- Webster mice were orally immunized with rUre plus adjuvant and examined for the presence of H. felis infection and leukocyte infiltration into the gastric mucosa.
RESULTS: When defined by gastric urease activity, 70%-95% of rUre-immunized mice were protected for between 2 and 57 weeks. Challenge with H. felis increased the inflammatory response in the gastric mucosa of rUre-immunized mice, which also had elevated CD4+ and CD8+ T cells. The CD8+ cells represented a population of gastric intraepithelial cells, which expressed the mucosal alpha E-integrin. Epithelial changes consisting of parietal cell loss and hyperplasia of the epithelium occurred in approximately 20% of the mice. Antimicrobial triple therapy significantly decreased the degree of gastritis and epithelial alteration in the stomach.
CONCLUSIONS: These results indicate that oral immunization of mice with rUre produces a long- lasting inhibition of H. felis infection but that residual bacteria may produce a persistent lymphocytic infiltration under these experimental conditions.
(Gastroenterology 1997 Oct;113(4):1118-28) |
| doi_str_mv | 10.1053/gast.1997.v113.pm9322506 |
| format | Article |
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METHODS: Outbred Swiss- Webster mice were orally immunized with rUre plus adjuvant and examined for the presence of H. felis infection and leukocyte infiltration into the gastric mucosa.
RESULTS: When defined by gastric urease activity, 70%-95% of rUre-immunized mice were protected for between 2 and 57 weeks. Challenge with H. felis increased the inflammatory response in the gastric mucosa of rUre-immunized mice, which also had elevated CD4+ and CD8+ T cells. The CD8+ cells represented a population of gastric intraepithelial cells, which expressed the mucosal alpha E-integrin. Epithelial changes consisting of parietal cell loss and hyperplasia of the epithelium occurred in approximately 20% of the mice. Antimicrobial triple therapy significantly decreased the degree of gastritis and epithelial alteration in the stomach.
CONCLUSIONS: These results indicate that oral immunization of mice with rUre produces a long- lasting inhibition of H. felis infection but that residual bacteria may produce a persistent lymphocytic infiltration under these experimental conditions.
(Gastroenterology 1997 Oct;113(4):1118-28)</description><identifier>ISSN: 0016-5085</identifier><identifier>EISSN: 1528-0012</identifier><identifier>DOI: 10.1053/gast.1997.v113.pm9322506</identifier><identifier>PMID: 9322506</identifier><identifier>CODEN: GASTAB</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animal bacterial diseases ; Animals ; Bacterial diseases ; Bacterial Vaccines ; Biological and medical sciences ; Female ; Flow Cytometry ; Gastric Mucosa - immunology ; Gastric Mucosa - microbiology ; Gastric Mucosa - pathology ; Gastritis - microbiology ; Helicobacter - enzymology ; Helicobacter - isolation & purification ; Helicobacter Infections - complications ; Helicobacter Infections - immunology ; Helicobacter pylori - enzymology ; Helicobacter pylori - immunology ; Immunophenotyping ; Infectious diseases ; Integrins - biosynthesis ; Intestinal Mucosa - immunology ; Intestinal Mucosa - microbiology ; Intestinal Mucosa - pathology ; Medical sciences ; Metaplasia ; Mice ; Receptors, Antigen, T-Cell, alpha-beta - biosynthesis ; T-Lymphocyte Subsets - immunology ; T-Lymphocyte Subsets - pathology ; Urease - analysis ; Urease - immunology ; Vaccines, Synthetic</subject><ispartof>Gastroenterology (New York, N.Y. 1943), 1997-10, Vol.113 (4), p.1118-1128</ispartof><rights>1997</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-c89407fb60e7f9a13da5bab934a882a553762325d1ef9b7e3556018f9fbf084b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0016508597004630$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2834227$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9322506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ermak, TH</creatorcontrib><creatorcontrib>Ding, R</creatorcontrib><creatorcontrib>Ekstein, B</creatorcontrib><creatorcontrib>Hill, J</creatorcontrib><creatorcontrib>Myers, GA</creatorcontrib><creatorcontrib>Lee, CK</creatorcontrib><creatorcontrib>Pappo, J</creatorcontrib><creatorcontrib>Kleanthous, HK</creatorcontrib><creatorcontrib>Monath, TP</creatorcontrib><title>Gastritis in urease-immunized mice after Helicobacter felis challenge may be due to residual bacteria</title><title>Gastroenterology (New York, N.Y. 1943)</title><addtitle>Gastroenterology</addtitle><description>BACKGROUND & AIMS: Oral immunization with recombinant Helicobacter pylori urease (rUre) coadministered with a mucosal adjuvant protects mice against challenge with Helicobacter felis. In this study, the duration of protection and gastritis after challenge were characterized at sequential time intervals up to 1 year.
METHODS: Outbred Swiss- Webster mice were orally immunized with rUre plus adjuvant and examined for the presence of H. felis infection and leukocyte infiltration into the gastric mucosa.
RESULTS: When defined by gastric urease activity, 70%-95% of rUre-immunized mice were protected for between 2 and 57 weeks. Challenge with H. felis increased the inflammatory response in the gastric mucosa of rUre-immunized mice, which also had elevated CD4+ and CD8+ T cells. The CD8+ cells represented a population of gastric intraepithelial cells, which expressed the mucosal alpha E-integrin. Epithelial changes consisting of parietal cell loss and hyperplasia of the epithelium occurred in approximately 20% of the mice. Antimicrobial triple therapy significantly decreased the degree of gastritis and epithelial alteration in the stomach.
CONCLUSIONS: These results indicate that oral immunization of mice with rUre produces a long- lasting inhibition of H. felis infection but that residual bacteria may produce a persistent lymphocytic infiltration under these experimental conditions.
(Gastroenterology 1997 Oct;113(4):1118-28)</description><subject>Animal bacterial diseases</subject><subject>Animals</subject><subject>Bacterial diseases</subject><subject>Bacterial Vaccines</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Gastric Mucosa - immunology</subject><subject>Gastric Mucosa - microbiology</subject><subject>Gastric Mucosa - pathology</subject><subject>Gastritis - microbiology</subject><subject>Helicobacter - enzymology</subject><subject>Helicobacter - isolation & purification</subject><subject>Helicobacter Infections - complications</subject><subject>Helicobacter Infections - immunology</subject><subject>Helicobacter pylori - enzymology</subject><subject>Helicobacter pylori - immunology</subject><subject>Immunophenotyping</subject><subject>Infectious diseases</subject><subject>Integrins - biosynthesis</subject><subject>Intestinal Mucosa - immunology</subject><subject>Intestinal Mucosa - microbiology</subject><subject>Intestinal Mucosa - pathology</subject><subject>Medical sciences</subject><subject>Metaplasia</subject><subject>Mice</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - biosynthesis</subject><subject>T-Lymphocyte Subsets - immunology</subject><subject>T-Lymphocyte Subsets - pathology</subject><subject>Urease - analysis</subject><subject>Urease - immunology</subject><subject>Vaccines, Synthetic</subject><issn>0016-5085</issn><issn>1528-0012</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLJDEQgIOs6Pj4CUIOi7eezaPTnRxX0XFB2Mt6DpV0RSP9GJNuQX-93cwwHvdUFPXV6yOEcrbmTMlfz5DHNTemXr9zLtfbzkghFKuOyIoroQvGuPhBVnOoCsW0OiVnOb8yxozU_ISc7PEVwc08KcUxZhp7OiWEjEXsuqmPn9jQLnqkEEZM9AHb6AcHfknCnGTqX6BtsX9G2sEHdUibCek40IQ5NhO0dEdHuCDHAdqMl_t4Tp7u7_7dPhSPfzd_bn8_Fr4s9Vh4bUpWB1cxrIMBLhtQDpyRJWgtQClZV0IK1XAMxtUolaoY18EEF5gunTwn17u52zS8TZhH28XssW2hx2HKtp7_rlhlZlDvQJ-GnBMGu02xg_RhObOLYbsYtothuxi2B8Nz69V-x-Q6bA6N3_Wf-zpkD21I0PuYD5jQshSinrGbHYazj_eIyWYfsffYxIR-tM0Q_3_LF4tbnSM</recordid><startdate>19971001</startdate><enddate>19971001</enddate><creator>Ermak, TH</creator><creator>Ding, R</creator><creator>Ekstein, B</creator><creator>Hill, J</creator><creator>Myers, GA</creator><creator>Lee, CK</creator><creator>Pappo, J</creator><creator>Kleanthous, HK</creator><creator>Monath, TP</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19971001</creationdate><title>Gastritis in urease-immunized mice after Helicobacter felis challenge may be due to residual bacteria</title><author>Ermak, TH ; Ding, R ; Ekstein, B ; Hill, J ; Myers, GA ; Lee, CK ; Pappo, J ; Kleanthous, HK ; Monath, TP</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-c89407fb60e7f9a13da5bab934a882a553762325d1ef9b7e3556018f9fbf084b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animal bacterial diseases</topic><topic>Animals</topic><topic>Bacterial diseases</topic><topic>Bacterial Vaccines</topic><topic>Biological and medical sciences</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Gastric Mucosa - immunology</topic><topic>Gastric Mucosa - microbiology</topic><topic>Gastric Mucosa - pathology</topic><topic>Gastritis - microbiology</topic><topic>Helicobacter - enzymology</topic><topic>Helicobacter - isolation & purification</topic><topic>Helicobacter Infections - complications</topic><topic>Helicobacter Infections - immunology</topic><topic>Helicobacter pylori - enzymology</topic><topic>Helicobacter pylori - immunology</topic><topic>Immunophenotyping</topic><topic>Infectious diseases</topic><topic>Integrins - biosynthesis</topic><topic>Intestinal Mucosa - immunology</topic><topic>Intestinal Mucosa - microbiology</topic><topic>Intestinal Mucosa - pathology</topic><topic>Medical sciences</topic><topic>Metaplasia</topic><topic>Mice</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - biosynthesis</topic><topic>T-Lymphocyte Subsets - immunology</topic><topic>T-Lymphocyte Subsets - pathology</topic><topic>Urease - analysis</topic><topic>Urease - immunology</topic><topic>Vaccines, Synthetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ermak, TH</creatorcontrib><creatorcontrib>Ding, R</creatorcontrib><creatorcontrib>Ekstein, B</creatorcontrib><creatorcontrib>Hill, J</creatorcontrib><creatorcontrib>Myers, GA</creatorcontrib><creatorcontrib>Lee, CK</creatorcontrib><creatorcontrib>Pappo, J</creatorcontrib><creatorcontrib>Kleanthous, HK</creatorcontrib><creatorcontrib>Monath, TP</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ermak, TH</au><au>Ding, R</au><au>Ekstein, B</au><au>Hill, J</au><au>Myers, GA</au><au>Lee, CK</au><au>Pappo, J</au><au>Kleanthous, HK</au><au>Monath, TP</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gastritis in urease-immunized mice after Helicobacter felis challenge may be due to residual bacteria</atitle><jtitle>Gastroenterology (New York, N.Y. 1943)</jtitle><addtitle>Gastroenterology</addtitle><date>1997-10-01</date><risdate>1997</risdate><volume>113</volume><issue>4</issue><spage>1118</spage><epage>1128</epage><pages>1118-1128</pages><issn>0016-5085</issn><eissn>1528-0012</eissn><coden>GASTAB</coden><abstract>BACKGROUND & AIMS: Oral immunization with recombinant Helicobacter pylori urease (rUre) coadministered with a mucosal adjuvant protects mice against challenge with Helicobacter felis. In this study, the duration of protection and gastritis after challenge were characterized at sequential time intervals up to 1 year.
METHODS: Outbred Swiss- Webster mice were orally immunized with rUre plus adjuvant and examined for the presence of H. felis infection and leukocyte infiltration into the gastric mucosa.
RESULTS: When defined by gastric urease activity, 70%-95% of rUre-immunized mice were protected for between 2 and 57 weeks. Challenge with H. felis increased the inflammatory response in the gastric mucosa of rUre-immunized mice, which also had elevated CD4+ and CD8+ T cells. The CD8+ cells represented a population of gastric intraepithelial cells, which expressed the mucosal alpha E-integrin. Epithelial changes consisting of parietal cell loss and hyperplasia of the epithelium occurred in approximately 20% of the mice. Antimicrobial triple therapy significantly decreased the degree of gastritis and epithelial alteration in the stomach.
CONCLUSIONS: These results indicate that oral immunization of mice with rUre produces a long- lasting inhibition of H. felis infection but that residual bacteria may produce a persistent lymphocytic infiltration under these experimental conditions.
(Gastroenterology 1997 Oct;113(4):1118-28)</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>9322506</pmid><doi>10.1053/gast.1997.v113.pm9322506</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Gastroenterology (New York, N.Y. 1943), 1997-10, Vol.113 (4), p.1118-1128 |
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| subjects | Animal bacterial diseases Animals Bacterial diseases Bacterial Vaccines Biological and medical sciences Female Flow Cytometry Gastric Mucosa - immunology Gastric Mucosa - microbiology Gastric Mucosa - pathology Gastritis - microbiology Helicobacter - enzymology Helicobacter - isolation & purification Helicobacter Infections - complications Helicobacter Infections - immunology Helicobacter pylori - enzymology Helicobacter pylori - immunology Immunophenotyping Infectious diseases Integrins - biosynthesis Intestinal Mucosa - immunology Intestinal Mucosa - microbiology Intestinal Mucosa - pathology Medical sciences Metaplasia Mice Receptors, Antigen, T-Cell, alpha-beta - biosynthesis T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - pathology Urease - analysis Urease - immunology Vaccines, Synthetic |
| title | Gastritis in urease-immunized mice after Helicobacter felis challenge may be due to residual bacteria |
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