A Procedure of Silver Staining for Nucleic Acids in Agarose Gels without Pretreatment or Drying Steps

In this paper, we report a fast, simple, and reproducible staining protocol for nucleic acids in agarose gels with a sensitivity in the order of 10 pg/mm2. It took only three steps: fixation, incubation with silver ions, and development of the gels (total time 50 min). The resulting calibration curv...

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Veröffentlicht in:	Analytical biochemistry 1997-10, Vol.252 (1), p.15-18
Hauptverfasser:	Prieto, Claudio C., Leonardelli, Raúl I., Zalazar, Fabián E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage lambda - genetics Densitometry - methods Deoxyribonuclease HindIII - genetics Deoxyribonuclease HindIII - metabolism DNA - chemistry DNA - metabolism Electrophoresis, Agar Gel - methods Ethidium - chemistry Fluorescent Dyes - chemistry Nucleic Acids - chemistry Sensitivity and Specificity Silver Staining - methods
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