Characterization of a highly conserved gene (OS4) amplified with CDK4 in human sarcomas
Amplification and overexpression of genes involved in cellular growth control occur frequently in human cancers. Here, we report characterization of the full length OS4 cDNA derived from 12q13-q15 (Su et al., Proc. Natl. Acad. Sci. USA, 91: 9121-9125, 1994), a region frequently amplified in sarcomas...
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| Veröffentlicht in: | Oncogene 1997-09, Vol.15 (11), p.1289-1294 |
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| creator | SU, Y. A LEE, M. M HUTTER, C. M MELTZER, P. S |
| description | Amplification and overexpression of genes involved in cellular growth control occur frequently in human cancers. Here, we report characterization of the full length OS4 cDNA derived from 12q13-q15 (Su et al., Proc. Natl. Acad. Sci. USA, 91: 9121-9125, 1994), a region frequently amplified in sarcomas and brain tumors. This cDNA consists of 4833 base pairs (bp) encoding an open reading frame (ORF) of 283 amino acids. The ORF predicts a water-soluble acidic (pI 5.50) polypeptide with a molecular weight of 31759. Database searches revealed highly significant similarity between OS4 and eight proteins predicted from genomic sequences of Caenorhabditis elegans, Schizosaccaharomyces pombe, and Saccharomyces cerevisiae. Thus, OS4 defines a novel evolutionarily conserved gene superfamily. Northern and database analyses revealed OS4 transcripts in numerous human tissues demonstrating its ubiquitous expression. We also observed overexpression of OS4 in three cancer cell lines with amplification of this gene. Furthermore, we detected OS4 amplification in 5/5 primary sarcomas with known amplification of the closely linked marker CDK4. These results demonstrate that the highly conserved OS4 gene is frequently included in the 12q13-q15 amplicon and may contribute to the development of a subset of sarcomas. |
| doi_str_mv | 10.1038/sj.onc.1201294 |
| format | Article |
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Northern and database analyses revealed OS4 transcripts in numerous human tissues demonstrating its ubiquitous expression. We also observed overexpression of OS4 in three cancer cell lines with amplification of this gene. Furthermore, we detected OS4 amplification in 5/5 primary sarcomas with known amplification of the closely linked marker CDK4. These results demonstrate that the highly conserved OS4 gene is frequently included in the 12q13-q15 amplicon and may contribute to the development of a subset of sarcomas.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/sj.onc.1201294</identifier><identifier>PMID: 9315096</identifier><language>eng</language><publisher>Basingstoke: Nature Publishing</publisher><subject>Amino Acid Sequence ; Biological and medical sciences ; Blotting, Southern ; Brain tumors ; Caenorhabditis elegans ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Conserved Sequence ; Cyclin-Dependent Kinase 4 ; Cyclin-Dependent Kinases - genetics ; Evolution, Molecular ; Fundamental and applied biological sciences. Psychology ; Gene Amplification ; Gene Expression Regulation, Neoplastic ; Humans ; Molecular and cellular biology ; Molecular Sequence Data ; Molecular weight ; Nuclear Proteins ; Open reading frames ; Phosphoprotein Phosphatases ; Proteins - genetics ; Proteins - metabolism ; Proto-Oncogene Proteins ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Sarcoma ; Sarcoma - genetics ; Schizosaccharomyces pombe ; Sequence Analysis, DNA ; Tissue Distribution ; Tumor cell lines ; Tumor Cells, Cultured</subject><ispartof>Oncogene, 1997-09, Vol.15 (11), p.1289-1294</ispartof><rights>1997 INIST-CNRS</rights><rights>Macmillan Publishers Limited 1997.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-89cb3f89cde265e150d9fa367883f3c48dc877f41bc8226c9cedc37afa65eb1f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2822085$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9315096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SU, Y. A</creatorcontrib><creatorcontrib>LEE, M. M</creatorcontrib><creatorcontrib>HUTTER, C. M</creatorcontrib><creatorcontrib>MELTZER, P. S</creatorcontrib><title>Characterization of a highly conserved gene (OS4) amplified with CDK4 in human sarcomas</title><title>Oncogene</title><addtitle>Oncogene</addtitle><description>Amplification and overexpression of genes involved in cellular growth control occur frequently in human cancers. Here, we report characterization of the full length OS4 cDNA derived from 12q13-q15 (Su et al., Proc. Natl. Acad. Sci. USA, 91: 9121-9125, 1994), a region frequently amplified in sarcomas and brain tumors. This cDNA consists of 4833 base pairs (bp) encoding an open reading frame (ORF) of 283 amino acids. The ORF predicts a water-soluble acidic (pI 5.50) polypeptide with a molecular weight of 31759. Database searches revealed highly significant similarity between OS4 and eight proteins predicted from genomic sequences of Caenorhabditis elegans, Schizosaccaharomyces pombe, and Saccharomyces cerevisiae. Thus, OS4 defines a novel evolutionarily conserved gene superfamily. Northern and database analyses revealed OS4 transcripts in numerous human tissues demonstrating its ubiquitous expression. We also observed overexpression of OS4 in three cancer cell lines with amplification of this gene. Furthermore, we detected OS4 amplification in 5/5 primary sarcomas with known amplification of the closely linked marker CDK4. These results demonstrate that the highly conserved OS4 gene is frequently included in the 12q13-q15 amplicon and may contribute to the development of a subset of sarcomas.</description><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Brain tumors</subject><subject>Caenorhabditis elegans</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Conserved Sequence</subject><subject>Cyclin-Dependent Kinase 4</subject><subject>Cyclin-Dependent Kinases - genetics</subject><subject>Evolution, Molecular</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Amplification</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Molecular weight</subject><subject>Nuclear Proteins</subject><subject>Open reading frames</subject><subject>Phosphoprotein Phosphatases</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Proto-Oncogene Proteins</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Sarcoma</subject><subject>Sarcoma - genetics</subject><subject>Schizosaccharomyces pombe</subject><subject>Sequence Analysis, DNA</subject><subject>Tissue Distribution</subject><subject>Tumor cell lines</subject><subject>Tumor Cells, Cultured</subject><issn>0950-9232</issn><issn>1476-5594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1r3DAQxUVpSbdpr7kFBC2lOXirL9vSsWySpjSQQxtyFLOyFGuxpY1kJ6R_fVVicsillxmY95thHg-hI0rWlHD5Ne_WMZg1ZYQyJV6hFRVtU9W1Eq_RiqiaVIpx9ha9y3lHCGkVYQfoQHFaE9Ws0M2mhwRmssn_gcnHgKPDgHt_2w-P2MSQbbq3Hb61weIvV7_ECYZxP3jny_DBTz3enP4U2AfczyMEnCGZOEJ-j944GLL9sPRDdH1-9ntzUV1eff-x-XZZGUHlVEllttyV2lnW1LY81SkHvGml5I4bITsj29YJujWSscYoYzvDW3BQ6C11_BB9frq7T_FutnnSo8_GDgMEG-es2-JUCML_C9KGSM5VW8CPL8BdnFMoJjRrBGWcKEYKtX6iTIo5J-v0PvkR0qOmRP8LRuedLsHoJZiycLycnbej7Z7xJYmif1p0yAYGlyAYn58xVuwTWfO_7qmVNw</recordid><startdate>199709</startdate><enddate>199709</enddate><creator>SU, Y. 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Psychology</topic><topic>Gene Amplification</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Molecular weight</topic><topic>Nuclear Proteins</topic><topic>Open reading frames</topic><topic>Phosphoprotein Phosphatases</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>Proto-Oncogene Proteins</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Sarcoma</topic><topic>Sarcoma - genetics</topic><topic>Schizosaccharomyces pombe</topic><topic>Sequence Analysis, DNA</topic><topic>Tissue Distribution</topic><topic>Tumor cell lines</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SU, Y. A</creatorcontrib><creatorcontrib>LEE, M. M</creatorcontrib><creatorcontrib>HUTTER, C. 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A</au><au>LEE, M. M</au><au>HUTTER, C. M</au><au>MELTZER, P. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a highly conserved gene (OS4) amplified with CDK4 in human sarcomas</atitle><jtitle>Oncogene</jtitle><addtitle>Oncogene</addtitle><date>1997-09</date><risdate>1997</risdate><volume>15</volume><issue>11</issue><spage>1289</spage><epage>1294</epage><pages>1289-1294</pages><issn>0950-9232</issn><eissn>1476-5594</eissn><abstract>Amplification and overexpression of genes involved in cellular growth control occur frequently in human cancers. Here, we report characterization of the full length OS4 cDNA derived from 12q13-q15 (Su et al., Proc. Natl. Acad. Sci. USA, 91: 9121-9125, 1994), a region frequently amplified in sarcomas and brain tumors. This cDNA consists of 4833 base pairs (bp) encoding an open reading frame (ORF) of 283 amino acids. 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| source | MEDLINE; SpringerLink Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Nature Journals Online |
| subjects | Amino Acid Sequence Biological and medical sciences Blotting, Southern Brain tumors Caenorhabditis elegans Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Conserved Sequence Cyclin-Dependent Kinase 4 Cyclin-Dependent Kinases - genetics Evolution, Molecular Fundamental and applied biological sciences. Psychology Gene Amplification Gene Expression Regulation, Neoplastic Humans Molecular and cellular biology Molecular Sequence Data Molecular weight Nuclear Proteins Open reading frames Phosphoprotein Phosphatases Proteins - genetics Proteins - metabolism Proto-Oncogene Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Sarcoma Sarcoma - genetics Schizosaccharomyces pombe Sequence Analysis, DNA Tissue Distribution Tumor cell lines Tumor Cells, Cultured |
| title | Characterization of a highly conserved gene (OS4) amplified with CDK4 in human sarcomas |
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