Antigen presentation by B cells and macrophages of cytochrome c and its antigenic fragment when conjugated to the surface of liposomes
An in vitro antigen presentation system was used to study how antigens coupled to the surface of phospholipid vesicles (liposomes) are presented to antigen specific T cells. Liposome-bound pigeon cytochrome c (PCC) was 30–40-fold more potent than free PCC when peritoneal macrophages were the present...
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| Veröffentlicht in: | Vaccine 1989-10, Vol.7 (5), p.401-408 |
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| container_title | Vaccine |
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| creator | Dal Monte, Paul R. Szoka, Francis C. |
| description | An
in vitro antigen presentation system was used to study how antigens coupled to the surface of phospholipid vesicles (liposomes) are presented to antigen specific T cells. Liposome-bound pigeon cytochrome c (PCC) was 30–40-fold more potent than free PCC when peritoneal macrophages were the presenting cell. B cells presented surface-bound PCC, albeit less efficiently than unmodified PCC. Surface-bound peptide epitope was presented by both cell types, but not as efficiently as unmodified peptide. With the T cell epitope, antigen processing was not required since glutaraldehyde fixed cells could present surface-bound peptide. |
| doi_str_mv | 10.1016/0264-410X(89)90153-9 |
| format | Article |
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in vitro antigen presentation system was used to study how antigens coupled to the surface of phospholipid vesicles (liposomes) are presented to antigen specific T cells. Liposome-bound pigeon cytochrome c (PCC) was 30–40-fold more potent than free PCC when peritoneal macrophages were the presenting cell. B cells presented surface-bound PCC, albeit less efficiently than unmodified PCC. Surface-bound peptide epitope was presented by both cell types, but not as efficiently as unmodified peptide. With the T cell epitope, antigen processing was not required since glutaraldehyde fixed cells could present surface-bound peptide.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/0264-410X(89)90153-9</identifier><identifier>PMID: 2554605</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adjuvants, Immunologic ; Analysis of the immune response. Humoral and cellular immunity ; Animals ; antigen presentation ; Antigen-Presenting Cells - immunology ; B cell ; B-Lymphocytes - immunology ; Biological and medical sciences ; Cell interactions ; Cell Line ; Columbidae ; Cytochrome c ; Cytochrome c Group - immunology ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Hybridomas ; Immunobiology ; Interleukin-2 - biosynthesis ; liposomes ; Liposomes - immunology ; macrophage ; Macrophages - immunology ; Male ; Mice ; Mice, Inbred C3H ; T-Lymphocytes, Helper-Inducer - metabolism</subject><ispartof>Vaccine, 1989-10, Vol.7 (5), p.401-408</ispartof><rights>1989</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-fe7df6329a08edc2265b0b4c6101a8e4261336eba3971c3c15fe9a69420cce8d3</citedby><cites>FETCH-LOGICAL-c417t-fe7df6329a08edc2265b0b4c6101a8e4261336eba3971c3c15fe9a69420cce8d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0264410X89901539$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7348742$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2554605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dal Monte, Paul R.</creatorcontrib><creatorcontrib>Szoka, Francis C.</creatorcontrib><title>Antigen presentation by B cells and macrophages of cytochrome c and its antigenic fragment when conjugated to the surface of liposomes</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>An
in vitro antigen presentation system was used to study how antigens coupled to the surface of phospholipid vesicles (liposomes) are presented to antigen specific T cells. Liposome-bound pigeon cytochrome c (PCC) was 30–40-fold more potent than free PCC when peritoneal macrophages were the presenting cell. B cells presented surface-bound PCC, albeit less efficiently than unmodified PCC. Surface-bound peptide epitope was presented by both cell types, but not as efficiently as unmodified peptide. With the T cell epitope, antigen processing was not required since glutaraldehyde fixed cells could present surface-bound peptide.</description><subject>Adjuvants, Immunologic</subject><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>antigen presentation</subject><subject>Antigen-Presenting Cells - immunology</subject><subject>B cell</subject><subject>B-Lymphocytes - immunology</subject><subject>Biological and medical sciences</subject><subject>Cell interactions</subject><subject>Cell Line</subject><subject>Columbidae</subject><subject>Cytochrome c</subject><subject>Cytochrome c Group - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Hybridomas</subject><subject>Immunobiology</subject><subject>Interleukin-2 - biosynthesis</subject><subject>liposomes</subject><subject>Liposomes - immunology</subject><subject>macrophage</subject><subject>Macrophages - immunology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>T-Lymphocytes, Helper-Inducer - metabolism</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi0EKtPCG4DkBUJlEfAtF28qlYqbVIkNSOws5-RkxlUSB9sBzQvw3Dgzo1nCyovznc_2_xPygrO3nPHqHROVKhRnP64b_UYzXspCPyIb3tSyECVvHpPNGXlKLmN8YIyVkusLciHKUlWs3JA_t1NyW5zoHDDilGxyfqLtnr6ngMMQqZ06OloIft7ZLUbqewr75GEX_IgUDnOXVu7gcUD7YLdjVtHfu-wFPz0sW5uwo8nTtEMal9BbwNU0uNnH7InPyJPeDhGfn84r8v3jh293n4v7r5--3N3eF6B4nYoe666vpNCWNdiBEFXZslZBlQOxDSpRcSkrbK3UNQcJvOxR20orwQCw6eQVeX30zsH_XDAmM7q4ftRO6Jdoai1zjqL-L8hLxZhSIoPqCOaIYgzYmzm40Ya94cysPZm1BLOWYBptDj0ZnddenvxLO2J3XjoVk-evTnMbwQ450wlcPGO1VE19uP3miGEO7ZfDYCI4nAA7FxCS6bz79zv-AnaBsKE</recordid><startdate>19891001</startdate><enddate>19891001</enddate><creator>Dal Monte, Paul R.</creator><creator>Szoka, Francis C.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19891001</creationdate><title>Antigen presentation by B cells and macrophages of cytochrome c and its antigenic fragment when conjugated to the surface of liposomes</title><author>Dal Monte, Paul R. ; Szoka, Francis C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-fe7df6329a08edc2265b0b4c6101a8e4261336eba3971c3c15fe9a69420cce8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adjuvants, Immunologic</topic><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>antigen presentation</topic><topic>Antigen-Presenting Cells - immunology</topic><topic>B cell</topic><topic>B-Lymphocytes - immunology</topic><topic>Biological and medical sciences</topic><topic>Cell interactions</topic><topic>Cell Line</topic><topic>Columbidae</topic><topic>Cytochrome c</topic><topic>Cytochrome c Group - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Hybridomas</topic><topic>Immunobiology</topic><topic>Interleukin-2 - biosynthesis</topic><topic>liposomes</topic><topic>Liposomes - immunology</topic><topic>macrophage</topic><topic>Macrophages - immunology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>T-Lymphocytes, Helper-Inducer - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dal Monte, Paul R.</creatorcontrib><creatorcontrib>Szoka, Francis C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dal Monte, Paul R.</au><au>Szoka, Francis C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antigen presentation by B cells and macrophages of cytochrome c and its antigenic fragment when conjugated to the surface of liposomes</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>1989-10-01</date><risdate>1989</risdate><volume>7</volume><issue>5</issue><spage>401</spage><epage>408</epage><pages>401-408</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>An
in vitro antigen presentation system was used to study how antigens coupled to the surface of phospholipid vesicles (liposomes) are presented to antigen specific T cells. Liposome-bound pigeon cytochrome c (PCC) was 30–40-fold more potent than free PCC when peritoneal macrophages were the presenting cell. B cells presented surface-bound PCC, albeit less efficiently than unmodified PCC. Surface-bound peptide epitope was presented by both cell types, but not as efficiently as unmodified peptide. With the T cell epitope, antigen processing was not required since glutaraldehyde fixed cells could present surface-bound peptide.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>2554605</pmid><doi>10.1016/0264-410X(89)90153-9</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 1989-10, Vol.7 (5), p.401-408 |
| issn | 0264-410X 1873-2518 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79315327 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adjuvants, Immunologic Analysis of the immune response. Humoral and cellular immunity Animals antigen presentation Antigen-Presenting Cells - immunology B cell B-Lymphocytes - immunology Biological and medical sciences Cell interactions Cell Line Columbidae Cytochrome c Cytochrome c Group - immunology Fundamental and applied biological sciences. Psychology Fundamental immunology Hybridomas Immunobiology Interleukin-2 - biosynthesis liposomes Liposomes - immunology macrophage Macrophages - immunology Male Mice Mice, Inbred C3H T-Lymphocytes, Helper-Inducer - metabolism |
| title | Antigen presentation by B cells and macrophages of cytochrome c and its antigenic fragment when conjugated to the surface of liposomes |
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