Detection of basal levels and induction of delta opioid receptor mRNA in murine splenocytes
Activation of delta opioid receptors (DOR) modulates calcium mobilization, interleukin-2 production, chemotaxis and proliferation of T-lymphocytes. Recent reports indicate that lymphocytes and mononuclear cells may express mRNA transcripts for DOR. The investigations reported herein show that low le...
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| Veröffentlicht in: | Journal of neuroimmunology 1997-09, Vol.78 (1), p.198-202 |
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| container_title | Journal of neuroimmunology |
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| creator | Sharp, Burt M Shahabi, Nahid McKean, David Li, Ming D McAllen, Kathy |
| description | Activation of delta opioid receptors (DOR) modulates calcium mobilization, interleukin-2 production, chemotaxis and proliferation of T-lymphocytes. Recent reports indicate that lymphocytes and mononuclear cells may express mRNA transcripts for DOR. The investigations reported herein show that low levels of DOR were consistently detected by RT-PCR amplification of RNA from freshly obtained Balb/c murine splenocytes, both weanling and adult. Culturing cells without stimulation increased DOR levels and concanavalin A apparently reduced this; DOR was preferentially expressed in a T-cell-enriched fraction. Thus, the expression of DOR mRNA by unactivated splenocytes is modulated by culture and con A in the T-cell fraction. |
| doi_str_mv | 10.1016/S0165-5728(97)00101-X |
| format | Article |
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Recent reports indicate that lymphocytes and mononuclear cells may express mRNA transcripts for DOR. The investigations reported herein show that low levels of DOR were consistently detected by RT-PCR amplification of RNA from freshly obtained Balb/c murine splenocytes, both weanling and adult. Culturing cells without stimulation increased DOR levels and concanavalin A apparently reduced this; DOR was preferentially expressed in a T-cell-enriched fraction. 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Recent reports indicate that lymphocytes and mononuclear cells may express mRNA transcripts for DOR. The investigations reported herein show that low levels of DOR were consistently detected by RT-PCR amplification of RNA from freshly obtained Balb/c murine splenocytes, both weanling and adult. Culturing cells without stimulation increased DOR levels and concanavalin A apparently reduced this; DOR was preferentially expressed in a T-cell-enriched fraction. 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| identifier | ISSN: 0165-5728 |
| ispartof | Journal of neuroimmunology, 1997-09, Vol.78 (1), p.198-202 |
| issn | 0165-5728 1872-8421 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adult Aging - metabolism Animals Animals, Newborn - metabolism Concanavalin A Female Mice Mice, Inbred BALB C Polymerase Chain Reaction Receptors, Opioid, delta - genetics RNA, Messenger - metabolism RT-PCR Spleen - cytology Spleen - metabolism T-cell T-Lymphocytes - metabolism Transcription, Genetic Weanling |
| title | Detection of basal levels and induction of delta opioid receptor mRNA in murine splenocytes |
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