A method for the relative quantification of lipofuscin based on a computer image analysis system
The accumulation of lipofuscin in neurons is the best recognized microscopic alteration of the aging brain. The broad spectrum which characterizes this progressive accumulation of pigment has made necessary the use of quantitative methods for its assessment. Present day techniques for the quantitati...
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| Veröffentlicht in: | Journal of neuroscience methods 1989-10, Vol.30 (1), p.11-15 |
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| creator | Casanova, Manuel F. Koliatsos, Vassilis Jengeleski, Cynthia A. |
| description | The accumulation of lipofuscin in neurons is the best recognized microscopic alteration of the aging brain. The broad spectrum which characterizes this progressive accumulation of pigment has made necessary the use of quantitative methods for its assessment. Present day techniques for the quantitation of lipofuscin (e.g. fluorescent microspectrophotometry and planimetry) are cumbersome, rely on manual techniques and may be limited by fluorescent artifacts. In this article we report a computer image analysis method for the relative quantification of lipofuscin in slides stained with a modified aldehyde fuchsin stain. The method preserves the topography of the pigment, is not limited by artifacts due to fluorescence, and has excellent inter-rater reliability. |
| doi_str_mv | 10.1016/0165-0270(89)90068-X |
| format | Article |
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The broad spectrum which characterizes this progressive accumulation of pigment has made necessary the use of quantitative methods for its assessment. Present day techniques for the quantitation of lipofuscin (e.g. fluorescent microspectrophotometry and planimetry) are cumbersome, rely on manual techniques and may be limited by fluorescent artifacts. In this article we report a computer image analysis method for the relative quantification of lipofuscin in slides stained with a modified aldehyde fuchsin stain. 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The broad spectrum which characterizes this progressive accumulation of pigment has made necessary the use of quantitative methods for its assessment. Present day techniques for the quantitation of lipofuscin (e.g. fluorescent microspectrophotometry and planimetry) are cumbersome, rely on manual techniques and may be limited by fluorescent artifacts. In this article we report a computer image analysis method for the relative quantification of lipofuscin in slides stained with a modified aldehyde fuchsin stain. The method preserves the topography of the pigment, is not limited by artifacts due to fluorescence, and has excellent inter-rater reliability.</description><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain - pathology</subject><subject>Central nervous system</subject><subject>Ceroid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hippocampus - pathology</subject><subject>Histological Techniques</subject><subject>Humans</subject><subject>Image analysis</subject><subject>Image Processing, Computer-Assisted - methods</subject><subject>Lipofuscin</subject><subject>Lipofuscin - analysis</subject><subject>Medulla Oblongata - pathology</subject><subject>Pigments, Biological - analysis</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFrHCEUgKU0pNu0_yAFD6U0h2nVcRy9BEJo00CglwT2Zh19JpaZcaNOYP993e6yx_Yg4nvfe_i-h9A5JV8ooeJrPV1DWE8-S3WhCBGyWb9CKyp71oherl-j1RF5g97m_JsQwhURp-iUCclqfIV-XeEJylN02MeEyxPgBKMp4QXw82LmEnyw9RlnHD0ewyb6Jdsw48FkcLiGDbZx2iwFEg6TeQRsZjNuc8g4b3OB6R068WbM8P5wn6GH79_ur380dz9vbq-v7hrLaV8a1onOM67AeJAtb1vwnLZcDa2w0DpvmHOOUyOdMtSSjtHBDFQQKUAo7ml7hj7t-25SfF4gFz2FbGEczQxxybpXTKlOsP-CtON9vwf5HrQp5pzA602qI6atpkTvNqB3evVOr5ZK_92AXteyD4f-yzCBOxYdlNf8x0PeZGtGn8xsQz5iopNciN08l3sMqrSXAElX7zBbcCGBLdrF8O9__AGHEaLT</recordid><startdate>19891001</startdate><enddate>19891001</enddate><creator>Casanova, Manuel F.</creator><creator>Koliatsos, Vassilis</creator><creator>Jengeleski, Cynthia A.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19891001</creationdate><title>A method for the relative quantification of lipofuscin based on a computer image analysis system</title><author>Casanova, Manuel F. ; Koliatsos, Vassilis ; Jengeleski, Cynthia A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-2565f249eafe83433ef41349b36ce3dfa2ddd41a8d9a1c0521bab16086e694f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain - pathology</topic><topic>Central nervous system</topic><topic>Ceroid</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hippocampus - pathology</topic><topic>Histological Techniques</topic><topic>Humans</topic><topic>Image analysis</topic><topic>Image Processing, Computer-Assisted - methods</topic><topic>Lipofuscin</topic><topic>Lipofuscin - analysis</topic><topic>Medulla Oblongata - pathology</topic><topic>Pigments, Biological - analysis</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casanova, Manuel F.</creatorcontrib><creatorcontrib>Koliatsos, Vassilis</creatorcontrib><creatorcontrib>Jengeleski, Cynthia A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Casanova, Manuel F.</au><au>Koliatsos, Vassilis</au><au>Jengeleski, Cynthia A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A method for the relative quantification of lipofuscin based on a computer image analysis system</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>1989-10-01</date><risdate>1989</risdate><volume>30</volume><issue>1</issue><spage>11</spage><epage>15</epage><pages>11-15</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>The accumulation of lipofuscin in neurons is the best recognized microscopic alteration of the aging brain. The broad spectrum which characterizes this progressive accumulation of pigment has made necessary the use of quantitative methods for its assessment. Present day techniques for the quantitation of lipofuscin (e.g. fluorescent microspectrophotometry and planimetry) are cumbersome, rely on manual techniques and may be limited by fluorescent artifacts. In this article we report a computer image analysis method for the relative quantification of lipofuscin in slides stained with a modified aldehyde fuchsin stain. The method preserves the topography of the pigment, is not limited by artifacts due to fluorescence, and has excellent inter-rater reliability.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2682027</pmid><doi>10.1016/0165-0270(89)90068-X</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of neuroscience methods, 1989-10, Vol.30 (1), p.11-15 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biochemistry and metabolism Biological and medical sciences Brain - pathology Central nervous system Ceroid Fundamental and applied biological sciences. Psychology Hippocampus - pathology Histological Techniques Humans Image analysis Image Processing, Computer-Assisted - methods Lipofuscin Lipofuscin - analysis Medulla Oblongata - pathology Pigments, Biological - analysis Vertebrates: nervous system and sense organs |
| title | A method for the relative quantification of lipofuscin based on a computer image analysis system |
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