Changes in the polymorphonuclear leukocyte function of blood samples induced by storage time, temperature and agitation
We have investigated changes in polymorphonuclear leukocyte (PMN) functions of blood samples caused by such typical elements of laboratory handling as storage time, temperature and agitation. The blood of five healthy subjects was stored upright in test tubes at 4, 22 and 37°C over periods of 20 min...
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| Veröffentlicht in: | Journal of immunological methods 1997-08, Vol.206 (1), p.61-71 |
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| creator | Egger, Gerd Kukovetz, Elisabeth M Hayn, Marianne Fabjan, Judith S |
| description | We have investigated changes in polymorphonuclear leukocyte (PMN) functions of blood samples caused by such typical elements of laboratory handling as storage time, temperature and agitation. The blood of five healthy subjects was stored upright in test tubes at 4, 22 and 37°C over periods of 20 min, one, two, six and 24 h. Controlled agitation was performed on a shaker. The following PMN functional parameters were measured: the white blood cell count (WBC), migration, elastase (EL) release, reactive oxygen species (ROS) production and lipid peroxidation. Migration was determined in a whole-blood membrane filter assay; ROS production by latex-stimulated, luminol-enhanced chemiluminescence (CL) in a whole-blood assay; EL as EL
α1-antitrypsin complex; and lipid peroxidation by malondialdehyde (MDA) generation. The reactions after handling were compared with the values measured immediately after blood withdrawal which served as reference values of `genuine' PMN reactivity. The outstanding result was the marked scatter between the individual reactions. Overall, the proportion of migrating PMNs in the blood total decreased, while CL, correlating positively with MDA, increased with the time of storage. EL increased considerably in some of the samples. Agitation raised CL and MDA. The effect of temperature was apparent only after 24 h at 37°C. There was evidence that inhomogeneities in the blood samples were another interfering factor, since resuspension of sedimented blood after storage can be incomplete. In order to obtain reliable results from PMN functional tests, whole-blood assays and processing of blood samples within 20 min after blood withdrawal are recommended. |
| doi_str_mv | 10.1016/S0022-1759(97)00085-9 |
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α1-antitrypsin complex; and lipid peroxidation by malondialdehyde (MDA) generation. The reactions after handling were compared with the values measured immediately after blood withdrawal which served as reference values of `genuine' PMN reactivity. The outstanding result was the marked scatter between the individual reactions. Overall, the proportion of migrating PMNs in the blood total decreased, while CL, correlating positively with MDA, increased with the time of storage. EL increased considerably in some of the samples. Agitation raised CL and MDA. The effect of temperature was apparent only after 24 h at 37°C. There was evidence that inhomogeneities in the blood samples were another interfering factor, since resuspension of sedimented blood after storage can be incomplete. In order to obtain reliable results from PMN functional tests, whole-blood assays and processing of blood samples within 20 min after blood withdrawal are recommended.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/S0022-1759(97)00085-9</identifier><identifier>PMID: 9328569</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; Blood Preservation ; Blood samples ; Cell Movement ; Cold Temperature ; Enzyme Activation ; Female ; Handling ; Humans ; Leukocyte Elastase - blood ; Luminescent Measurements ; Male ; Malondialdehyde - blood ; Middle Aged ; Neutrophils - enzymology ; Neutrophils - physiology ; Polymorphonuclear leukocyte functions ; Storage ; Stress, Mechanical ; Temperature ; Thermal stress ; Time Factors</subject><ispartof>Journal of immunological methods, 1997-08, Vol.206 (1), p.61-71</ispartof><rights>1997 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-82cacaccd84c5e9650bbbbf237c5544d593217ca823bcc39d6767e768f8aa1b73</citedby><cites>FETCH-LOGICAL-c391t-82cacaccd84c5e9650bbbbf237c5544d593217ca823bcc39d6767e768f8aa1b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0022-1759(97)00085-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9328569$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Egger, Gerd</creatorcontrib><creatorcontrib>Kukovetz, Elisabeth M</creatorcontrib><creatorcontrib>Hayn, Marianne</creatorcontrib><creatorcontrib>Fabjan, Judith S</creatorcontrib><title>Changes in the polymorphonuclear leukocyte function of blood samples induced by storage time, temperature and agitation</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>We have investigated changes in polymorphonuclear leukocyte (PMN) functions of blood samples caused by such typical elements of laboratory handling as storage time, temperature and agitation. The blood of five healthy subjects was stored upright in test tubes at 4, 22 and 37°C over periods of 20 min, one, two, six and 24 h. Controlled agitation was performed on a shaker. The following PMN functional parameters were measured: the white blood cell count (WBC), migration, elastase (EL) release, reactive oxygen species (ROS) production and lipid peroxidation. Migration was determined in a whole-blood membrane filter assay; ROS production by latex-stimulated, luminol-enhanced chemiluminescence (CL) in a whole-blood assay; EL as EL
α1-antitrypsin complex; and lipid peroxidation by malondialdehyde (MDA) generation. The reactions after handling were compared with the values measured immediately after blood withdrawal which served as reference values of `genuine' PMN reactivity. The outstanding result was the marked scatter between the individual reactions. Overall, the proportion of migrating PMNs in the blood total decreased, while CL, correlating positively with MDA, increased with the time of storage. EL increased considerably in some of the samples. Agitation raised CL and MDA. The effect of temperature was apparent only after 24 h at 37°C. There was evidence that inhomogeneities in the blood samples were another interfering factor, since resuspension of sedimented blood after storage can be incomplete. In order to obtain reliable results from PMN functional tests, whole-blood assays and processing of blood samples within 20 min after blood withdrawal are recommended.</description><subject>Adult</subject><subject>Blood Preservation</subject><subject>Blood samples</subject><subject>Cell Movement</subject><subject>Cold Temperature</subject><subject>Enzyme Activation</subject><subject>Female</subject><subject>Handling</subject><subject>Humans</subject><subject>Leukocyte Elastase - blood</subject><subject>Luminescent Measurements</subject><subject>Male</subject><subject>Malondialdehyde - blood</subject><subject>Middle Aged</subject><subject>Neutrophils - enzymology</subject><subject>Neutrophils - physiology</subject><subject>Polymorphonuclear leukocyte functions</subject><subject>Storage</subject><subject>Stress, Mechanical</subject><subject>Temperature</subject><subject>Thermal stress</subject><subject>Time Factors</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLxDAUhYMoOj5-gpCVKFhN2knTrEQGXyC4UNchTW5nom1Tk1SZf2_mgVtvFndxz7mXfAehU0quKKHl9SsheZ5RzsS54BeEkIplYgdNaMXzjAvCdtHkT3KADkP4SCJKSrKP9kWRV6wUE_QzW6h-DgHbHscF4MG1y875YeH6UbegPG5h_HR6GQE3Y6-jdT12Da5b5wwOqhvatdmMGgyulzhE59UccLQdXOII3QBexdEDVr3Bam6jWu04RnuNagOcbPsRer-_e5s9Zs8vD0-z2-dMF4LGrMq1Sk-baqoZiJKROlWTF1wzNp0alj5CuVZVXtQ6WUzJSw68rJpKKVrz4gidbfYO3n2NEKLsbNDQtqoHNwbJRZHAFeRfIS0TOUpWQrYRau9C8NDIwdtO-aWkRK6Sketk5Aq7FFyuk5Ei-U63B8a6A_Pn2kaR5jebOSQc3xa8DNpCn7BaDzpK4-w_F34Bs6egTQ</recordid><startdate>19970807</startdate><enddate>19970807</enddate><creator>Egger, Gerd</creator><creator>Kukovetz, Elisabeth M</creator><creator>Hayn, Marianne</creator><creator>Fabjan, Judith S</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19970807</creationdate><title>Changes in the polymorphonuclear leukocyte function of blood samples induced by storage time, temperature and agitation</title><author>Egger, Gerd ; Kukovetz, Elisabeth M ; Hayn, Marianne ; Fabjan, Judith S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-82cacaccd84c5e9650bbbbf237c5544d593217ca823bcc39d6767e768f8aa1b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adult</topic><topic>Blood Preservation</topic><topic>Blood samples</topic><topic>Cell Movement</topic><topic>Cold Temperature</topic><topic>Enzyme Activation</topic><topic>Female</topic><topic>Handling</topic><topic>Humans</topic><topic>Leukocyte Elastase - blood</topic><topic>Luminescent Measurements</topic><topic>Male</topic><topic>Malondialdehyde - blood</topic><topic>Middle Aged</topic><topic>Neutrophils - enzymology</topic><topic>Neutrophils - physiology</topic><topic>Polymorphonuclear leukocyte functions</topic><topic>Storage</topic><topic>Stress, Mechanical</topic><topic>Temperature</topic><topic>Thermal stress</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Egger, Gerd</creatorcontrib><creatorcontrib>Kukovetz, Elisabeth M</creatorcontrib><creatorcontrib>Hayn, Marianne</creatorcontrib><creatorcontrib>Fabjan, Judith S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Egger, Gerd</au><au>Kukovetz, Elisabeth M</au><au>Hayn, Marianne</au><au>Fabjan, Judith S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in the polymorphonuclear leukocyte function of blood samples induced by storage time, temperature and agitation</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1997-08-07</date><risdate>1997</risdate><volume>206</volume><issue>1</issue><spage>61</spage><epage>71</epage><pages>61-71</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><abstract>We have investigated changes in polymorphonuclear leukocyte (PMN) functions of blood samples caused by such typical elements of laboratory handling as storage time, temperature and agitation. The blood of five healthy subjects was stored upright in test tubes at 4, 22 and 37°C over periods of 20 min, one, two, six and 24 h. Controlled agitation was performed on a shaker. The following PMN functional parameters were measured: the white blood cell count (WBC), migration, elastase (EL) release, reactive oxygen species (ROS) production and lipid peroxidation. Migration was determined in a whole-blood membrane filter assay; ROS production by latex-stimulated, luminol-enhanced chemiluminescence (CL) in a whole-blood assay; EL as EL
α1-antitrypsin complex; and lipid peroxidation by malondialdehyde (MDA) generation. The reactions after handling were compared with the values measured immediately after blood withdrawal which served as reference values of `genuine' PMN reactivity. The outstanding result was the marked scatter between the individual reactions. Overall, the proportion of migrating PMNs in the blood total decreased, while CL, correlating positively with MDA, increased with the time of storage. EL increased considerably in some of the samples. Agitation raised CL and MDA. The effect of temperature was apparent only after 24 h at 37°C. There was evidence that inhomogeneities in the blood samples were another interfering factor, since resuspension of sedimented blood after storage can be incomplete. In order to obtain reliable results from PMN functional tests, whole-blood assays and processing of blood samples within 20 min after blood withdrawal are recommended.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9328569</pmid><doi>10.1016/S0022-1759(97)00085-9</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1997-08, Vol.206 (1), p.61-71 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adult Blood Preservation Blood samples Cell Movement Cold Temperature Enzyme Activation Female Handling Humans Leukocyte Elastase - blood Luminescent Measurements Male Malondialdehyde - blood Middle Aged Neutrophils - enzymology Neutrophils - physiology Polymorphonuclear leukocyte functions Storage Stress, Mechanical Temperature Thermal stress Time Factors |
| title | Changes in the polymorphonuclear leukocyte function of blood samples induced by storage time, temperature and agitation |
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