Modulation of nitric oxide production in RAW 264.7 cells by transforming growth factor-beta and interleukin-10: differential effects on free and encapsulated immunomodulator
The mouse macrophage cell line RAW 264.7 can be stimulated to produce nitric oxide (NO) by muramyltripeptide cholesterol included within biodegradable poly(D,L‐lactide) nanocapsules (NC MTP‐Chol). The aim of this work was to determine whether one or both of the cytokines transforming growth factor‐b...
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| Veröffentlicht in: | Journal of leukocyte biology 1997-09, Vol.62 (3), p.374-380 |
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| creator | Seyler, Isabelle Appel, Martine Devissaguet, Jean‐Philippe Legrand, Philippe Barratt, Gillian |
| description | The mouse macrophage cell line RAW 264.7 can be stimulated to produce nitric oxide (NO) by muramyltripeptide cholesterol included within biodegradable poly(D,L‐lactide) nanocapsules (NC MTP‐Chol). The aim of this work was to determine whether one or both of the cytokines transforming growth factor‐beta (TGF‐β) and interleukin‐10 (IL‐10) could be responsible for feedback control seen at high concentrations. Activated RAW 264.7 cells produced TGF‐β1. When exogenous TGF‐β1 was added during stimulation, a dose‐dependent inhibition of NO production was observed when NC MTP‐Chol was used, whereas activation by the soluble muramyl dipeptide (MDP) was not affected. Furthermore, addition of a blocking antibody to TGF‐β arrested the fall in NO production seen at high concentrations of NC MTP‐Chol. Addition of IL‐10 during RAW 264.7 cell activation also reduced NO production; however, in this case, both NC MTP‐Chol and MDP were equally affected. The presence of anti‐IL‐10 antibody during activation significantly increased NO production. J. Leukoc. Biol. 62: 374–380; 1997. |
| doi_str_mv | 10.1002/jlb.62.3.374 |
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The aim of this work was to determine whether one or both of the cytokines transforming growth factor‐beta (TGF‐β) and interleukin‐10 (IL‐10) could be responsible for feedback control seen at high concentrations. Activated RAW 264.7 cells produced TGF‐β1. When exogenous TGF‐β1 was added during stimulation, a dose‐dependent inhibition of NO production was observed when NC MTP‐Chol was used, whereas activation by the soluble muramyl dipeptide (MDP) was not affected. Furthermore, addition of a blocking antibody to TGF‐β arrested the fall in NO production seen at high concentrations of NC MTP‐Chol. Addition of IL‐10 during RAW 264.7 cell activation also reduced NO production; however, in this case, both NC MTP‐Chol and MDP were equally affected. The presence of anti‐IL‐10 antibody during activation significantly increased NO production. J. Leukoc. 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The aim of this work was to determine whether one or both of the cytokines transforming growth factor‐beta (TGF‐β) and interleukin‐10 (IL‐10) could be responsible for feedback control seen at high concentrations. Activated RAW 264.7 cells produced TGF‐β1. When exogenous TGF‐β1 was added during stimulation, a dose‐dependent inhibition of NO production was observed when NC MTP‐Chol was used, whereas activation by the soluble muramyl dipeptide (MDP) was not affected. Furthermore, addition of a blocking antibody to TGF‐β arrested the fall in NO production seen at high concentrations of NC MTP‐Chol. Addition of IL‐10 during RAW 264.7 cell activation also reduced NO production; however, in this case, both NC MTP‐Chol and MDP were equally affected. The presence of anti‐IL‐10 antibody during activation significantly increased NO production. J. Leukoc. Biol. 62: 374–380; 1997.</description><subject>Acetylmuramyl-Alanyl-Isoglutamine - analogs & derivatives</subject><subject>Acetylmuramyl-Alanyl-Isoglutamine - chemistry</subject><subject>Acetylmuramyl-Alanyl-Isoglutamine - pharmacology</subject><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Cholesterol Esters - pharmacology</subject><subject>Enzyme Induction - drug effects</subject><subject>Interleukin-10 - pharmacology</subject><subject>macrophage activation</subject><subject>Macrophage Activation - drug effects</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - metabolism</subject><subject>Mice</subject><subject>muramyl‐dipeptide</subject><subject>nanocapsules</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase - metabolism</subject><subject>NO synthase</subject><subject>Phagocytosis - drug effects</subject><subject>Transforming Growth Factor beta - pharmacology</subject><subject>tumor necrosis factor</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAQjRCobAs3rki-wKlZxnFiN9xKxacWISEQR8uxx7suib3YicL-KP4j3mbVI5xs6X3MzHtF8YzCmgJUr277bs2rNVszUT8oVrRlVyXjgj0sViBqWjY1wOPiPKVbAGAVh7PirGUgQIhV8edzMFOvRhc8CZZ4N0anSfjtDJJ9zJi-g5wnX69_kIrXa0E09n0i3YGMUflkQxyc35JtDPO4I1bpMcSyw1ER5U1Wjhh7nH46X1J4TYyzFiP60ameYP7rMZE8wUbEOwF6rfbpuBNm9TBMPgzLjiE-KR5Z1Sd8enoviu_v3n67-VBuvrz_eHO9KXXdsro0laINrWoLrEFlDCAC02AVWnrVatuJtuW8aZShyNAw0fJOaTQATYemo-yieLn45gh-TZhGObh0PFt5DFOSIucHDeP_JVKeg65ElYmXC1HHkFJEK_fRDSoeJAV5rFHmGiWvJJO5xkx_fvKdugHNPfnUW8bpgs-ux8M_veSnzRtYPF8smp3b7mYXUaZB9X2eUMl5nu9n_wUBZLjO</recordid><startdate>199709</startdate><enddate>199709</enddate><creator>Seyler, Isabelle</creator><creator>Appel, Martine</creator><creator>Devissaguet, Jean‐Philippe</creator><creator>Legrand, Philippe</creator><creator>Barratt, Gillian</creator><general>Society for Leukocyte Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199709</creationdate><title>Modulation of nitric oxide production in RAW 264.7 cells by transforming growth factor-beta and interleukin-10: differential effects on free and encapsulated immunomodulator</title><author>Seyler, Isabelle ; Appel, Martine ; Devissaguet, Jean‐Philippe ; Legrand, Philippe ; Barratt, Gillian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4934-d2a15124f035eadd0ee03c0faef189cfb7996655ad1e3ed3796baced005bedb13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Acetylmuramyl-Alanyl-Isoglutamine - analogs & derivatives</topic><topic>Acetylmuramyl-Alanyl-Isoglutamine - chemistry</topic><topic>Acetylmuramyl-Alanyl-Isoglutamine - pharmacology</topic><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Cholesterol Esters - pharmacology</topic><topic>Enzyme Induction - drug effects</topic><topic>Interleukin-10 - pharmacology</topic><topic>macrophage activation</topic><topic>Macrophage Activation - drug effects</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - metabolism</topic><topic>Mice</topic><topic>muramyl‐dipeptide</topic><topic>nanocapsules</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase - metabolism</topic><topic>NO synthase</topic><topic>Phagocytosis - drug effects</topic><topic>Transforming Growth Factor beta - pharmacology</topic><topic>tumor necrosis factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seyler, Isabelle</creatorcontrib><creatorcontrib>Appel, Martine</creatorcontrib><creatorcontrib>Devissaguet, Jean‐Philippe</creatorcontrib><creatorcontrib>Legrand, Philippe</creatorcontrib><creatorcontrib>Barratt, Gillian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of leukocyte biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seyler, Isabelle</au><au>Appel, Martine</au><au>Devissaguet, Jean‐Philippe</au><au>Legrand, Philippe</au><au>Barratt, Gillian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of nitric oxide production in RAW 264.7 cells by transforming growth factor-beta and interleukin-10: differential effects on free and encapsulated immunomodulator</atitle><jtitle>Journal of leukocyte biology</jtitle><addtitle>J Leukoc Biol</addtitle><date>1997-09</date><risdate>1997</risdate><volume>62</volume><issue>3</issue><spage>374</spage><epage>380</epage><pages>374-380</pages><issn>0741-5400</issn><eissn>1938-3673</eissn><abstract>The mouse macrophage cell line RAW 264.7 can be stimulated to produce nitric oxide (NO) by muramyltripeptide cholesterol included within biodegradable poly(D,L‐lactide) nanocapsules (NC MTP‐Chol). The aim of this work was to determine whether one or both of the cytokines transforming growth factor‐beta (TGF‐β) and interleukin‐10 (IL‐10) could be responsible for feedback control seen at high concentrations. Activated RAW 264.7 cells produced TGF‐β1. When exogenous TGF‐β1 was added during stimulation, a dose‐dependent inhibition of NO production was observed when NC MTP‐Chol was used, whereas activation by the soluble muramyl dipeptide (MDP) was not affected. Furthermore, addition of a blocking antibody to TGF‐β arrested the fall in NO production seen at high concentrations of NC MTP‐Chol. Addition of IL‐10 during RAW 264.7 cell activation also reduced NO production; however, in this case, both NC MTP‐Chol and MDP were equally affected. The presence of anti‐IL‐10 antibody during activation significantly increased NO production. J. Leukoc. Biol. 62: 374–380; 1997.</abstract><cop>United States</cop><pub>Society for Leukocyte Biology</pub><pmid>9307077</pmid><doi>10.1002/jlb.62.3.374</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Acetylmuramyl-Alanyl-Isoglutamine - analogs & derivatives Acetylmuramyl-Alanyl-Isoglutamine - chemistry Acetylmuramyl-Alanyl-Isoglutamine - pharmacology Adjuvants, Immunologic - pharmacology Animals Cell Line Cholesterol Esters - pharmacology Enzyme Induction - drug effects Interleukin-10 - pharmacology macrophage activation Macrophage Activation - drug effects Macrophages - drug effects Macrophages - metabolism Mice muramyl‐dipeptide nanocapsules Nitric Oxide - biosynthesis Nitric Oxide Synthase - metabolism NO synthase Phagocytosis - drug effects Transforming Growth Factor beta - pharmacology tumor necrosis factor |
| title | Modulation of nitric oxide production in RAW 264.7 cells by transforming growth factor-beta and interleukin-10: differential effects on free and encapsulated immunomodulator |
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