5-hydroxytryptamine receptor subtype messenger RNAs in human dorsal root ganglia: a polymerase chain reaction study
Although serotonin has been shown to play an important role in peripheral pain mechanisms, the specific subtypes of serotonin receptors involved in pain and hyperalgesia remain poorly understood. To date, no previous study has attempted to determine the presence of any serotonin receptor subtype in...
Gespeichert in:
| Veröffentlicht in: | Neuroscience 1997-12, Vol.81 (3), p.813-819 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 819 |
|---|---|
| container_issue | 3 |
| container_start_page | 813 |
| container_title | Neuroscience |
| container_volume | 81 |
| creator | Pierce, P.A Xie, G.-X Meuser, T Peroutka, S.J |
| description | Although serotonin has been shown to play an important role in peripheral pain mechanisms, the specific subtypes of serotonin receptors involved in pain and hyperalgesia remain poorly understood. To date, no previous study has attempted to determine the presence of any serotonin receptor subtype in human dorsal root ganglia. In this study, the presence of messenger RNA for eight human serotonin receptor subtypes in lumbar dorsal root ganglia was detected using the method of polymerase chain reaction. Dorsal root ganglia were excised
post mortem from four patients. Oligonucleotide primers were chosen based on unique regions of complimentary DNA sequence for eight cloned human serotonin receptor subtypes (i.e. 5-HT
1A, 5-HT
1D
α
, 5-HT
1D
β
, 5-HT
1E, 5-HT
1F, 5-HT
2A, 5-HT
2C and 5-HT
7). The presence of 5-HT
1D
α
, 5-HT
1D
β
, 5-HT
1E, 5-HT
1F, 5-HT
2A and 5-HT
7 receptor subtype messenger RNA was detected in dorsal root ganglia from three of the four subjects. 5-HT
1A receptor subtype messenger RNA was detected in one of the four subjects. No 5-HT
2C receptor subtype messenger RNA could be detected.
Findings from this study may direct further efforts to determine the role of serotonin receptors in the peripheral nervous system. |
| doi_str_mv | 10.1016/S0306-4522(97)00235-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79318341</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0306452297002352</els_id><sourcerecordid>16464805</sourcerecordid><originalsourceid>FETCH-LOGICAL-c420t-6a7209ae6b3a1c15a3d3fcd3aa647cc35d3b1938e0b12a97abfbb827873684fd3</originalsourceid><addsrcrecordid>eNqFkUlvFDEQhS0ECkPgJ0TyASE4NPHWG5coilgiRUFiOVvVdvWMUXe7sd0R_e_xZEZzjS91eF-Vq94j5IKzj5zx6vInk6wqVCnE-7b-wJiQZSGekQ1valnUpVLPyeaEvCSvYvzD8iuVPCNnreRVFjcklsVutcH_W1NY5wSjm5AGNDgnH2hcurTOSEeMEactBvrj_jpSN9HdMsJErQ8RBhq8T3QL03Zw8IkCnf2wjhggIjU7yHRAMMn5ica02PU1edHDEPHNsZ6T318-_7r5Vtx9_3p7c31XGCVYKiqoBWsBq04CN7wEaWVvrASoVG2MLK3seCsbZB0X0NbQ9V3XiDrfXzWqt_KcvDvMnYP_u2BMenTR4DDAhH6Jus4uNFLxJ0FeqUo1rMxgeQBN8DEG7PUc3Ahh1ZzpfSr6MRW9t1y3tX5MRYvcd3H8YOlGtKeuYwxZf3vUIRoY-gCTcfGEiUbs18zY1QHD7NqDw6CjcTgZtC5HlrT17olF_gNuU6rF</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16464805</pqid></control><display><type>article</type><title>5-hydroxytryptamine receptor subtype messenger RNAs in human dorsal root ganglia: a polymerase chain reaction study</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Pierce, P.A ; Xie, G.-X ; Meuser, T ; Peroutka, S.J</creator><creatorcontrib>Pierce, P.A ; Xie, G.-X ; Meuser, T ; Peroutka, S.J</creatorcontrib><description>Although serotonin has been shown to play an important role in peripheral pain mechanisms, the specific subtypes of serotonin receptors involved in pain and hyperalgesia remain poorly understood. To date, no previous study has attempted to determine the presence of any serotonin receptor subtype in human dorsal root ganglia. In this study, the presence of messenger RNA for eight human serotonin receptor subtypes in lumbar dorsal root ganglia was detected using the method of polymerase chain reaction. Dorsal root ganglia were excised
post mortem from four patients. Oligonucleotide primers were chosen based on unique regions of complimentary DNA sequence for eight cloned human serotonin receptor subtypes (i.e. 5-HT
1A, 5-HT
1D
α
, 5-HT
1D
β
, 5-HT
1E, 5-HT
1F, 5-HT
2A, 5-HT
2C and 5-HT
7). The presence of 5-HT
1D
α
, 5-HT
1D
β
, 5-HT
1E, 5-HT
1F, 5-HT
2A and 5-HT
7 receptor subtype messenger RNA was detected in dorsal root ganglia from three of the four subjects. 5-HT
1A receptor subtype messenger RNA was detected in one of the four subjects. No 5-HT
2C receptor subtype messenger RNA could be detected.
Findings from this study may direct further efforts to determine the role of serotonin receptors in the peripheral nervous system.</description><identifier>ISSN: 0306-4522</identifier><identifier>EISSN: 1873-7544</identifier><identifier>DOI: 10.1016/S0306-4522(97)00235-2</identifier><identifier>PMID: 9316030</identifier><identifier>CODEN: NRSCDN</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>5-HT ; 5-hydroxytryptamine ; Aged ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; dorsal root ganglia ; Female ; Fundamental and applied biological sciences. Psychology ; Ganglia, Spinal - metabolism ; Humans ; Lumbosacral Region ; Male ; Middle Aged ; pain ; peripheral ; Polymerase Chain Reaction ; Receptors, Serotonin - genetics ; RNA, Messenger - metabolism ; serotonin ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience, 1997-12, Vol.81 (3), p.813-819</ispartof><rights>1997 IBRO</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-6a7209ae6b3a1c15a3d3fcd3aa647cc35d3b1938e0b12a97abfbb827873684fd3</citedby><cites>FETCH-LOGICAL-c420t-6a7209ae6b3a1c15a3d3fcd3aa647cc35d3b1938e0b12a97abfbb827873684fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0306-4522(97)00235-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2823413$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9316030$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pierce, P.A</creatorcontrib><creatorcontrib>Xie, G.-X</creatorcontrib><creatorcontrib>Meuser, T</creatorcontrib><creatorcontrib>Peroutka, S.J</creatorcontrib><title>5-hydroxytryptamine receptor subtype messenger RNAs in human dorsal root ganglia: a polymerase chain reaction study</title><title>Neuroscience</title><addtitle>Neuroscience</addtitle><description>Although serotonin has been shown to play an important role in peripheral pain mechanisms, the specific subtypes of serotonin receptors involved in pain and hyperalgesia remain poorly understood. To date, no previous study has attempted to determine the presence of any serotonin receptor subtype in human dorsal root ganglia. In this study, the presence of messenger RNA for eight human serotonin receptor subtypes in lumbar dorsal root ganglia was detected using the method of polymerase chain reaction. Dorsal root ganglia were excised
post mortem from four patients. Oligonucleotide primers were chosen based on unique regions of complimentary DNA sequence for eight cloned human serotonin receptor subtypes (i.e. 5-HT
1A, 5-HT
1D
α
, 5-HT
1D
β
, 5-HT
1E, 5-HT
1F, 5-HT
2A, 5-HT
2C and 5-HT
7). The presence of 5-HT
1D
α
, 5-HT
1D
β
, 5-HT
1E, 5-HT
1F, 5-HT
2A and 5-HT
7 receptor subtype messenger RNA was detected in dorsal root ganglia from three of the four subjects. 5-HT
1A receptor subtype messenger RNA was detected in one of the four subjects. No 5-HT
2C receptor subtype messenger RNA could be detected.
Findings from this study may direct further efforts to determine the role of serotonin receptors in the peripheral nervous system.</description><subject>5-HT</subject><subject>5-hydroxytryptamine</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>dorsal root ganglia</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ganglia, Spinal - metabolism</subject><subject>Humans</subject><subject>Lumbosacral Region</subject><subject>Male</subject><subject>Middle Aged</subject><subject>pain</subject><subject>peripheral</subject><subject>Polymerase Chain Reaction</subject><subject>Receptors, Serotonin - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>serotonin</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0306-4522</issn><issn>1873-7544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUlvFDEQhS0ECkPgJ0TyASE4NPHWG5coilgiRUFiOVvVdvWMUXe7sd0R_e_xZEZzjS91eF-Vq94j5IKzj5zx6vInk6wqVCnE-7b-wJiQZSGekQ1valnUpVLPyeaEvCSvYvzD8iuVPCNnreRVFjcklsVutcH_W1NY5wSjm5AGNDgnH2hcurTOSEeMEactBvrj_jpSN9HdMsJErQ8RBhq8T3QL03Zw8IkCnf2wjhggIjU7yHRAMMn5ica02PU1edHDEPHNsZ6T318-_7r5Vtx9_3p7c31XGCVYKiqoBWsBq04CN7wEaWVvrASoVG2MLK3seCsbZB0X0NbQ9V3XiDrfXzWqt_KcvDvMnYP_u2BMenTR4DDAhH6Jus4uNFLxJ0FeqUo1rMxgeQBN8DEG7PUc3Ahh1ZzpfSr6MRW9t1y3tX5MRYvcd3H8YOlGtKeuYwxZf3vUIRoY-gCTcfGEiUbs18zY1QHD7NqDw6CjcTgZtC5HlrT17olF_gNuU6rF</recordid><startdate>19971201</startdate><enddate>19971201</enddate><creator>Pierce, P.A</creator><creator>Xie, G.-X</creator><creator>Meuser, T</creator><creator>Peroutka, S.J</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19971201</creationdate><title>5-hydroxytryptamine receptor subtype messenger RNAs in human dorsal root ganglia: a polymerase chain reaction study</title><author>Pierce, P.A ; Xie, G.-X ; Meuser, T ; Peroutka, S.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-6a7209ae6b3a1c15a3d3fcd3aa647cc35d3b1938e0b12a97abfbb827873684fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>5-HT</topic><topic>5-hydroxytryptamine</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>dorsal root ganglia</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ganglia, Spinal - metabolism</topic><topic>Humans</topic><topic>Lumbosacral Region</topic><topic>Male</topic><topic>Middle Aged</topic><topic>pain</topic><topic>peripheral</topic><topic>Polymerase Chain Reaction</topic><topic>Receptors, Serotonin - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>serotonin</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pierce, P.A</creatorcontrib><creatorcontrib>Xie, G.-X</creatorcontrib><creatorcontrib>Meuser, T</creatorcontrib><creatorcontrib>Peroutka, S.J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pierce, P.A</au><au>Xie, G.-X</au><au>Meuser, T</au><au>Peroutka, S.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>5-hydroxytryptamine receptor subtype messenger RNAs in human dorsal root ganglia: a polymerase chain reaction study</atitle><jtitle>Neuroscience</jtitle><addtitle>Neuroscience</addtitle><date>1997-12-01</date><risdate>1997</risdate><volume>81</volume><issue>3</issue><spage>813</spage><epage>819</epage><pages>813-819</pages><issn>0306-4522</issn><eissn>1873-7544</eissn><coden>NRSCDN</coden><abstract>Although serotonin has been shown to play an important role in peripheral pain mechanisms, the specific subtypes of serotonin receptors involved in pain and hyperalgesia remain poorly understood. To date, no previous study has attempted to determine the presence of any serotonin receptor subtype in human dorsal root ganglia. In this study, the presence of messenger RNA for eight human serotonin receptor subtypes in lumbar dorsal root ganglia was detected using the method of polymerase chain reaction. Dorsal root ganglia were excised
post mortem from four patients. Oligonucleotide primers were chosen based on unique regions of complimentary DNA sequence for eight cloned human serotonin receptor subtypes (i.e. 5-HT
1A, 5-HT
1D
α
, 5-HT
1D
β
, 5-HT
1E, 5-HT
1F, 5-HT
2A, 5-HT
2C and 5-HT
7). The presence of 5-HT
1D
α
, 5-HT
1D
β
, 5-HT
1E, 5-HT
1F, 5-HT
2A and 5-HT
7 receptor subtype messenger RNA was detected in dorsal root ganglia from three of the four subjects. 5-HT
1A receptor subtype messenger RNA was detected in one of the four subjects. No 5-HT
2C receptor subtype messenger RNA could be detected.
Findings from this study may direct further efforts to determine the role of serotonin receptors in the peripheral nervous system.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9316030</pmid><doi>10.1016/S0306-4522(97)00235-2</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0306-4522 |
| ispartof | Neuroscience, 1997-12, Vol.81 (3), p.813-819 |
| issn | 0306-4522 1873-7544 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79318341 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 5-HT 5-hydroxytryptamine Aged Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors dorsal root ganglia Female Fundamental and applied biological sciences. Psychology Ganglia, Spinal - metabolism Humans Lumbosacral Region Male Middle Aged pain peripheral Polymerase Chain Reaction Receptors, Serotonin - genetics RNA, Messenger - metabolism serotonin Vertebrates: nervous system and sense organs |
| title | 5-hydroxytryptamine receptor subtype messenger RNAs in human dorsal root ganglia: a polymerase chain reaction study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T17%3A54%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=5-hydroxytryptamine%20receptor%20subtype%20messenger%20RNAs%20in%20human%20dorsal%20root%20ganglia:%20a%20polymerase%20chain%20reaction%20study&rft.jtitle=Neuroscience&rft.au=Pierce,%20P.A&rft.date=1997-12-01&rft.volume=81&rft.issue=3&rft.spage=813&rft.epage=819&rft.pages=813-819&rft.issn=0306-4522&rft.eissn=1873-7544&rft.coden=NRSCDN&rft_id=info:doi/10.1016/S0306-4522(97)00235-2&rft_dat=%3Cproquest_cross%3E16464805%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16464805&rft_id=info:pmid/9316030&rft_els_id=S0306452297002352&rfr_iscdi=true |