AN ENZYME LINKED IMMUNOSORPTION ASSAY FOR TISSUE FACTOR PATHWAY INHIBITOR
An assay for the quantification of full-length and carboxy-terminus truncated tissue factor pathway inhibitor (TFPI) has been developed. The assay is a classical two-antibody sandwich assay with a monoclonal capture antibody directed against the third Kunitz-type domain of human TFPI and a polyclona...
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| Veröffentlicht in: | Thrombosis research 1997-09, Vol.87 (5), p.447-459 |
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| container_title | Thrombosis research |
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| creator | Øtergaard, Per B. Beck, Thomas C. Ørsted, Helle Svendsen, Anita Nordfang, Ole Sandset, Per M. Hansen, John-Bjarne |
| description | An assay for the quantification of full-length and carboxy-terminus truncated tissue factor pathway inhibitor (TFPI) has been developed. The assay is a classical two-antibody sandwich assay with a monoclonal capture antibody directed against the third Kunitz-type domain of human TFPI and a polyclonal rabbit peroxidase-labelled anti-human TFPI detecting antibody. The assay is sensitive to full-length and carboxy-terminus truncated TFPI with intact third Kunitz-type domain, but not to two-domain TFPI. TFPI associated with lipoproteins is not or only sparsely detected and TFPI in complex with factor Xa only partially measured. The assay gives linear reference curves in the dose range of 5 to 100 ng/ml in a double logarithmic plot. The normal range assessed from analyses on citrated plasma from 81 normal human donors is 7.8 to 26.0 ng/ml (average ± 2 SD, log-normal distribution). There is no statistically significant difference between TFPI levels measured in 10 fasting and 10 non-fasting individuals. The reproducibility of the assay is about 5.6–5.9% (relative standard error) and the within-days and between-days reproducibilities are 4.7–5.1% and 5.9–8.5%, respectively. The assay is in very good agreement with a commercial ELISA assay recently marketed. A robust, reproducible and convenient ELISA assay for the determination of full-length and three-domain TFPI has been developed. ©
1997 Elsevier Science Ltd |
| doi_str_mv | 10.1016/S0049-3848(97)00161-8 |
| format | Article |
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1997 Elsevier Science Ltd</description><identifier>ISSN: 0049-3848</identifier><identifier>EISSN: 1879-2472</identifier><identifier>DOI: 10.1016/S0049-3848(97)00161-8</identifier><identifier>PMID: 9306619</identifier><identifier>CODEN: THBRAA</identifier><language>eng</language><publisher>New York, NY: Elsevier Ltd</publisher><subject>analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; enzyme linked immunosorption assay ; Enzyme-Linked Immunosorbent Assay - methods ; Enzymes and enzyme inhibitors ; Factor Xa Inhibitors ; Fibrinolytic Agents - blood ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrolases ; Lipoproteins - blood ; Rabbits ; Reagent Kits, Diagnostic ; Reference Values ; Reproducibility of Results ; Sensitivity and Specificity ; Tissue factor pathway inhibitor</subject><ispartof>Thrombosis research, 1997-09, Vol.87 (5), p.447-459</ispartof><rights>1997 Elsevier Science Ltd</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-fab169a9360c415d71a21b3c747b84a2cc9cb6a966e523ba02d6d6b65b7871673</citedby><cites>FETCH-LOGICAL-c389t-fab169a9360c415d71a21b3c747b84a2cc9cb6a966e523ba02d6d6b65b7871673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0049-3848(97)00161-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2819230$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9306619$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Øtergaard, Per B.</creatorcontrib><creatorcontrib>Beck, Thomas C.</creatorcontrib><creatorcontrib>Ørsted, Helle</creatorcontrib><creatorcontrib>Svendsen, Anita</creatorcontrib><creatorcontrib>Nordfang, Ole</creatorcontrib><creatorcontrib>Sandset, Per M.</creatorcontrib><creatorcontrib>Hansen, John-Bjarne</creatorcontrib><title>AN ENZYME LINKED IMMUNOSORPTION ASSAY FOR TISSUE FACTOR PATHWAY INHIBITOR</title><title>Thrombosis research</title><addtitle>Thromb Res</addtitle><description>An assay for the quantification of full-length and carboxy-terminus truncated tissue factor pathway inhibitor (TFPI) has been developed. The assay is a classical two-antibody sandwich assay with a monoclonal capture antibody directed against the third Kunitz-type domain of human TFPI and a polyclonal rabbit peroxidase-labelled anti-human TFPI detecting antibody. The assay is sensitive to full-length and carboxy-terminus truncated TFPI with intact third Kunitz-type domain, but not to two-domain TFPI. TFPI associated with lipoproteins is not or only sparsely detected and TFPI in complex with factor Xa only partially measured. The assay gives linear reference curves in the dose range of 5 to 100 ng/ml in a double logarithmic plot. The normal range assessed from analyses on citrated plasma from 81 normal human donors is 7.8 to 26.0 ng/ml (average ± 2 SD, log-normal distribution). There is no statistically significant difference between TFPI levels measured in 10 fasting and 10 non-fasting individuals. The reproducibility of the assay is about 5.6–5.9% (relative standard error) and the within-days and between-days reproducibilities are 4.7–5.1% and 5.9–8.5%, respectively. The assay is in very good agreement with a commercial ELISA assay recently marketed. A robust, reproducible and convenient ELISA assay for the determination of full-length and three-domain TFPI has been developed. ©
1997 Elsevier Science Ltd</description><subject>analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>enzyme linked immunosorption assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Factor Xa Inhibitors</subject><subject>Fibrinolytic Agents - blood</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrolases</subject><subject>Lipoproteins - blood</subject><subject>Rabbits</subject><subject>Reagent Kits, Diagnostic</subject><subject>Reference Values</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Tissue factor pathway inhibitor</subject><issn>0049-3848</issn><issn>1879-2472</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkFtPwjAYhhujQUR_AskujNGLabtDD1dm4pBG2AgbMXjTdF1JZoDhCib-e8sh3HrV9Huft4cHgC6Cjwgi_JRBGDDXpwG9Z-QB2hFy6RloI0qY6wXEOwftE3IJroz5shBBLGyBFvMhxoi1AY8SJ04-Z6PYGfLkPX51-Gg0TdIsnYxzniZOlGXRzOmnEyfnWTaNnX7Uy-1uHOWDD5vwZMBfuJ1cg4u5XBh9c1w7YNqP897AHaZvvBcNXeVTtnHnskCYSeZjqAIUlgRJDxW-IgEpaCA9pZgqsGQY69DzCwm9Epe4wGFBKEGY-B1wdzh33dTfW202YlkZpRcLudL11ghi_xZS2-2A8ACqpjam0XOxbqqlbH4FgmKnUOwVip0fwYjYKxTU9rrHC7bFUpen1tGZzW-PuTRKLuaNXKnKnDCPIub50GLPB0xbGT-VboRRlV4pXVaNVhtR1tU_D_kD81GF3A</recordid><startdate>19970901</startdate><enddate>19970901</enddate><creator>Øtergaard, Per B.</creator><creator>Beck, Thomas C.</creator><creator>Ørsted, Helle</creator><creator>Svendsen, Anita</creator><creator>Nordfang, Ole</creator><creator>Sandset, Per M.</creator><creator>Hansen, John-Bjarne</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970901</creationdate><title>AN ENZYME LINKED IMMUNOSORPTION ASSAY FOR TISSUE FACTOR PATHWAY INHIBITOR</title><author>Øtergaard, Per B. ; Beck, Thomas C. ; Ørsted, Helle ; Svendsen, Anita ; Nordfang, Ole ; Sandset, Per M. ; Hansen, John-Bjarne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-fab169a9360c415d71a21b3c747b84a2cc9cb6a966e523ba02d6d6b65b7871673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>enzyme linked immunosorption assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Factor Xa Inhibitors</topic><topic>Fibrinolytic Agents - blood</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydrolases</topic><topic>Lipoproteins - blood</topic><topic>Rabbits</topic><topic>Reagent Kits, Diagnostic</topic><topic>Reference Values</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Tissue factor pathway inhibitor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Øtergaard, Per B.</creatorcontrib><creatorcontrib>Beck, Thomas C.</creatorcontrib><creatorcontrib>Ørsted, Helle</creatorcontrib><creatorcontrib>Svendsen, Anita</creatorcontrib><creatorcontrib>Nordfang, Ole</creatorcontrib><creatorcontrib>Sandset, Per M.</creatorcontrib><creatorcontrib>Hansen, John-Bjarne</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Thrombosis research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Øtergaard, Per B.</au><au>Beck, Thomas C.</au><au>Ørsted, Helle</au><au>Svendsen, Anita</au><au>Nordfang, Ole</au><au>Sandset, Per M.</au><au>Hansen, John-Bjarne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AN ENZYME LINKED IMMUNOSORPTION ASSAY FOR TISSUE FACTOR PATHWAY INHIBITOR</atitle><jtitle>Thrombosis research</jtitle><addtitle>Thromb Res</addtitle><date>1997-09-01</date><risdate>1997</risdate><volume>87</volume><issue>5</issue><spage>447</spage><epage>459</epage><pages>447-459</pages><issn>0049-3848</issn><eissn>1879-2472</eissn><coden>THBRAA</coden><abstract>An assay for the quantification of full-length and carboxy-terminus truncated tissue factor pathway inhibitor (TFPI) has been developed. The assay is a classical two-antibody sandwich assay with a monoclonal capture antibody directed against the third Kunitz-type domain of human TFPI and a polyclonal rabbit peroxidase-labelled anti-human TFPI detecting antibody. The assay is sensitive to full-length and carboxy-terminus truncated TFPI with intact third Kunitz-type domain, but not to two-domain TFPI. TFPI associated with lipoproteins is not or only sparsely detected and TFPI in complex with factor Xa only partially measured. The assay gives linear reference curves in the dose range of 5 to 100 ng/ml in a double logarithmic plot. The normal range assessed from analyses on citrated plasma from 81 normal human donors is 7.8 to 26.0 ng/ml (average ± 2 SD, log-normal distribution). There is no statistically significant difference between TFPI levels measured in 10 fasting and 10 non-fasting individuals. The reproducibility of the assay is about 5.6–5.9% (relative standard error) and the within-days and between-days reproducibilities are 4.7–5.1% and 5.9–8.5%, respectively. The assay is in very good agreement with a commercial ELISA assay recently marketed. A robust, reproducible and convenient ELISA assay for the determination of full-length and three-domain TFPI has been developed. ©
1997 Elsevier Science Ltd</abstract><cop>New York, NY</cop><pub>Elsevier Ltd</pub><pmid>9306619</pmid><doi>10.1016/S0049-3848(97)00161-8</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0049-3848 |
| ispartof | Thrombosis research, 1997-09, Vol.87 (5), p.447-459 |
| issn | 0049-3848 1879-2472 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | analysis Analytical, structural and metabolic biochemistry Animals Biological and medical sciences enzyme linked immunosorption assay Enzyme-Linked Immunosorbent Assay - methods Enzymes and enzyme inhibitors Factor Xa Inhibitors Fibrinolytic Agents - blood Fundamental and applied biological sciences. Psychology Humans Hydrolases Lipoproteins - blood Rabbits Reagent Kits, Diagnostic Reference Values Reproducibility of Results Sensitivity and Specificity Tissue factor pathway inhibitor |
| title | AN ENZYME LINKED IMMUNOSORPTION ASSAY FOR TISSUE FACTOR PATHWAY INHIBITOR |
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