Alternate Junctions and Microheterogeneity of Tlr1, a Developmentally Regulated DNA Rearrangement in Tetrahymena thermophila
A large number of developmentally regulated DNA rearrangements occur during the development of the macronucleus in Tetrahymena thermophila, Tlr1 is a deletion element which has large inverted repeats near the rearrangement junctions and deletes more than 13 kbp of internal DNA. Previous analysis of...
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| Veröffentlicht in: | The Journal of eukaryotic microbiology 1997-09, Vol.44 (5), p.518-522 |
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| container_title | The Journal of eukaryotic microbiology |
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| creator | PATIL, NAMRATA S. HEMPEN, PAULA M. UDANI, RUPA A. KARRER, KATHLEEN M. |
| description | A large number of developmentally regulated DNA rearrangements occur during the development of the macronucleus in Tetrahymena thermophila, Tlr1 is a deletion element which has large inverted repeats near the rearrangement junctions and deletes more than 13 kbp of internal DNA. Previous analysis of caryonidal lines revealed alternate left junctions for the Tlr1 rearrangement in B strain cells. We show here that C2 strain Tetrahymena also use alternate rearrangement junctions. We have mapped and sequenced two additional rearrangement variants and find that both the left and right can vary over a range of approximately 200 bp. We also demonstrate the presence of sequence microheterogeneity in the most commonly found Tlr1 rearrangement product. |
| doi_str_mv | 10.1111/j.1550-7408.1997.tb05733.x |
| format | Article |
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Previous analysis of caryonidal lines revealed alternate left junctions for the Tlr1 rearrangement in B strain cells. We show here that C2 strain Tetrahymena also use alternate rearrangement junctions. We have mapped and sequenced two additional rearrangement variants and find that both the left and right can vary over a range of approximately 200 bp. We also demonstrate the presence of sequence microheterogeneity in the most commonly found Tlr1 rearrangement product.</description><identifier>ISSN: 1066-5234</identifier><identifier>EISSN: 1550-7408</identifier><identifier>DOI: 10.1111/j.1550-7408.1997.tb05733.x</identifier><identifier>PMID: 9304822</identifier><identifier>CODEN: JEMIED</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>A-rule ; Animals ; Biochemistry. Physiology. Immunology. 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Previous analysis of caryonidal lines revealed alternate left junctions for the Tlr1 rearrangement in B strain cells. We show here that C2 strain Tetrahymena also use alternate rearrangement junctions. We have mapped and sequenced two additional rearrangement variants and find that both the left and right can vary over a range of approximately 200 bp. We also demonstrate the presence of sequence microheterogeneity in the most commonly found Tlr1 rearrangement product.</description><subject>A-rule</subject><subject>Animals</subject><subject>Biochemistry. Physiology. Immunology. Molecular biology</subject><subject>Biological and medical sciences</subject><subject>Cell Nucleus</subject><subject>ciliates</subject><subject>DNA, Protozoan - analysis</subject><subject>DNA, Protozoan - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Rearrangement - genetics</subject><subject>Genetic Variation - genetics</subject><subject>inverted repeats</subject><subject>Protozoa</subject><subject>rearrangement variants</subject><subject>Restriction Mapping</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Deletion - genetics</subject><subject>Tetrahymena thermophila - genetics</subject><issn>1066-5234</issn><issn>1550-7408</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkE-P0zAQxSMEWpaFj4BkIcSJBP-J7ZgDUrW7FFalIOhqj5aTTNoUJyl2Ao3Eh8dRo97xZTx6b549vyh6RXBCwnm3TwjnOJYpzhKilEz6HHPJWHJ8FF2epcfhjoWIOWXp0-iZ93uMiaCEXEQXiuE0o_Qy-ruwPbjW9IDuhrbo6671yLQl-lIXrttBELsttFD3I-oqtLGOvEUG3cBvsN2hgbY31o7oO2wHG0JKdLNehM44Z9otTDqqW7SB3pndGFqD-h24pjvsamueR08qYz28mOtVdP_xdnP9KV59XX6-XqziImUii7OSVEblnFdMqIpmhuUqT1NGpSClklzmgnBCKlzR0ggsFZGC8qLkUrACSMGuojen3IPrfg3ge93UvgBrTQvd4LVUVElGcTC-PxnD7t47qPTB1Y1xoyZYT-j1Xk989cRXT-j1jF4fw_DL-ZUhb6A8j86sg_561o0vjK0CoaL2ZxvNOMOZDLYPJ9uf2sL4Hx_Qd7f3nGQhID4F1L6H4znAuJ9aSCa5flgv9Wb58G31I13rNfsH2Byw_Q</recordid><startdate>199709</startdate><enddate>199709</enddate><creator>PATIL, NAMRATA S.</creator><creator>HEMPEN, PAULA M.</creator><creator>UDANI, RUPA A.</creator><creator>KARRER, KATHLEEN M.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199709</creationdate><title>Alternate Junctions and Microheterogeneity of Tlr1, a Developmentally Regulated DNA Rearrangement in Tetrahymena thermophila</title><author>PATIL, NAMRATA S. ; HEMPEN, PAULA M. ; UDANI, RUPA A. ; KARRER, KATHLEEN M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4368-8d1fa9b55f369f28a3b9b4432761d9757b61511f0f2da607917625cd5763ce1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>A-rule</topic><topic>Animals</topic><topic>Biochemistry. Physiology. Immunology. Molecular biology</topic><topic>Biological and medical sciences</topic><topic>Cell Nucleus</topic><topic>ciliates</topic><topic>DNA, Protozoan - analysis</topic><topic>DNA, Protozoan - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Rearrangement - genetics</topic><topic>Genetic Variation - genetics</topic><topic>inverted repeats</topic><topic>Protozoa</topic><topic>rearrangement variants</topic><topic>Restriction Mapping</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Deletion - genetics</topic><topic>Tetrahymena thermophila - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PATIL, NAMRATA S.</creatorcontrib><creatorcontrib>HEMPEN, PAULA M.</creatorcontrib><creatorcontrib>UDANI, RUPA A.</creatorcontrib><creatorcontrib>KARRER, KATHLEEN M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of eukaryotic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PATIL, NAMRATA S.</au><au>HEMPEN, PAULA M.</au><au>UDANI, RUPA A.</au><au>KARRER, KATHLEEN M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alternate Junctions and Microheterogeneity of Tlr1, a Developmentally Regulated DNA Rearrangement in Tetrahymena thermophila</atitle><jtitle>The Journal of eukaryotic microbiology</jtitle><addtitle>J Eukaryot Microbiol</addtitle><date>1997-09</date><risdate>1997</risdate><volume>44</volume><issue>5</issue><spage>518</spage><epage>522</epage><pages>518-522</pages><issn>1066-5234</issn><eissn>1550-7408</eissn><coden>JEMIED</coden><abstract>A large number of developmentally regulated DNA rearrangements occur during the development of the macronucleus in Tetrahymena thermophila, Tlr1 is a deletion element which has large inverted repeats near the rearrangement junctions and deletes more than 13 kbp of internal DNA. Previous analysis of caryonidal lines revealed alternate left junctions for the Tlr1 rearrangement in B strain cells. We show here that C2 strain Tetrahymena also use alternate rearrangement junctions. We have mapped and sequenced two additional rearrangement variants and find that both the left and right can vary over a range of approximately 200 bp. We also demonstrate the presence of sequence microheterogeneity in the most commonly found Tlr1 rearrangement product.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9304822</pmid><doi>10.1111/j.1550-7408.1997.tb05733.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | The Journal of eukaryotic microbiology, 1997-09, Vol.44 (5), p.518-522 |
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| subjects | A-rule Animals Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Cell Nucleus ciliates DNA, Protozoan - analysis DNA, Protozoan - genetics Fundamental and applied biological sciences. Psychology Gene Rearrangement - genetics Genetic Variation - genetics inverted repeats Protozoa rearrangement variants Restriction Mapping Sequence Analysis, DNA Sequence Deletion - genetics Tetrahymena thermophila - genetics |
| title | Alternate Junctions and Microheterogeneity of Tlr1, a Developmentally Regulated DNA Rearrangement in Tetrahymena thermophila |
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