A technique for the culture of Barrett's oesophageal cells
ABSTRACT Establishment of cells in tissue culture from Barrett's columnar epithelium has been difficult. The aim of this study was to develop a successful tissue culture method employing a serumfree medium for cultivation of Barrett's epithelial cells. Fragments of Barrett's mucosal t...
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| Veröffentlicht in: | Journal of gastroenterology and hepatology 1997-08, Vol.12 (8), p.606-611 |
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| container_title | Journal of gastroenterology and hepatology |
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| creator | KHAN, SEEMA M PILLAY, S PRAGA PAPADIMOS, DAVID YONG, JOHN WK ROBERTS, H JOHN V CRAWFORD, DARRELL H |
| description | ABSTRACT
Establishment of cells in tissue culture from Barrett's columnar epithelium has been difficult. The aim of this study was to develop a successful tissue culture method employing a serumfree medium for cultivation of Barrett's epithelial cells. Fragments of Barrett's mucosal tissue were explanted in a 3:1 mixture of Dulbecco's modification of Eagle's medium and Ham's F12, to initiate the outgrowth of epithelial cells. Subsequently, a commercial serum‐free medium (formulated for the growth of keratinocytes) was used for the propagation of Barrett's oesophagus cells without fibroblast growth. Cells established in culture retained their epithelial morphology, stained positive for cytokeratin, and contained Alcian blue (pH 2.5) and periodic acid‐Schiff reagent‐positive/diastase‐resistant vacuoles, confirming their origin from Barrett's epithelium. Electron microscopy showed tonofilaments, microvilli and desmosomes. Coating the surface of culture vessels was not required and four cell strains could be passaged up to 20 times with no fibroblast growth, in the keratinocyte serumfree medium. |
| doi_str_mv | 10.1111/j.1440-1746.1997.tb00493.x |
| format | Article |
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Establishment of cells in tissue culture from Barrett's columnar epithelium has been difficult. The aim of this study was to develop a successful tissue culture method employing a serumfree medium for cultivation of Barrett's epithelial cells. Fragments of Barrett's mucosal tissue were explanted in a 3:1 mixture of Dulbecco's modification of Eagle's medium and Ham's F12, to initiate the outgrowth of epithelial cells. Subsequently, a commercial serum‐free medium (formulated for the growth of keratinocytes) was used for the propagation of Barrett's oesophagus cells without fibroblast growth. Cells established in culture retained their epithelial morphology, stained positive for cytokeratin, and contained Alcian blue (pH 2.5) and periodic acid‐Schiff reagent‐positive/diastase‐resistant vacuoles, confirming their origin from Barrett's epithelium. Electron microscopy showed tonofilaments, microvilli and desmosomes. Coating the surface of culture vessels was not required and four cell strains could be passaged up to 20 times with no fibroblast growth, in the keratinocyte serumfree medium.</description><identifier>ISSN: 0815-9319</identifier><identifier>EISSN: 1440-1746</identifier><identifier>DOI: 10.1111/j.1440-1746.1997.tb00493.x</identifier><identifier>PMID: 9304514</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Barrett Esophagus - pathology ; Biological and medical sciences ; Cell Culture Techniques - methods ; Culture Media ; Epithelium - pathology ; Epithelium - ultrastructure ; Esophagus ; Esophagus - pathology ; Esophagus - ultrastructure ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Medical sciences ; Microscopy, Electron ; Other diseases. Semiology</subject><ispartof>Journal of gastroenterology and hepatology, 1997-08, Vol.12 (8), p.606-611</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3516-7383c5e67081fd05787de81eea21e792fb64960d4177210ab609497f7d61db2e3</citedby><cites>FETCH-LOGICAL-c3516-7383c5e67081fd05787de81eea21e792fb64960d4177210ab609497f7d61db2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1440-1746.1997.tb00493.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1440-1746.1997.tb00493.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>310,311,315,782,786,791,792,1419,23937,23938,25147,27931,27932,45581,45582</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2776815$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9304514$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KHAN, SEEMA M</creatorcontrib><creatorcontrib>PILLAY, S PRAGA</creatorcontrib><creatorcontrib>PAPADIMOS, DAVID</creatorcontrib><creatorcontrib>YONG, JOHN WK</creatorcontrib><creatorcontrib>ROBERTS, H JOHN V</creatorcontrib><creatorcontrib>CRAWFORD, DARRELL H</creatorcontrib><title>A technique for the culture of Barrett's oesophageal cells</title><title>Journal of gastroenterology and hepatology</title><addtitle>J Gastroenterol Hepatol</addtitle><description>ABSTRACT
Establishment of cells in tissue culture from Barrett's columnar epithelium has been difficult. The aim of this study was to develop a successful tissue culture method employing a serumfree medium for cultivation of Barrett's epithelial cells. Fragments of Barrett's mucosal tissue were explanted in a 3:1 mixture of Dulbecco's modification of Eagle's medium and Ham's F12, to initiate the outgrowth of epithelial cells. Subsequently, a commercial serum‐free medium (formulated for the growth of keratinocytes) was used for the propagation of Barrett's oesophagus cells without fibroblast growth. Cells established in culture retained their epithelial morphology, stained positive for cytokeratin, and contained Alcian blue (pH 2.5) and periodic acid‐Schiff reagent‐positive/diastase‐resistant vacuoles, confirming their origin from Barrett's epithelium. Electron microscopy showed tonofilaments, microvilli and desmosomes. Coating the surface of culture vessels was not required and four cell strains could be passaged up to 20 times with no fibroblast growth, in the keratinocyte serumfree medium.</description><subject>Barrett Esophagus - pathology</subject><subject>Biological and medical sciences</subject><subject>Cell Culture Techniques - methods</subject><subject>Culture Media</subject><subject>Epithelium - pathology</subject><subject>Epithelium - ultrastructure</subject><subject>Esophagus</subject><subject>Esophagus - pathology</subject><subject>Esophagus - ultrastructure</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Other diseases. Semiology</subject><issn>0815-9319</issn><issn>1440-1746</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkNFO2zAUhi00xErHIyBFaBpXyezY8al7MQkQtFRo0ybQJm4sJzlZU9Km2Ilo3x5HiXqPb3xxvvP790fIBaMR8-f7KmJC0JCBkBFTCqImpVQoHu2OyOgw-kRGdMKSUHGmPpNT51bUUxSSE3KiOBUJEyMyvQoazJab8rXFoKht0CwxyNqqaS0GdRFcG2uxaS5dUKOrt0vzH00VZFhV7gs5Lkzl8Gy4x-Tp7vbxZh4-_Jrd31w9hBlPmAyBT3iWoARfpshpAhPIccIQTcwQVFykUihJc8EAYkZNKqkSCgrIJcvTGPmYfOtzt7b2LV2j16XrGpgN1q3TPkNJUNKD0x7MbO2cxUJvbbk2dq8Z1Z04vdKdHd3Z0Z04PYjTO798PrzSpmvMD6uDKT__OsyNy0xVWLPJSnfAYgDpZXvsR4-9lRXuP1BAL2ZzSbtPhH1A6RrcHQKMfdESOCT678-Z_v1nzuLFvzv9zN8BgrSXuQ</recordid><startdate>199708</startdate><enddate>199708</enddate><creator>KHAN, SEEMA M</creator><creator>PILLAY, S PRAGA</creator><creator>PAPADIMOS, DAVID</creator><creator>YONG, JOHN WK</creator><creator>ROBERTS, H JOHN V</creator><creator>CRAWFORD, DARRELL H</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199708</creationdate><title>A technique for the culture of Barrett's oesophageal cells</title><author>KHAN, SEEMA M ; PILLAY, S PRAGA ; PAPADIMOS, DAVID ; YONG, JOHN WK ; ROBERTS, H JOHN V ; CRAWFORD, DARRELL H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3516-7383c5e67081fd05787de81eea21e792fb64960d4177210ab609497f7d61db2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Barrett Esophagus - pathology</topic><topic>Biological and medical sciences</topic><topic>Cell Culture Techniques - methods</topic><topic>Culture Media</topic><topic>Epithelium - pathology</topic><topic>Epithelium - ultrastructure</topic><topic>Esophagus</topic><topic>Esophagus - pathology</topic><topic>Esophagus - ultrastructure</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Other diseases. Semiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KHAN, SEEMA M</creatorcontrib><creatorcontrib>PILLAY, S PRAGA</creatorcontrib><creatorcontrib>PAPADIMOS, DAVID</creatorcontrib><creatorcontrib>YONG, JOHN WK</creatorcontrib><creatorcontrib>ROBERTS, H JOHN V</creatorcontrib><creatorcontrib>CRAWFORD, DARRELL H</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of gastroenterology and hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KHAN, SEEMA M</au><au>PILLAY, S PRAGA</au><au>PAPADIMOS, DAVID</au><au>YONG, JOHN WK</au><au>ROBERTS, H JOHN V</au><au>CRAWFORD, DARRELL H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A technique for the culture of Barrett's oesophageal cells</atitle><jtitle>Journal of gastroenterology and hepatology</jtitle><addtitle>J Gastroenterol Hepatol</addtitle><date>1997-08</date><risdate>1997</risdate><volume>12</volume><issue>8</issue><spage>606</spage><epage>611</epage><pages>606-611</pages><issn>0815-9319</issn><eissn>1440-1746</eissn><abstract>ABSTRACT
Establishment of cells in tissue culture from Barrett's columnar epithelium has been difficult. The aim of this study was to develop a successful tissue culture method employing a serumfree medium for cultivation of Barrett's epithelial cells. Fragments of Barrett's mucosal tissue were explanted in a 3:1 mixture of Dulbecco's modification of Eagle's medium and Ham's F12, to initiate the outgrowth of epithelial cells. Subsequently, a commercial serum‐free medium (formulated for the growth of keratinocytes) was used for the propagation of Barrett's oesophagus cells without fibroblast growth. Cells established in culture retained their epithelial morphology, stained positive for cytokeratin, and contained Alcian blue (pH 2.5) and periodic acid‐Schiff reagent‐positive/diastase‐resistant vacuoles, confirming their origin from Barrett's epithelium. Electron microscopy showed tonofilaments, microvilli and desmosomes. Coating the surface of culture vessels was not required and four cell strains could be passaged up to 20 times with no fibroblast growth, in the keratinocyte serumfree medium.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9304514</pmid><doi>10.1111/j.1440-1746.1997.tb00493.x</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Barrett Esophagus - pathology Biological and medical sciences Cell Culture Techniques - methods Culture Media Epithelium - pathology Epithelium - ultrastructure Esophagus Esophagus - pathology Esophagus - ultrastructure Gastroenterology. Liver. Pancreas. Abdomen Humans Medical sciences Microscopy, Electron Other diseases. Semiology |
| title | A technique for the culture of Barrett's oesophageal cells |
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