ASTROCYTE-CONDITIONED MEDIUM INDUCES BLOOD-BRAIN BARRIER PROPERTIES IN ENDOTHELIAL CELLS
SUMMARY 1. We evaluated the induction of endothelial barrier functions by the type 1 astrocyte‐derived supernatant in culture using horseradish peroxidase (HRP) as a parameter. 2. A gel filtration fraction (molecular weight 16‐20 kDa) of type 1 astrocyte‐derived supernatant reduced plasmalemmal vesi...
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| Veröffentlicht in: | Clinical and experimental pharmacology & physiology 1997-10, Vol.24 (9-10), p.710-713 |
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| container_issue | 9-10 |
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| container_title | Clinical and experimental pharmacology & physiology |
| container_volume | 24 |
| creator | Yamagata, Kazuo Tagami, Motoki Nara, Yasuo Mitani, Mitsuaki Kubota, Akiyoshi Fujino, Hideaki Numano, Fujio Kato§, Taiji Yamori#, Yukio |
| description | SUMMARY
1. We evaluated the induction of endothelial barrier functions by the type 1 astrocyte‐derived supernatant in culture using horseradish peroxidase (HRP) as a parameter.
2. A gel filtration fraction (molecular weight 16‐20 kDa) of type 1 astrocyte‐derived supernatant reduced plasmalemmal vesicles and induced tight junctions in endothelial cells.
3. In addition, this fraction promoted the uptake of [3H]‐glucosamine by endothelial cells. This cell activity disappeared after heating at 95°C for 10 min and was sensitive to trypsin.
4. These results suggest that a type 1 astrocyte‐derived protein factor(s) promotes proteoglycan synthesis with a resultant increase in charge selectivity in endothelial cells and plays an important role in the induction of blood‐brain barrier functions. |
| doi_str_mv | 10.1111/j.1440-1681.1997.tb02117.x |
| format | Article |
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1. We evaluated the induction of endothelial barrier functions by the type 1 astrocyte‐derived supernatant in culture using horseradish peroxidase (HRP) as a parameter.
2. A gel filtration fraction (molecular weight 16‐20 kDa) of type 1 astrocyte‐derived supernatant reduced plasmalemmal vesicles and induced tight junctions in endothelial cells.
3. In addition, this fraction promoted the uptake of [3H]‐glucosamine by endothelial cells. This cell activity disappeared after heating at 95°C for 10 min and was sensitive to trypsin.
4. These results suggest that a type 1 astrocyte‐derived protein factor(s) promotes proteoglycan synthesis with a resultant increase in charge selectivity in endothelial cells and plays an important role in the induction of blood‐brain barrier functions.</description><identifier>ISSN: 0305-1870</identifier><identifier>EISSN: 1440-1681</identifier><identifier>DOI: 10.1111/j.1440-1681.1997.tb02117.x</identifier><identifier>PMID: 9315374</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; astrocyte ; Astrocytes - drug effects ; Astrocytes - metabolism ; Astrocytes - physiology ; blood-brain barrier ; Blood-Brain Barrier - drug effects ; Blood-Brain Barrier - physiology ; Cells, Cultured ; Chromatography, Gel ; Culture Media, Conditioned - pharmacology ; endothelial cells ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - physiology ; extracellular matrix ; Glucosamine - metabolism ; Horseradish Peroxidase ; Microscopy, Electron ; Molecular Weight ; proteoglycan ; Rats ; tight junction ; ultrastructure</subject><ispartof>Clinical and experimental pharmacology & physiology, 1997-10, Vol.24 (9-10), p.710-713</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4070-274e0baf9cc32f5732e1ba1238a8a2db857894d1dfe7f43e38196b74cc0aa87d3</citedby><cites>FETCH-LOGICAL-c4070-274e0baf9cc32f5732e1ba1238a8a2db857894d1dfe7f43e38196b74cc0aa87d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1440-1681.1997.tb02117.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1440-1681.1997.tb02117.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9315374$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamagata, Kazuo</creatorcontrib><creatorcontrib>Tagami, Motoki</creatorcontrib><creatorcontrib>Nara, Yasuo</creatorcontrib><creatorcontrib>Mitani, Mitsuaki</creatorcontrib><creatorcontrib>Kubota, Akiyoshi</creatorcontrib><creatorcontrib>Fujino, Hideaki</creatorcontrib><creatorcontrib>Numano, Fujio</creatorcontrib><creatorcontrib>Kato§, Taiji</creatorcontrib><creatorcontrib>Yamori#, Yukio</creatorcontrib><title>ASTROCYTE-CONDITIONED MEDIUM INDUCES BLOOD-BRAIN BARRIER PROPERTIES IN ENDOTHELIAL CELLS</title><title>Clinical and experimental pharmacology & physiology</title><addtitle>Clin Exp Pharmacol Physiol</addtitle><description>SUMMARY
1. We evaluated the induction of endothelial barrier functions by the type 1 astrocyte‐derived supernatant in culture using horseradish peroxidase (HRP) as a parameter.
2. A gel filtration fraction (molecular weight 16‐20 kDa) of type 1 astrocyte‐derived supernatant reduced plasmalemmal vesicles and induced tight junctions in endothelial cells.
3. In addition, this fraction promoted the uptake of [3H]‐glucosamine by endothelial cells. This cell activity disappeared after heating at 95°C for 10 min and was sensitive to trypsin.
4. These results suggest that a type 1 astrocyte‐derived protein factor(s) promotes proteoglycan synthesis with a resultant increase in charge selectivity in endothelial cells and plays an important role in the induction of blood‐brain barrier functions.</description><subject>Animals</subject><subject>astrocyte</subject><subject>Astrocytes - drug effects</subject><subject>Astrocytes - metabolism</subject><subject>Astrocytes - physiology</subject><subject>blood-brain barrier</subject><subject>Blood-Brain Barrier - drug effects</subject><subject>Blood-Brain Barrier - physiology</subject><subject>Cells, Cultured</subject><subject>Chromatography, Gel</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>endothelial cells</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - physiology</subject><subject>extracellular matrix</subject><subject>Glucosamine - metabolism</subject><subject>Horseradish Peroxidase</subject><subject>Microscopy, Electron</subject><subject>Molecular Weight</subject><subject>proteoglycan</subject><subject>Rats</subject><subject>tight junction</subject><subject>ultrastructure</subject><issn>0305-1870</issn><issn>1440-1681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkFFvmzAQx61pU5d1_QiVUB_2BrWx4UwfKhFwG0sUUkK07skyYKSkSdPhRE2__YgS5X33Yun-d7-TfwjdEOyRoW6XHmEMuyTkxCNRBN62xj4h4O2_oNE5-opGmOLAJRzwd_TD2iXGOMAhvUAXESUBBTZCL_GsKovkTyXcpMhTWckiF6nzJFI5f3Jkns4TMXPGWVGk7riMZe6M47KUonSmZTEVZSWHeOiKPC2qichknDmJyLLZT_St0ytrrk7vJZo_iCqZuFnxKJM4cxuGAbs-MINr3UVNQ_0uAOobUmviU6659tuaB8Aj1pK2M9AxaignUVgDaxqsNYeWXqJfR-57v_m7M3ar1gvbmNVKv5nNzioYvsoAwmHw7jjY9Btre9Op936x1v2nIlgdtKqlOrhTB3fqoFWdtKr9sHx9urKr16Y9r548Dvn9Mf9YrMznf5BVIqZA8ABwj4CF3Zr9GaD7VxUChUD9zh8VnhI-icYP6pn-A59Tjqg</recordid><startdate>199710</startdate><enddate>199710</enddate><creator>Yamagata, Kazuo</creator><creator>Tagami, Motoki</creator><creator>Nara, Yasuo</creator><creator>Mitani, Mitsuaki</creator><creator>Kubota, Akiyoshi</creator><creator>Fujino, Hideaki</creator><creator>Numano, Fujio</creator><creator>Kato§, Taiji</creator><creator>Yamori#, Yukio</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199710</creationdate><title>ASTROCYTE-CONDITIONED MEDIUM INDUCES BLOOD-BRAIN BARRIER PROPERTIES IN ENDOTHELIAL CELLS</title><author>Yamagata, Kazuo ; Tagami, Motoki ; Nara, Yasuo ; Mitani, Mitsuaki ; Kubota, Akiyoshi ; Fujino, Hideaki ; Numano, Fujio ; Kato§, Taiji ; Yamori#, Yukio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4070-274e0baf9cc32f5732e1ba1238a8a2db857894d1dfe7f43e38196b74cc0aa87d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>astrocyte</topic><topic>Astrocytes - drug effects</topic><topic>Astrocytes - metabolism</topic><topic>Astrocytes - physiology</topic><topic>blood-brain barrier</topic><topic>Blood-Brain Barrier - drug effects</topic><topic>Blood-Brain Barrier - physiology</topic><topic>Cells, Cultured</topic><topic>Chromatography, Gel</topic><topic>Culture Media, Conditioned - pharmacology</topic><topic>endothelial cells</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - physiology</topic><topic>extracellular matrix</topic><topic>Glucosamine - metabolism</topic><topic>Horseradish Peroxidase</topic><topic>Microscopy, Electron</topic><topic>Molecular Weight</topic><topic>proteoglycan</topic><topic>Rats</topic><topic>tight junction</topic><topic>ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamagata, Kazuo</creatorcontrib><creatorcontrib>Tagami, Motoki</creatorcontrib><creatorcontrib>Nara, Yasuo</creatorcontrib><creatorcontrib>Mitani, Mitsuaki</creatorcontrib><creatorcontrib>Kubota, Akiyoshi</creatorcontrib><creatorcontrib>Fujino, Hideaki</creatorcontrib><creatorcontrib>Numano, Fujio</creatorcontrib><creatorcontrib>Kato§, Taiji</creatorcontrib><creatorcontrib>Yamori#, Yukio</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental pharmacology & physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamagata, Kazuo</au><au>Tagami, Motoki</au><au>Nara, Yasuo</au><au>Mitani, Mitsuaki</au><au>Kubota, Akiyoshi</au><au>Fujino, Hideaki</au><au>Numano, Fujio</au><au>Kato§, Taiji</au><au>Yamori#, Yukio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ASTROCYTE-CONDITIONED MEDIUM INDUCES BLOOD-BRAIN BARRIER PROPERTIES IN ENDOTHELIAL CELLS</atitle><jtitle>Clinical and experimental pharmacology & physiology</jtitle><addtitle>Clin Exp Pharmacol Physiol</addtitle><date>1997-10</date><risdate>1997</risdate><volume>24</volume><issue>9-10</issue><spage>710</spage><epage>713</epage><pages>710-713</pages><issn>0305-1870</issn><eissn>1440-1681</eissn><abstract>SUMMARY
1. We evaluated the induction of endothelial barrier functions by the type 1 astrocyte‐derived supernatant in culture using horseradish peroxidase (HRP) as a parameter.
2. A gel filtration fraction (molecular weight 16‐20 kDa) of type 1 astrocyte‐derived supernatant reduced plasmalemmal vesicles and induced tight junctions in endothelial cells.
3. In addition, this fraction promoted the uptake of [3H]‐glucosamine by endothelial cells. This cell activity disappeared after heating at 95°C for 10 min and was sensitive to trypsin.
4. These results suggest that a type 1 astrocyte‐derived protein factor(s) promotes proteoglycan synthesis with a resultant increase in charge selectivity in endothelial cells and plays an important role in the induction of blood‐brain barrier functions.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9315374</pmid><doi>10.1111/j.1440-1681.1997.tb02117.x</doi><tpages>4</tpages></addata></record> |
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| ispartof | Clinical and experimental pharmacology & physiology, 1997-10, Vol.24 (9-10), p.710-713 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals astrocyte Astrocytes - drug effects Astrocytes - metabolism Astrocytes - physiology blood-brain barrier Blood-Brain Barrier - drug effects Blood-Brain Barrier - physiology Cells, Cultured Chromatography, Gel Culture Media, Conditioned - pharmacology endothelial cells Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Endothelium, Vascular - physiology extracellular matrix Glucosamine - metabolism Horseradish Peroxidase Microscopy, Electron Molecular Weight proteoglycan Rats tight junction ultrastructure |
| title | ASTROCYTE-CONDITIONED MEDIUM INDUCES BLOOD-BRAIN BARRIER PROPERTIES IN ENDOTHELIAL CELLS |
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