Evidence for unusually short tubulin mRNA leaders and characterization of tubulin genes in Giardia lamblia
We have cloned α- and β-tubulin cDNAs from Giardia lamblia and have used these to determine the gene copy number in the organism and to isolate α- and β-tubulin genomic clones. Studies of the gene organization demonstrate that two copies of β-tubulin are linked in a head-to-head configuration. The D...
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| Veröffentlicht in: | Molecular and biochemical parasitology 1989-08, Vol.36 (1), p.87-99 |
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| container_title | Molecular and biochemical parasitology |
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| creator | Kirk-Mason, Karen E. Turner, Mervyn J. Chakraborty, Prasanta R. |
| description | We have cloned α- and β-tubulin cDNAs from
Giardia lamblia and have used these to determine the gene copy number in the organism and to isolate α- and β-tubulin genomic clones. Studies of the gene organization demonstrate that two copies of β-tubulin are linked in a head-to-head configuration. The DNA from these two copies and that from one α-tubulin copy has been sequenced upstream of the translation initiation codon, and analyzed for consensus to typical eukaryotic promoter sequences. Characterization of the α- and β-tubulin mRNAs in this parasite by primer extension and S1 nuclease mapping has revealed an unusually short (6 nucleotides) 5′ untranslated region. |
| doi_str_mv | 10.1016/0166-6851(89)90204-1 |
| format | Article |
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Giardia lamblia and have used these to determine the gene copy number in the organism and to isolate α- and β-tubulin genomic clones. Studies of the gene organization demonstrate that two copies of β-tubulin are linked in a head-to-head configuration. The DNA from these two copies and that from one α-tubulin copy has been sequenced upstream of the translation initiation codon, and analyzed for consensus to typical eukaryotic promoter sequences. Characterization of the α- and β-tubulin mRNAs in this parasite by primer extension and S1 nuclease mapping has revealed an unusually short (6 nucleotides) 5′ untranslated region.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cloning, Molecular</subject><subject>DNA - analysis</subject><subject>DNA - isolation & purification</subject><subject>DNA Probes</subject><subject>Fundamental and applied biological sciences. 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Genome</subject><subject>Giardia - genetics</subject><subject>Giardia lamblia</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>mRNA leader</subject><subject>Multigene Family</subject><subject>Promoter consensus</subject><subject>Protein Sorting Signals - genetics</subject><subject>Restriction Mapping</subject><subject>RNA, Messenger - analysis</subject><subject>Tubulin - genetics</subject><subject>Tubulin cDNA</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtr3TAQhUVpSW-T_oMUtCihXbjRw9ZjEwghTQuhhZCuxVgaJwqynUp2IPn1tXsvd9kuBg3MN2fEOYQcc_aFM65Ol1KVMg3_ZOxnywSrK_6KbLjRorK1MK_JZo-8Je9KeWCMNVqpA3IgDOcLsyEPl08x4OCRdmOm8zCXGVJ6puV-zBOd5nZOcaD9zY9zmhAC5kJhCNTfQwY_YY4vMMVxoGO3h-9wwEKX5ipCDhFogr5NEY7Imw5Swfe795D8-np5e_Gtuv559f3i_LryUoqpaqSWShnBW2gbhqxF1aIVwshaMOV16LQGHbg2ITQabdOh0MKACXXTWWDykJxsdR_z-HvGMrk-Fo8pwYDjXJy2kglj9X9B3tQ1k6JewHoL-jyWkrFzjzn2kJ8dZ27Nwq1Gu9VoZ6z7m4Xjy9qHnf7c9hj2Szvzl_nH3RyKh9RlGHwse0xLvdxfZc62GC6mPUXMrvi4RhZiRj-5MMZ__-MPy2ulXA</recordid><startdate>198908</startdate><enddate>198908</enddate><creator>Kirk-Mason, Karen E.</creator><creator>Turner, Mervyn J.</creator><creator>Chakraborty, Prasanta R.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>198908</creationdate><title>Evidence for unusually short tubulin mRNA leaders and characterization of tubulin genes in Giardia lamblia</title><author>Kirk-Mason, Karen E. ; Turner, Mervyn J. ; Chakraborty, Prasanta R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-537366821bab50e0be6be922834206c7df77a7d178dd57e95fe2728a8d45f9a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cloning, Molecular</topic><topic>DNA - analysis</topic><topic>DNA - isolation & purification</topic><topic>DNA Probes</topic><topic>Fundamental and applied biological sciences. 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| ispartof | Molecular and biochemical parasitology, 1989-08, Vol.36 (1), p.87-99 |
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| subjects | Animals Base Sequence Biological and medical sciences Blotting, Northern Cloning, Molecular DNA - analysis DNA - isolation & purification DNA Probes Fundamental and applied biological sciences. Psychology Gene Amplification Gene copy Genes. Genome Giardia - genetics Giardia lamblia Molecular and cellular biology Molecular genetics Molecular Sequence Data mRNA leader Multigene Family Promoter consensus Protein Sorting Signals - genetics Restriction Mapping RNA, Messenger - analysis Tubulin - genetics Tubulin cDNA |
| title | Evidence for unusually short tubulin mRNA leaders and characterization of tubulin genes in Giardia lamblia |
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