Induction of Cytochrome P-450c and P-450d by Metyrapone in the Primary Culture of Rat Hepatocytes
The effects of metyrapone on qualitative changes in cytochrome P-450-dependent drug metabolizing activities in primary cultures of rat hepatocytes were investigated. Metyrapone apparently increased benzo(a)pyrene hydroxylation and maintained both ethoxycoumarin-O-deethylation and propoxycoumarin-O-d...
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| Veröffentlicht in: | Japanese Journal of Pharmacology 1989, Vol.51 (1), p.136-139 |
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| container_title | Japanese Journal of Pharmacology |
| container_volume | 51 |
| creator | HIROTA, Kouichi KAWANISHI, Toru SUNOUCHI, Momoko OHNO, Yasuo TAKANAKA, Akira YAMAZOE, Yasushi KATO, Ryuichi MURAKOSHI, Yoshie |
| description | The effects of metyrapone on qualitative changes in cytochrome P-450-dependent drug metabolizing activities in primary cultures of rat hepatocytes were investigated. Metyrapone apparently increased benzo(a)pyrene hydroxylation and maintained both ethoxycoumarin-O-deethylation and propoxycoumarin-O-depro-pylation. whereas it had little effect on methoxycoumarin-O-demethylation Furthermore, P-450d (high spin type of P-448) as well as P-450c (low spin type of P-448) were induced by metyrapone. while P-450b and P-450e were not. In conclusion, metyrapone act as a 3-methylcholanthrene-like inducer in the primary cultures of rat hepatocytes |
| doi_str_mv | 10.1016/S0021-5198(19)40148-0 |
| format | Article |
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Metyrapone apparently increased benzo(a)pyrene hydroxylation and maintained both ethoxycoumarin-O-deethylation and propoxycoumarin-O-depro-pylation. whereas it had little effect on methoxycoumarin-O-demethylation Furthermore, P-450d (high spin type of P-448) as well as P-450c (low spin type of P-448) were induced by metyrapone. while P-450b and P-450e were not. In conclusion, metyrapone act as a 3-methylcholanthrene-like inducer in the primary cultures of rat hepatocytes</description><identifier>ISSN: 0021-5198</identifier><identifier>EISSN: 1347-3506</identifier><identifier>DOI: 10.1016/S0021-5198(19)40148-0</identifier><identifier>PMID: 2810935</identifier><identifier>CODEN: JJPAAZ</identifier><language>eng</language><publisher>Kyoto: The Japanese Pharmacological Society</publisher><subject>Animals ; Biological and medical sciences ; Cells, Cultured ; Cytochrome P-450 Enzyme System - biosynthesis ; In Vitro Techniques ; Isoenzymes - antagonists & inhibitors ; Isoenzymes - biosynthesis ; Isoenzymes - metabolism ; Liver - drug effects ; Liver - enzymology ; Male ; Medical sciences ; Metyrapone - pharmacology ; Miscellaneous ; Mixed Function Oxygenases - antagonists & inhibitors ; Mixed Function Oxygenases - metabolism ; Pharmacology. Drug treatments ; Phenobarbital - pharmacology ; Rats ; Rats, Inbred Strains</subject><ispartof>Japanese Journal of Pharmacology, 1989, Vol.51 (1), p.136-139</ispartof><rights>1989 Elsevier B.V.</rights><rights>1990 INIST-CNRS</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3380-d3a37a383d1e616da2d56c646c89381c3ad211c625ce9b3133548a97a5734f6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6633782$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2810935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HIROTA, Kouichi</creatorcontrib><creatorcontrib>KAWANISHI, Toru</creatorcontrib><creatorcontrib>SUNOUCHI, Momoko</creatorcontrib><creatorcontrib>OHNO, Yasuo</creatorcontrib><creatorcontrib>TAKANAKA, Akira</creatorcontrib><creatorcontrib>YAMAZOE, Yasushi</creatorcontrib><creatorcontrib>KATO, Ryuichi</creatorcontrib><creatorcontrib>MURAKOSHI, Yoshie</creatorcontrib><creatorcontrib>School of Medicine</creatorcontrib><creatorcontrib>Department of Pharmacology</creatorcontrib><creatorcontrib>National Institute of Hygienic Sciences</creatorcontrib><creatorcontrib>College of Science and Technology</creatorcontrib><creatorcontrib>Department of Pharmacy</creatorcontrib><creatorcontrib>Nihon University</creatorcontrib><creatorcontrib>Keio University</creatorcontrib><title>Induction of Cytochrome P-450c and P-450d by Metyrapone in the Primary Culture of Rat Hepatocytes</title><title>Japanese Journal of Pharmacology</title><addtitle>Jpn J Pharmacol</addtitle><description>The effects of metyrapone on qualitative changes in cytochrome P-450-dependent drug metabolizing activities in primary cultures of rat hepatocytes were investigated. Metyrapone apparently increased benzo(a)pyrene hydroxylation and maintained both ethoxycoumarin-O-deethylation and propoxycoumarin-O-depro-pylation. whereas it had little effect on methoxycoumarin-O-demethylation Furthermore, P-450d (high spin type of P-448) as well as P-450c (low spin type of P-448) were induced by metyrapone. while P-450b and P-450e were not. In conclusion, metyrapone act as a 3-methylcholanthrene-like inducer in the primary cultures of rat hepatocytes</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Cytochrome P-450 Enzyme System - biosynthesis</subject><subject>In Vitro Techniques</subject><subject>Isoenzymes - antagonists & inhibitors</subject><subject>Isoenzymes - biosynthesis</subject><subject>Isoenzymes - metabolism</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metyrapone - pharmacology</subject><subject>Miscellaneous</subject><subject>Mixed Function Oxygenases - antagonists & inhibitors</subject><subject>Mixed Function Oxygenases - metabolism</subject><subject>Pharmacology. Drug treatments</subject><subject>Phenobarbital - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><issn>0021-5198</issn><issn>1347-3506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv3CAURlHVKp0m_QmRWFRVu3ALvgbDqqpGzUNK1apJ1ogBrDDygAt2Jf_74ng022x46B6-yz0IXVLyhRLKv94TUtOKUSk-Ufm5IbQRFXmFNhSatgJG-Gu0OSFv0buc9-UqCneGzmpBiQS2Qfo22MmMPgYcO7ydx2ieUjw4_LtqGDFYB7seLd7N-Kcb56SHGBz2AY9PBUv-oNOMt1M_TsktIX_0iG_coEvUPLp8gd50us_u_XE_R49XPx62N9Xdr-vb7fe7ygAIUlnQ0GoQYKnjlFtdW8YNb7gREgQ1oG1NqeE1M07ugAKwRmjZatZC03EL5-jjmjuk-HdyeVQHn43rex1cnLJqZS1FI0kB2QqaFHNOrlPDOoSiRC1q1bNatXhTVKpntWp5d3lsMO0Ozp5eHV2W-odjXWej-y7pYHw-YZwDtKIu2NWKlQxfuBh6H5zaxymF4keZju_3cehLayEVIax8SRFafkCBL4uEFtqWl6Bva5ArVv95l1Q23gVTYpMzo7LRvzDRf-hjqKA</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>HIROTA, Kouichi</creator><creator>KAWANISHI, Toru</creator><creator>SUNOUCHI, Momoko</creator><creator>OHNO, Yasuo</creator><creator>TAKANAKA, Akira</creator><creator>YAMAZOE, Yasushi</creator><creator>KATO, Ryuichi</creator><creator>MURAKOSHI, Yoshie</creator><general>The Japanese Pharmacological Society</general><general>Japanese Pharmacological Society</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>Induction of Cytochrome P-450c and P-450d by Metyrapone in the Primary Culture of Rat Hepatocytes</title><author>HIROTA, Kouichi ; KAWANISHI, Toru ; SUNOUCHI, Momoko ; OHNO, Yasuo ; TAKANAKA, Akira ; YAMAZOE, Yasushi ; KATO, Ryuichi ; MURAKOSHI, Yoshie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3380-d3a37a383d1e616da2d56c646c89381c3ad211c625ce9b3133548a97a5734f6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Cytochrome P-450 Enzyme System - biosynthesis</topic><topic>In Vitro Techniques</topic><topic>Isoenzymes - antagonists & inhibitors</topic><topic>Isoenzymes - biosynthesis</topic><topic>Isoenzymes - metabolism</topic><topic>Liver - drug effects</topic><topic>Liver - enzymology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metyrapone - pharmacology</topic><topic>Miscellaneous</topic><topic>Mixed Function Oxygenases - antagonists & inhibitors</topic><topic>Mixed Function Oxygenases - metabolism</topic><topic>Pharmacology. Drug treatments</topic><topic>Phenobarbital - pharmacology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><toplevel>online_resources</toplevel><creatorcontrib>HIROTA, Kouichi</creatorcontrib><creatorcontrib>KAWANISHI, Toru</creatorcontrib><creatorcontrib>SUNOUCHI, Momoko</creatorcontrib><creatorcontrib>OHNO, Yasuo</creatorcontrib><creatorcontrib>TAKANAKA, Akira</creatorcontrib><creatorcontrib>YAMAZOE, Yasushi</creatorcontrib><creatorcontrib>KATO, Ryuichi</creatorcontrib><creatorcontrib>MURAKOSHI, Yoshie</creatorcontrib><creatorcontrib>School of Medicine</creatorcontrib><creatorcontrib>Department of Pharmacology</creatorcontrib><creatorcontrib>National Institute of Hygienic Sciences</creatorcontrib><creatorcontrib>College of Science and Technology</creatorcontrib><creatorcontrib>Department of Pharmacy</creatorcontrib><creatorcontrib>Nihon University</creatorcontrib><creatorcontrib>Keio University</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Japanese Journal of Pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HIROTA, Kouichi</au><au>KAWANISHI, Toru</au><au>SUNOUCHI, Momoko</au><au>OHNO, Yasuo</au><au>TAKANAKA, Akira</au><au>YAMAZOE, Yasushi</au><au>KATO, Ryuichi</au><au>MURAKOSHI, Yoshie</au><aucorp>School of Medicine</aucorp><aucorp>Department of Pharmacology</aucorp><aucorp>National Institute of Hygienic Sciences</aucorp><aucorp>College of Science and Technology</aucorp><aucorp>Department of Pharmacy</aucorp><aucorp>Nihon University</aucorp><aucorp>Keio University</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of Cytochrome P-450c and P-450d by Metyrapone in the Primary Culture of Rat Hepatocytes</atitle><jtitle>Japanese Journal of Pharmacology</jtitle><addtitle>Jpn J Pharmacol</addtitle><date>1989</date><risdate>1989</risdate><volume>51</volume><issue>1</issue><spage>136</spage><epage>139</epage><pages>136-139</pages><issn>0021-5198</issn><eissn>1347-3506</eissn><coden>JJPAAZ</coden><abstract>The effects of metyrapone on qualitative changes in cytochrome P-450-dependent drug metabolizing activities in primary cultures of rat hepatocytes were investigated. Metyrapone apparently increased benzo(a)pyrene hydroxylation and maintained both ethoxycoumarin-O-deethylation and propoxycoumarin-O-depro-pylation. whereas it had little effect on methoxycoumarin-O-demethylation Furthermore, P-450d (high spin type of P-448) as well as P-450c (low spin type of P-448) were induced by metyrapone. while P-450b and P-450e were not. In conclusion, metyrapone act as a 3-methylcholanthrene-like inducer in the primary cultures of rat hepatocytes</abstract><cop>Kyoto</cop><pub>The Japanese Pharmacological Society</pub><pmid>2810935</pmid><doi>10.1016/S0021-5198(19)40148-0</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Japanese Journal of Pharmacology, 1989, Vol.51 (1), p.136-139 |
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| language | eng |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Biological and medical sciences Cells, Cultured Cytochrome P-450 Enzyme System - biosynthesis In Vitro Techniques Isoenzymes - antagonists & inhibitors Isoenzymes - biosynthesis Isoenzymes - metabolism Liver - drug effects Liver - enzymology Male Medical sciences Metyrapone - pharmacology Miscellaneous Mixed Function Oxygenases - antagonists & inhibitors Mixed Function Oxygenases - metabolism Pharmacology. Drug treatments Phenobarbital - pharmacology Rats Rats, Inbred Strains |
| title | Induction of Cytochrome P-450c and P-450d by Metyrapone in the Primary Culture of Rat Hepatocytes |
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