Down-Regulated Expression of Perforin-Positive/CD16+ Cells in the Peripheral Blood Lymphocytes in the First Trimester of Pregnancy and Up-Regulation at the End of Pregnancy
PROBLEM: Immunophenotypic profiles of perforin (P)‐positive peripheral blood lymphocytes in the first trimester and at the term of human pregnancy were analyzed. METHOD OF STUDY: Perforin expression in peripheral blood lymphocyte subsets was measured by simultaneous detection of P (intracellular ant...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 1997-09, Vol.38 (3), p.189-196 |
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| container_title | American journal of reproductive immunology (1989) |
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| creator | Rukavina, Daniel Podack, Eckhard R. Rubesa, Gordana Spanjol-Pandelo, Suzana Randic, Ljiljana |
| description | PROBLEM: Immunophenotypic profiles of perforin (P)‐positive peripheral blood lymphocytes in the first trimester and at the term of human pregnancy were analyzed.
METHOD OF STUDY: Perforin expression in peripheral blood lymphocyte subsets was measured by simultaneous detection of P (intracellular antigen) and cell‐surface antigens (CD3, CD4, CD8, CD16, and CD56) by flow cytometry in nonpregnant (NP) and pregnant women in the first trimester (FTP) and at the time of parturition (TP).
RESULTS: The percentage of total P+ cells in peripheral blood compared to nonpregnant women was slightly lower in the FTP but significantly higher at TP. Perforin‐positive cells were significantly elevated in T lymphocyte subsets (CD3+P+, CD4+P\ CD8+P+) in both the FTP and TP groups, as was the percentage of CD56+P+ cells. Profound changes in the CD16+ subpopulation were found in the FTP group compared to both the NP and TP groups (a drastic decrease of CD16+P+ cells; CD16+ cells among P+ cells; P+ cells among CD16+ cells). A considerable part of CD3+ cells in both the FTP and TP groups are CD3+CD56+P+. The average fluorescence intensity (AFI) for P (a measure of P content per cell) was significantly decreased in FTP and increased in TP groups.
CONCLUSIONS: The CD16 molecule is FcγRIIIA which is the only Fc receptor responsible for antibody dependent cell‐cytotoxicity (ADCC) of NK and T‐cells. In the first‐trimester human pregnancy this mechanism is severely down‐regulated compared to both the NP and TP groups. |
| doi_str_mv | 10.1111/j.1600-0897.1997.tb00297.x |
| format | Article |
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METHOD OF STUDY: Perforin expression in peripheral blood lymphocyte subsets was measured by simultaneous detection of P (intracellular antigen) and cell‐surface antigens (CD3, CD4, CD8, CD16, and CD56) by flow cytometry in nonpregnant (NP) and pregnant women in the first trimester (FTP) and at the time of parturition (TP).
RESULTS: The percentage of total P+ cells in peripheral blood compared to nonpregnant women was slightly lower in the FTP but significantly higher at TP. Perforin‐positive cells were significantly elevated in T lymphocyte subsets (CD3+P+, CD4+P\ CD8+P+) in both the FTP and TP groups, as was the percentage of CD56+P+ cells. Profound changes in the CD16+ subpopulation were found in the FTP group compared to both the NP and TP groups (a drastic decrease of CD16+P+ cells; CD16+ cells among P+ cells; P+ cells among CD16+ cells). A considerable part of CD3+ cells in both the FTP and TP groups are CD3+CD56+P+. The average fluorescence intensity (AFI) for P (a measure of P content per cell) was significantly decreased in FTP and increased in TP groups.
CONCLUSIONS: The CD16 molecule is FcγRIIIA which is the only Fc receptor responsible for antibody dependent cell‐cytotoxicity (ADCC) of NK and T‐cells. In the first‐trimester human pregnancy this mechanism is severely down‐regulated compared to both the NP and TP groups.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/j.1600-0897.1997.tb00297.x</identifier><identifier>PMID: 9325491</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Antibodies, Monoclonal ; Biological and medical sciences ; CD56 Antigen - blood ; Down-Regulation ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Hormone metabolism and regulation ; Humans ; Immunobiology ; Killer Cells, Natural - immunology ; Killer Cells, Natural - metabolism ; Labor, Obstetric - blood ; Labor, Obstetric - immunology ; Lymphocyte Subsets - immunology ; Lymphocyte Subsets - metabolism ; Lymphocytes ; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation ; Maternal-Fetal Exchange - immunology ; Membrane Glycoproteins - blood ; Mice ; NK cells ; Perforin ; peripheral blood ; Phenotype ; Pore Forming Cytotoxic Proteins ; pregnancy ; Pregnancy - blood ; Pregnancy - immunology ; Pregnancy Trimester, First ; Pregnancy. Parturition. Lactation ; Receptors, IgG - blood ; T lymphocytes ; T-Lymphocyte Subsets - immunology ; T-Lymphocyte Subsets - metabolism ; Up-Regulation ; Vertebrates: reproduction</subject><ispartof>American journal of reproductive immunology (1989), 1997-09, Vol.38 (3), p.189-196</ispartof><rights>1997 Munksgaard</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4367-229ccf318b362050e25fd662dc6ae3734c92ee1258850295721232c9cf9394603</citedby><cites>FETCH-LOGICAL-c4367-229ccf318b362050e25fd662dc6ae3734c92ee1258850295721232c9cf9394603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0897.1997.tb00297.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0897.1997.tb00297.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>310,311,315,782,786,791,792,1419,23937,23938,25147,27931,27932,45581,45582</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2830993$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9325491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rukavina, Daniel</creatorcontrib><creatorcontrib>Podack, Eckhard R.</creatorcontrib><creatorcontrib>Rubesa, Gordana</creatorcontrib><creatorcontrib>Spanjol-Pandelo, Suzana</creatorcontrib><creatorcontrib>Randic, Ljiljana</creatorcontrib><title>Down-Regulated Expression of Perforin-Positive/CD16+ Cells in the Peripheral Blood Lymphocytes in the First Trimester of Pregnancy and Up-Regulation at the End of Pregnancy</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>PROBLEM: Immunophenotypic profiles of perforin (P)‐positive peripheral blood lymphocytes in the first trimester and at the term of human pregnancy were analyzed.
METHOD OF STUDY: Perforin expression in peripheral blood lymphocyte subsets was measured by simultaneous detection of P (intracellular antigen) and cell‐surface antigens (CD3, CD4, CD8, CD16, and CD56) by flow cytometry in nonpregnant (NP) and pregnant women in the first trimester (FTP) and at the time of parturition (TP).
RESULTS: The percentage of total P+ cells in peripheral blood compared to nonpregnant women was slightly lower in the FTP but significantly higher at TP. Perforin‐positive cells were significantly elevated in T lymphocyte subsets (CD3+P+, CD4+P\ CD8+P+) in both the FTP and TP groups, as was the percentage of CD56+P+ cells. Profound changes in the CD16+ subpopulation were found in the FTP group compared to both the NP and TP groups (a drastic decrease of CD16+P+ cells; CD16+ cells among P+ cells; P+ cells among CD16+ cells). A considerable part of CD3+ cells in both the FTP and TP groups are CD3+CD56+P+. The average fluorescence intensity (AFI) for P (a measure of P content per cell) was significantly decreased in FTP and increased in TP groups.
CONCLUSIONS: The CD16 molecule is FcγRIIIA which is the only Fc receptor responsible for antibody dependent cell‐cytotoxicity (ADCC) of NK and T‐cells. In the first‐trimester human pregnancy this mechanism is severely down‐regulated compared to both the NP and TP groups.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>CD56 Antigen - blood</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Hormone metabolism and regulation</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Killer Cells, Natural - immunology</subject><subject>Killer Cells, Natural - metabolism</subject><subject>Labor, Obstetric - blood</subject><subject>Labor, Obstetric - immunology</subject><subject>Lymphocyte Subsets - immunology</subject><subject>Lymphocyte Subsets - metabolism</subject><subject>Lymphocytes</subject><subject>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</subject><subject>Maternal-Fetal Exchange - immunology</subject><subject>Membrane Glycoproteins - blood</subject><subject>Mice</subject><subject>NK cells</subject><subject>Perforin</subject><subject>peripheral blood</subject><subject>Phenotype</subject><subject>Pore Forming Cytotoxic Proteins</subject><subject>pregnancy</subject><subject>Pregnancy - blood</subject><subject>Pregnancy - immunology</subject><subject>Pregnancy Trimester, First</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Receptors, IgG - blood</subject><subject>T lymphocytes</subject><subject>T-Lymphocyte Subsets - immunology</subject><subject>T-Lymphocyte Subsets - metabolism</subject><subject>Up-Regulation</subject><subject>Vertebrates: reproduction</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUV1v0zAUjRBobIOfgGQhxAtK548kjnlA2rJ2DFUwwcYeLde5Wd2lcbDTrflP_EictUS84gf7yufccz9OFL0leELCOVlNSIZxjHPBJ0SEq1tgTMO7fRYdjtDzEOMki3mC85fRkfcrjMM_4wfRgWA0TQQ5jH6f28cm_g53m1p1UKLptnXgvbENshW6AldZZ5r4ynrTmQc4Kc5J9gEVUNcemQZ1SxhIpl2CUzU6q60t0bxft0ur-w5Gzsw436FrZ9bgO3BP2g7uGtXoHqmmRDft3yaG0qp7ypoG4F_mq-hFpWoPr_fvcXQzm14Xn-P5t4vL4nQe64RlPKZUaF0xki9YRnGKgaZVmWW01JkCxlmiBQUgNM3zNKwt5ZRQRrXQlWAiyTA7jt7vdFtnf21Cx3JtvA4zqwbsxkse1kdpwgPx446onfXeQSXbMKJyvSRYDlbJlRz8kIMfcrBK7q2S25D8Zl9ls1hDOabuvQn4uz2uvFZ15cIKjB9pNGdYCBZon3a0R1ND_x8NyNMvlyEIAvFOwARrtqOAcvcy44yn8vbrhfwxS4sz8lPIW_YHpMHALA</recordid><startdate>199709</startdate><enddate>199709</enddate><creator>Rukavina, Daniel</creator><creator>Podack, Eckhard R.</creator><creator>Rubesa, Gordana</creator><creator>Spanjol-Pandelo, Suzana</creator><creator>Randic, Ljiljana</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199709</creationdate><title>Down-Regulated Expression of Perforin-Positive/CD16+ Cells in the Peripheral Blood Lymphocytes in the First Trimester of Pregnancy and Up-Regulation at the End of Pregnancy</title><author>Rukavina, Daniel ; Podack, Eckhard R. ; Rubesa, Gordana ; Spanjol-Pandelo, Suzana ; Randic, Ljiljana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4367-229ccf318b362050e25fd662dc6ae3734c92ee1258850295721232c9cf9394603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>CD56 Antigen - blood</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Hormone metabolism and regulation</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Killer Cells, Natural - immunology</topic><topic>Killer Cells, Natural - metabolism</topic><topic>Labor, Obstetric - blood</topic><topic>Labor, Obstetric - immunology</topic><topic>Lymphocyte Subsets - immunology</topic><topic>Lymphocyte Subsets - metabolism</topic><topic>Lymphocytes</topic><topic>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</topic><topic>Maternal-Fetal Exchange - immunology</topic><topic>Membrane Glycoproteins - blood</topic><topic>Mice</topic><topic>NK cells</topic><topic>Perforin</topic><topic>peripheral blood</topic><topic>Phenotype</topic><topic>Pore Forming Cytotoxic Proteins</topic><topic>pregnancy</topic><topic>Pregnancy - blood</topic><topic>Pregnancy - immunology</topic><topic>Pregnancy Trimester, First</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Receptors, IgG - blood</topic><topic>T lymphocytes</topic><topic>T-Lymphocyte Subsets - immunology</topic><topic>T-Lymphocyte Subsets - metabolism</topic><topic>Up-Regulation</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rukavina, Daniel</creatorcontrib><creatorcontrib>Podack, Eckhard R.</creatorcontrib><creatorcontrib>Rubesa, Gordana</creatorcontrib><creatorcontrib>Spanjol-Pandelo, Suzana</creatorcontrib><creatorcontrib>Randic, Ljiljana</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rukavina, Daniel</au><au>Podack, Eckhard R.</au><au>Rubesa, Gordana</au><au>Spanjol-Pandelo, Suzana</au><au>Randic, Ljiljana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Down-Regulated Expression of Perforin-Positive/CD16+ Cells in the Peripheral Blood Lymphocytes in the First Trimester of Pregnancy and Up-Regulation at the End of Pregnancy</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>1997-09</date><risdate>1997</risdate><volume>38</volume><issue>3</issue><spage>189</spage><epage>196</epage><pages>189-196</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>PROBLEM: Immunophenotypic profiles of perforin (P)‐positive peripheral blood lymphocytes in the first trimester and at the term of human pregnancy were analyzed.
METHOD OF STUDY: Perforin expression in peripheral blood lymphocyte subsets was measured by simultaneous detection of P (intracellular antigen) and cell‐surface antigens (CD3, CD4, CD8, CD16, and CD56) by flow cytometry in nonpregnant (NP) and pregnant women in the first trimester (FTP) and at the time of parturition (TP).
RESULTS: The percentage of total P+ cells in peripheral blood compared to nonpregnant women was slightly lower in the FTP but significantly higher at TP. Perforin‐positive cells were significantly elevated in T lymphocyte subsets (CD3+P+, CD4+P\ CD8+P+) in both the FTP and TP groups, as was the percentage of CD56+P+ cells. Profound changes in the CD16+ subpopulation were found in the FTP group compared to both the NP and TP groups (a drastic decrease of CD16+P+ cells; CD16+ cells among P+ cells; P+ cells among CD16+ cells). A considerable part of CD3+ cells in both the FTP and TP groups are CD3+CD56+P+. The average fluorescence intensity (AFI) for P (a measure of P content per cell) was significantly decreased in FTP and increased in TP groups.
CONCLUSIONS: The CD16 molecule is FcγRIIIA which is the only Fc receptor responsible for antibody dependent cell‐cytotoxicity (ADCC) of NK and T‐cells. In the first‐trimester human pregnancy this mechanism is severely down‐regulated compared to both the NP and TP groups.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9325491</pmid><doi>10.1111/j.1600-0897.1997.tb00297.x</doi><tpages>8</tpages></addata></record> |
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| ispartof | American journal of reproductive immunology (1989), 1997-09, Vol.38 (3), p.189-196 |
| issn | 1046-7408 1600-0897 |
| language | eng |
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| subjects | Animals Antibodies, Monoclonal Biological and medical sciences CD56 Antigen - blood Down-Regulation Female Fundamental and applied biological sciences. Psychology Fundamental immunology Hormone metabolism and regulation Humans Immunobiology Killer Cells, Natural - immunology Killer Cells, Natural - metabolism Labor, Obstetric - blood Labor, Obstetric - immunology Lymphocyte Subsets - immunology Lymphocyte Subsets - metabolism Lymphocytes Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation Maternal-Fetal Exchange - immunology Membrane Glycoproteins - blood Mice NK cells Perforin peripheral blood Phenotype Pore Forming Cytotoxic Proteins pregnancy Pregnancy - blood Pregnancy - immunology Pregnancy Trimester, First Pregnancy. Parturition. Lactation Receptors, IgG - blood T lymphocytes T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - metabolism Up-Regulation Vertebrates: reproduction |
| title | Down-Regulated Expression of Perforin-Positive/CD16+ Cells in the Peripheral Blood Lymphocytes in the First Trimester of Pregnancy and Up-Regulation at the End of Pregnancy |
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