Detection and cloning of epidermal zinc-alpha 2-glycoprotein cDNA and expression in normal human skin and in tumors
Zinc-alpha 2-glycoprotein (Zn alpha 2gp) is almost ubiquitous in body fluids, and its antibody labels the corresponding secretory epithelia. We have found that Zn alpha 2gp is also expressed in human epidermis. We cloned the Zn alpha 2gp cDNA by screening our cDNA library, derived from epidermal ker...
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| Veröffentlicht in: | Journal of cellular biochemistry 1997-11, Vol.67 (2), p.216-222 |
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| container_title | Journal of cellular biochemistry |
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| creator | Lei, G Arany, I Selvanayagam, P Rajaraman, S Ram, S Brysk, H Tyring, S K Brysk, M M |
| description | Zinc-alpha 2-glycoprotein (Zn alpha 2gp) is almost ubiquitous in body fluids, and its antibody labels the corresponding secretory epithelia. We have found that Zn alpha 2gp is also expressed in human epidermis. We cloned the Zn alpha 2gp cDNA by screening our cDNA library, derived from epidermal keratinocytes, with a probe for prostate Zn alpha 2gp. It had complete nucleic acid sequence homology with that from prostate, including the signal peptide. Just as Zn alpha 2gp expression is higher in more differentiated breast tumors, so in skin tumors the highest mRNA levels occurred in the normal controls, the lowest in basal cell carcinomas (the least differentiated epidermal tumor type), and intermediate levels in squamous cell carcinomas and Merkel cell carcinomas. A similar increase in Zn alpha 2gp gene expression with differentiation was observed when epidermal keratinocytes were cultured in media that varied in cellular maturation potential. |
| doi_str_mv | 10.1002/(SICI)1097-4644(19971101)67:2<216::AID-JCB6>3.0.CO;2-# |
| format | Article |
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A similar increase in Zn alpha 2gp gene expression with differentiation was observed when epidermal keratinocytes were cultured in media that varied in cellular maturation potential.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - analysis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Gene Expression</subject><subject>Glycoproteins - genetics</subject><subject>Humans</subject><subject>Keratinocytes - metabolism</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>RNA, Messenger - analysis</subject><subject>Seminal Plasma Proteins</subject><subject>Skin Neoplasms - metabolism</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kd1u1DAQhS0EKkvhEZAiIaH2wovHzjrxgipts_wsqtgLyvXI2E4bSOwQJ1LL0-N0l954JGvOmZnzEXIBbAmM8Xdn33fV7hyYKmgu8_wMlCoAGJzLYs0_cJDr9Wa3pV-rS3khlmxZ7d9z-uYJWTxKnpIFKwSjXAB_Tl7E-IsxppTgJ-QkvWXJ5YLErRudGZvgM-1tZtrgG3-ThTpzfWPd0Ok2-9t4Q3Xb3-qM05v23oR-CKNrfGa23zYPOnfXDy7G2SZ9-_Cgu5067bP4uzl4pzJOXRjiS_Ks1m10r471lPz49PG6-kKv9p931eaKGhBMUuu4rUVu65KXuVk5MFIzuUqnSW0k50rmzDibm0JpyfXK5EUtNJQAVlgLWpyStwfftO6fycURuyYa17bauzBFLJQAEIqlxutDoxlCjIOrsR-aTg_3CAxnGogzDZyjxTla_E8DZYEcEw3ERANnGiiQYbVHnmxfH-dPPztnH02P4Yt_yxCLoA</recordid><startdate>19971101</startdate><enddate>19971101</enddate><creator>Lei, G</creator><creator>Arany, I</creator><creator>Selvanayagam, P</creator><creator>Rajaraman, S</creator><creator>Ram, S</creator><creator>Brysk, H</creator><creator>Tyring, S K</creator><creator>Brysk, M M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19971101</creationdate><title>Detection and cloning of epidermal zinc-alpha 2-glycoprotein cDNA and expression in normal human skin and in tumors</title><author>Lei, G ; Arany, I ; Selvanayagam, P ; Rajaraman, S ; Ram, S ; Brysk, H ; Tyring, S K ; Brysk, M M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1306-de2df34df8284c5e1c6a0654646ac6229640ced4c79a62a5c47f3a1811d3dd1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - analysis</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Gene Expression</topic><topic>Glycoproteins - genetics</topic><topic>Humans</topic><topic>Keratinocytes - metabolism</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>RNA, Messenger - analysis</topic><topic>Seminal Plasma Proteins</topic><topic>Skin Neoplasms - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lei, G</creatorcontrib><creatorcontrib>Arany, I</creatorcontrib><creatorcontrib>Selvanayagam, P</creatorcontrib><creatorcontrib>Rajaraman, S</creatorcontrib><creatorcontrib>Ram, S</creatorcontrib><creatorcontrib>Brysk, H</creatorcontrib><creatorcontrib>Tyring, S K</creatorcontrib><creatorcontrib>Brysk, M M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lei, G</au><au>Arany, I</au><au>Selvanayagam, P</au><au>Rajaraman, S</au><au>Ram, S</au><au>Brysk, H</au><au>Tyring, S K</au><au>Brysk, M M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and cloning of epidermal zinc-alpha 2-glycoprotein cDNA and expression in normal human skin and in tumors</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J Cell Biochem</addtitle><date>1997-11-01</date><risdate>1997</risdate><volume>67</volume><issue>2</issue><spage>216</spage><epage>222</epage><pages>216-222</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>Zinc-alpha 2-glycoprotein (Zn alpha 2gp) is almost ubiquitous in body fluids, and its antibody labels the corresponding secretory epithelia. 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| ispartof | Journal of cellular biochemistry, 1997-11, Vol.67 (2), p.216-222 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amino Acid Sequence Base Sequence Blotting, Northern Cells, Cultured Cloning, Molecular DNA, Complementary - analysis Electrophoresis, Polyacrylamide Gel Gene Expression Glycoproteins - genetics Humans Keratinocytes - metabolism Male Molecular Sequence Data RNA, Messenger - analysis Seminal Plasma Proteins Skin Neoplasms - metabolism |
| title | Detection and cloning of epidermal zinc-alpha 2-glycoprotein cDNA and expression in normal human skin and in tumors |
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