Induction of tolerance in rat cortical neurons: hypoxic preconditioning
Sublethal ischemia leads to increased tolerance against subsequent prolonged cerebral ischemia in vivo. In the present study we modeled preconditioning mechanisms in a neuronal-enriched culture. Damage was significantly reduced (up to 72%) with 1.5 h of oxygen-glucose deprivation 48–72 h before 3 h...
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| Veröffentlicht in: | FEBS letters 1997-09, Vol.414 (1), p.117-121 |
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| container_title | FEBS letters |
| container_volume | 414 |
| creator | Bruer, Ulrike Weih, Markus K Isaev, Nikolaj K Meisel, Andreas Ruscher, Karsten Bergk, Alexandra Trendelenburg, George Wiegand, Frank Victorov, Ilya V Dirnagl, Ulrich |
| description | Sublethal ischemia leads to increased tolerance against subsequent prolonged cerebral ischemia in vivo. In the present study we modeled preconditioning mechanisms in a neuronal-enriched culture. Damage was significantly reduced (up to 72%) with 1.5 h of oxygen-glucose deprivation 48–72 h before 3 h oxygen-glucose deprivation. Tolerance was also elicited by Na
+-K
+-ATPase inhibition. No damage was observed when astroglial or endothelial cells were exposed to hypoxia for 3 and 6 h, respectively. We conclude that hypoxic preconditioning is a robust neuronal phenomenon in vitro with a similar temporal pattern and selective cellular vulnerability as the ischemic tolerance phenomenon shown in vivo. |
| doi_str_mv | 10.1016/S0014-5793(97)00954-X |
| format | Article |
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+-K
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+-K
+-ATPase inhibition. No damage was observed when astroglial or endothelial cells were exposed to hypoxia for 3 and 6 h, respectively. We conclude that hypoxic preconditioning is a robust neuronal phenomenon in vitro with a similar temporal pattern and selective cellular vulnerability as the ischemic tolerance phenomenon shown in vivo.</description><subject>Animals</subject><subject>Brain Ischemia - metabolism</subject><subject>Cell culture</subject><subject>Cell Hypoxia</subject><subject>Cells, Cultured</subject><subject>Disease Models, Animal</subject><subject>DNA Fragmentation</subject><subject>Electrophoresis, Agar Gel</subject><subject>Female</subject><subject>Glucose - metabolism</subject><subject>Ischemia</subject><subject>Ischemic Preconditioning</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Microscopy, Phase-Contrast</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Ouabain</subject><subject>Ouabain - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors</subject><subject>Stroke</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtOGzEUhi1URMPlEZBmVZXFFDv2jO1uqoIgQUJiAUjZWb6cgKuJndozbfP2eJKIbVlZ9n85Ph9C5wR_I5i0l48YE1Y3XNKvkl9gLBtWLw7QhAhOa8pa8QlN3i2f0XHOv3C5CyKP0JGkuOGMTtDsLrjB9j6GKi6rPnaQdLBQ-VAl3Vc2pt5b3VUBhhRD_l69btbxn7fVOoGNwfkx6sPLKTpc6i7D2f48Qc-3N0_X8_r-YXZ3_fO-tg0Vi7o1VIBhjlMiWmMwZszItuVUgjHWMianWi-Fc8aCa1uDNZemLMAB8BSA0BP0Zde7TvH3ALlXK58tdJ0OEIesyq6lVIhibHZGm2LOCZZqnfxKp40iWI0A1RagGukoydUWoFqU3Pl-wGBW4N5Te2JFn-_0v76DzcdK1e3N1XSrjILk2-dx1I9dFRRgfzwkla2HQt_5ArdXLvr_fPYNbduVtw</recordid><startdate>19970901</startdate><enddate>19970901</enddate><creator>Bruer, Ulrike</creator><creator>Weih, Markus K</creator><creator>Isaev, Nikolaj K</creator><creator>Meisel, Andreas</creator><creator>Ruscher, Karsten</creator><creator>Bergk, Alexandra</creator><creator>Trendelenburg, George</creator><creator>Wiegand, Frank</creator><creator>Victorov, Ilya V</creator><creator>Dirnagl, Ulrich</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970901</creationdate><title>Induction of tolerance in rat cortical neurons: hypoxic preconditioning</title><author>Bruer, Ulrike ; Weih, Markus K ; Isaev, Nikolaj K ; Meisel, Andreas ; Ruscher, Karsten ; Bergk, Alexandra ; Trendelenburg, George ; Wiegand, Frank ; Victorov, Ilya V ; Dirnagl, Ulrich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538X-6b38eb4d73186bb0044b966739ebbcc4492aaf8ddbced66b0a79b5797ee02ee13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Brain Ischemia - metabolism</topic><topic>Cell culture</topic><topic>Cell Hypoxia</topic><topic>Cells, Cultured</topic><topic>Disease Models, Animal</topic><topic>DNA Fragmentation</topic><topic>Electrophoresis, Agar Gel</topic><topic>Female</topic><topic>Glucose - metabolism</topic><topic>Ischemia</topic><topic>Ischemic Preconditioning</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Microscopy, Phase-Contrast</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Ouabain</topic><topic>Ouabain - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors</topic><topic>Stroke</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bruer, Ulrike</creatorcontrib><creatorcontrib>Weih, Markus K</creatorcontrib><creatorcontrib>Isaev, Nikolaj K</creatorcontrib><creatorcontrib>Meisel, Andreas</creatorcontrib><creatorcontrib>Ruscher, Karsten</creatorcontrib><creatorcontrib>Bergk, Alexandra</creatorcontrib><creatorcontrib>Trendelenburg, George</creatorcontrib><creatorcontrib>Wiegand, Frank</creatorcontrib><creatorcontrib>Victorov, Ilya V</creatorcontrib><creatorcontrib>Dirnagl, Ulrich</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bruer, Ulrike</au><au>Weih, Markus K</au><au>Isaev, Nikolaj K</au><au>Meisel, Andreas</au><au>Ruscher, Karsten</au><au>Bergk, Alexandra</au><au>Trendelenburg, George</au><au>Wiegand, Frank</au><au>Victorov, Ilya V</au><au>Dirnagl, Ulrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of tolerance in rat cortical neurons: hypoxic preconditioning</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1997-09-01</date><risdate>1997</risdate><volume>414</volume><issue>1</issue><spage>117</spage><epage>121</epage><pages>117-121</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Sublethal ischemia leads to increased tolerance against subsequent prolonged cerebral ischemia in vivo. In the present study we modeled preconditioning mechanisms in a neuronal-enriched culture. Damage was significantly reduced (up to 72%) with 1.5 h of oxygen-glucose deprivation 48–72 h before 3 h oxygen-glucose deprivation. Tolerance was also elicited by Na
+-K
+-ATPase inhibition. No damage was observed when astroglial or endothelial cells were exposed to hypoxia for 3 and 6 h, respectively. We conclude that hypoxic preconditioning is a robust neuronal phenomenon in vitro with a similar temporal pattern and selective cellular vulnerability as the ischemic tolerance phenomenon shown in vivo.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>9305743</pmid><doi>10.1016/S0014-5793(97)00954-X</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Free Content; Access via Wiley Online Library; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Brain Ischemia - metabolism Cell culture Cell Hypoxia Cells, Cultured Disease Models, Animal DNA Fragmentation Electrophoresis, Agar Gel Female Glucose - metabolism Ischemia Ischemic Preconditioning L-Lactate Dehydrogenase - metabolism Microscopy, Phase-Contrast Neurons - cytology Neurons - metabolism Ouabain Ouabain - pharmacology Rats Rats, Wistar Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors Stroke |
| title | Induction of tolerance in rat cortical neurons: hypoxic preconditioning |
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