Bioluminescent assays using glucose-6-phosphate dehydrogenase: Application to biotin and streptavidin detection

A streptavidin-glucose-6-phosphate dehydrogenase (G6PDH) conjugate was synthesized and its properties were studied, along with those of biotin-G6PDH conjugates. Two bioluminescent assays were used. Streptavidin was assayed in two steps: streptavidin samples were first incubated with a small amount o...

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Veröffentlicht in:	Analytical biochemistry 1989-07, Vol.180 (1), p.43-49
Hauptverfasser:	Térouanne, Béatrice, Bencheick, Myriem, Balaguer, Patrick, Boussioux, Anne-Marie, Nicolas, Jean-Claude
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Animals Bacterial Proteins - analysis Biological and medical sciences bioluminescence Biotechnology biotin Biotin - analysis Collodion Cross-Linking Reagents DNA Fundamental and applied biological sciences. Psychology gamma-Globulins Glucosephosphate Dehydrogenase Luminescent Measurements Maleimides Methods. Procedures. Technologies Others Rabbits Streptavidin Sulfhydryl Compounds Various methods and equipments
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