Human serotonin 5‐HT7 receptor: cloning and pharmacological characterisation of two receptor variants
Two splice variants of the 5‐HT7 receptor were identified in human brain that differ in the lengths of their intracellular carboxy terminal tail. Identification of the variants of this receptor is of particular interest since the 5‐HT7 receptor is known to have a high affinity for a number of antide...
Gespeichert in:
| Veröffentlicht in: | FEBS letters 1997-08, Vol.413 (3), p.489-494 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 494 |
|---|---|
| container_issue | 3 |
| container_start_page | 489 |
| container_title | FEBS letters |
| container_volume | 413 |
| creator | Stam, Nico J Roesink, Carolien Dijcks, Fred Garritsen, Anja van Herpen, Anne Olijve, Wiebe |
| description | Two splice variants of the 5‐HT7 receptor were identified in human brain that differ in the lengths of their intracellular carboxy terminal tail. Identification of the variants of this receptor is of particular interest since the 5‐HT7 receptor is known to have a high affinity for a number of antidepressants and is localized in brain regions thought to be implicated in depression. The two isoforms are expressed in roughly equal amounts in various regions of the human brain. When expressed in NIH‐3T3 cells, both variants encode functional 5‐HT7 receptors, positively coupled to adenylyl cyclase. We suggest that both variants are derived from a single gene by alternative mRNA splicing. Furthermore, our results from Southern blot analysis studies suggest that additional 5‐HT7 receptor genes may exist in human. |
| doi_str_mv | 10.1016/S0014-5793(97)00964-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_79299990</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79299990</sourcerecordid><originalsourceid>FETCH-LOGICAL-p2712-2f5ea5b07ae3fa6c75ee99b65cb829e66c2a213c9dcaf154738c5fa7afe4e5933</originalsourceid><addsrcrecordid>eNo9kEtOwzAQhi0EgvI4ApJXCBYBP-I4ZgcVUCQkFsDamrqTEpTEwU6puuMInJGTkJSqsxnNfP_M4iPklLNLznh29cIYTxOljTw3-oIxk6WJ2CEjnmuZyDTLd8loGzkghzF-sH7Oudkn-0YyqTI-IvPJooaGRgy-803ZUPX7_TN51TSgw7bz4Zq6agBzCs2Mtu8QanC-8vPSQUVdP4PrMJQRutI31Be0W_rtNf2CUELTxWOyV0AV8WTTj8jb_d3reJI8PT88jm-eklZoLhJRKAQ1ZRpQFpA5rRCNmWbKTXNhMMucAMGlMzMHBVeplrlTBWgoMEVlpDwiZ_9_2-A_Fxg7W5fRYVVBg34RrTbC9MX64OkmuJjWOLNtKGsIK7sx0_PJP1-WFa62mDM72Ldr-3ZQa422a_tW2Pu7W7EmAzB6vRbyD61rfW0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79299990</pqid></control><display><type>article</type><title>Human serotonin 5‐HT7 receptor: cloning and pharmacological characterisation of two receptor variants</title><source>Wiley Free Content</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elsevier ScienceDirect Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Stam, Nico J ; Roesink, Carolien ; Dijcks, Fred ; Garritsen, Anja ; van Herpen, Anne ; Olijve, Wiebe</creator><creatorcontrib>Stam, Nico J ; Roesink, Carolien ; Dijcks, Fred ; Garritsen, Anja ; van Herpen, Anne ; Olijve, Wiebe</creatorcontrib><description>Two splice variants of the 5‐HT7 receptor were identified in human brain that differ in the lengths of their intracellular carboxy terminal tail. Identification of the variants of this receptor is of particular interest since the 5‐HT7 receptor is known to have a high affinity for a number of antidepressants and is localized in brain regions thought to be implicated in depression. The two isoforms are expressed in roughly equal amounts in various regions of the human brain. When expressed in NIH‐3T3 cells, both variants encode functional 5‐HT7 receptors, positively coupled to adenylyl cyclase. We suggest that both variants are derived from a single gene by alternative mRNA splicing. Furthermore, our results from Southern blot analysis studies suggest that additional 5‐HT7 receptor genes may exist in human.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(97)00964-2</identifier><identifier>PMID: 9303561</identifier><language>eng</language><publisher>England</publisher><subject>1-Methyl-3-isobutylxanthine - pharmacology ; 3T3 Cells ; 5-HT ; Alternative Splicing ; Amino Acid Sequence ; Animals ; Base Sequence ; Brain - metabolism ; Cell Membrane - metabolism ; Cloning, Molecular ; Cyclic AMP - metabolism ; Fetus ; G-protein-coupled receptor ; Genetic Variation ; Humans ; Kinetics ; Liver - metabolism ; Mice ; Molecular Sequence Data ; Organ Specificity ; Radioligand Assay ; Receptor variant ; Receptors, Serotonin - biosynthesis ; Receptors, Serotonin - chemistry ; Receptors, Serotonin - physiology ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - chemistry ; Recombinant Proteins - metabolism ; Serotonin - pharmacology ; Thymus Gland - metabolism ; Transfection</subject><ispartof>FEBS letters, 1997-08, Vol.413 (3), p.489-494</ispartof><rights>FEBS Letters 413 (1997) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2897%2900964-2$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0014-5793%2897%2900964-2$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9303561$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stam, Nico J</creatorcontrib><creatorcontrib>Roesink, Carolien</creatorcontrib><creatorcontrib>Dijcks, Fred</creatorcontrib><creatorcontrib>Garritsen, Anja</creatorcontrib><creatorcontrib>van Herpen, Anne</creatorcontrib><creatorcontrib>Olijve, Wiebe</creatorcontrib><title>Human serotonin 5‐HT7 receptor: cloning and pharmacological characterisation of two receptor variants</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Two splice variants of the 5‐HT7 receptor were identified in human brain that differ in the lengths of their intracellular carboxy terminal tail. Identification of the variants of this receptor is of particular interest since the 5‐HT7 receptor is known to have a high affinity for a number of antidepressants and is localized in brain regions thought to be implicated in depression. The two isoforms are expressed in roughly equal amounts in various regions of the human brain. When expressed in NIH‐3T3 cells, both variants encode functional 5‐HT7 receptors, positively coupled to adenylyl cyclase. We suggest that both variants are derived from a single gene by alternative mRNA splicing. Furthermore, our results from Southern blot analysis studies suggest that additional 5‐HT7 receptor genes may exist in human.</description><subject>1-Methyl-3-isobutylxanthine - pharmacology</subject><subject>3T3 Cells</subject><subject>5-HT</subject><subject>Alternative Splicing</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Brain - metabolism</subject><subject>Cell Membrane - metabolism</subject><subject>Cloning, Molecular</subject><subject>Cyclic AMP - metabolism</subject><subject>Fetus</subject><subject>G-protein-coupled receptor</subject><subject>Genetic Variation</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Liver - metabolism</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Organ Specificity</subject><subject>Radioligand Assay</subject><subject>Receptor variant</subject><subject>Receptors, Serotonin - biosynthesis</subject><subject>Receptors, Serotonin - chemistry</subject><subject>Receptors, Serotonin - physiology</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - metabolism</subject><subject>Serotonin - pharmacology</subject><subject>Thymus Gland - metabolism</subject><subject>Transfection</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtOwzAQhi0EgvI4ApJXCBYBP-I4ZgcVUCQkFsDamrqTEpTEwU6puuMInJGTkJSqsxnNfP_M4iPklLNLznh29cIYTxOljTw3-oIxk6WJ2CEjnmuZyDTLd8loGzkghzF-sH7Oudkn-0YyqTI-IvPJooaGRgy-803ZUPX7_TN51TSgw7bz4Zq6agBzCs2Mtu8QanC-8vPSQUVdP4PrMJQRutI31Be0W_rtNf2CUELTxWOyV0AV8WTTj8jb_d3reJI8PT88jm-eklZoLhJRKAQ1ZRpQFpA5rRCNmWbKTXNhMMucAMGlMzMHBVeplrlTBWgoMEVlpDwiZ_9_2-A_Fxg7W5fRYVVBg34RrTbC9MX64OkmuJjWOLNtKGsIK7sx0_PJP1-WFa62mDM72Ldr-3ZQa422a_tW2Pu7W7EmAzB6vRbyD61rfW0</recordid><startdate>19970825</startdate><enddate>19970825</enddate><creator>Stam, Nico J</creator><creator>Roesink, Carolien</creator><creator>Dijcks, Fred</creator><creator>Garritsen, Anja</creator><creator>van Herpen, Anne</creator><creator>Olijve, Wiebe</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19970825</creationdate><title>Human serotonin 5‐HT7 receptor: cloning and pharmacological characterisation of two receptor variants</title><author>Stam, Nico J ; Roesink, Carolien ; Dijcks, Fred ; Garritsen, Anja ; van Herpen, Anne ; Olijve, Wiebe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2712-2f5ea5b07ae3fa6c75ee99b65cb829e66c2a213c9dcaf154738c5fa7afe4e5933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>1-Methyl-3-isobutylxanthine - pharmacology</topic><topic>3T3 Cells</topic><topic>5-HT</topic><topic>Alternative Splicing</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Brain - metabolism</topic><topic>Cell Membrane - metabolism</topic><topic>Cloning, Molecular</topic><topic>Cyclic AMP - metabolism</topic><topic>Fetus</topic><topic>G-protein-coupled receptor</topic><topic>Genetic Variation</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Liver - metabolism</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Organ Specificity</topic><topic>Radioligand Assay</topic><topic>Receptor variant</topic><topic>Receptors, Serotonin - biosynthesis</topic><topic>Receptors, Serotonin - chemistry</topic><topic>Receptors, Serotonin - physiology</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - chemistry</topic><topic>Recombinant Proteins - metabolism</topic><topic>Serotonin - pharmacology</topic><topic>Thymus Gland - metabolism</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stam, Nico J</creatorcontrib><creatorcontrib>Roesink, Carolien</creatorcontrib><creatorcontrib>Dijcks, Fred</creatorcontrib><creatorcontrib>Garritsen, Anja</creatorcontrib><creatorcontrib>van Herpen, Anne</creatorcontrib><creatorcontrib>Olijve, Wiebe</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stam, Nico J</au><au>Roesink, Carolien</au><au>Dijcks, Fred</au><au>Garritsen, Anja</au><au>van Herpen, Anne</au><au>Olijve, Wiebe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human serotonin 5‐HT7 receptor: cloning and pharmacological characterisation of two receptor variants</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1997-08-25</date><risdate>1997</risdate><volume>413</volume><issue>3</issue><spage>489</spage><epage>494</epage><pages>489-494</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Two splice variants of the 5‐HT7 receptor were identified in human brain that differ in the lengths of their intracellular carboxy terminal tail. Identification of the variants of this receptor is of particular interest since the 5‐HT7 receptor is known to have a high affinity for a number of antidepressants and is localized in brain regions thought to be implicated in depression. The two isoforms are expressed in roughly equal amounts in various regions of the human brain. When expressed in NIH‐3T3 cells, both variants encode functional 5‐HT7 receptors, positively coupled to adenylyl cyclase. We suggest that both variants are derived from a single gene by alternative mRNA splicing. Furthermore, our results from Southern blot analysis studies suggest that additional 5‐HT7 receptor genes may exist in human.</abstract><cop>England</cop><pmid>9303561</pmid><doi>10.1016/S0014-5793(97)00964-2</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1997-08, Vol.413 (3), p.489-494 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79299990 |
| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 1-Methyl-3-isobutylxanthine - pharmacology 3T3 Cells 5-HT Alternative Splicing Amino Acid Sequence Animals Base Sequence Brain - metabolism Cell Membrane - metabolism Cloning, Molecular Cyclic AMP - metabolism Fetus G-protein-coupled receptor Genetic Variation Humans Kinetics Liver - metabolism Mice Molecular Sequence Data Organ Specificity Radioligand Assay Receptor variant Receptors, Serotonin - biosynthesis Receptors, Serotonin - chemistry Receptors, Serotonin - physiology Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - metabolism Serotonin - pharmacology Thymus Gland - metabolism Transfection |
| title | Human serotonin 5‐HT7 receptor: cloning and pharmacological characterisation of two receptor variants |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T10%3A17%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20serotonin%205%E2%80%90HT7%20receptor:%20cloning%20and%20pharmacological%20characterisation%20of%20two%20receptor%20variants&rft.jtitle=FEBS%20letters&rft.au=Stam,%20Nico%20J&rft.date=1997-08-25&rft.volume=413&rft.issue=3&rft.spage=489&rft.epage=494&rft.pages=489-494&rft.issn=0014-5793&rft.eissn=1873-3468&rft_id=info:doi/10.1016/S0014-5793(97)00964-2&rft_dat=%3Cproquest_pubme%3E79299990%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79299990&rft_id=info:pmid/9303561&rfr_iscdi=true |