Autoantibodies to each protein fraction extracted from cerebral endothelial cell membrane in the sera of patients with multiple sclerosis
Damage to the blood-brain barrier (BBB) occurs in multiple sclerosis (MS), probably due to an immunological mechanism. Anti-endothelial cell antibodies may play a pathogenetic role in the BBB damage. Our previous studies led us to search for which protein fraction extracted from cerebral endothelial...
Gespeichert in:
| Veröffentlicht in: | Journal of neuroimmunology 1989-09, Vol.24 (1), p.41-46 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 46 |
|---|---|
| container_issue | 1 |
| container_start_page | 41 |
| container_title | Journal of neuroimmunology |
| container_volume | 24 |
| creator | Tsukada, Naoyuki Tanaka, Yukio Miyagi, Kouichi Yanagisawa, Nobuo Okano, Akira |
| description | Damage to the blood-brain barrier (BBB) occurs in multiple sclerosis (MS), probably due to an immunological mechanism. Anti-endothelial cell antibodies may play a pathogenetic role in the BBB damage. Our previous studies led us to search for which protein fraction extracted from cerebral endothelial cell membrane was reactive to antibodies in the sera of patients with MS. The antibodies to each protein fraction extracted from the rat cerebral endothelial cell membrane were studied in patients with MS, other neurological diseases and controls using an enzyme-linked immunosorbent assay (ELISA) method. The patients with active relapsing MS (
P < 0.01) displayed significantly higher levels of immunoglobulin G (IgG) binding to the endothelial cell membrane fraction than did the controls. The sera of the same patients (
P < 0.001) also showed significantly higher levels of antibodies to fraction I (8.0 kDa) than did the normal controls. The high levels of IgG binding to fraction II (11.0 kDa) and III (12.3 kDa) were significantly increased in the sera of patients with active relapsing MS compared to normal controls (
P < 0.01). The immune response to the protein fraction extracted from the cerebral endothelial cell membrane fraction may indicate a result of the BBB damage in the case of MS. |
| doi_str_mv | 10.1016/0165-5728(89)90096-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79295491</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0165572889900969</els_id><sourcerecordid>15419046</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-301b349579655ae0cafd9beb744ffdf1091c8c40e0f6455fd9f178eaa71d185e3</originalsourceid><addsrcrecordid>eNqFkc-OFCEQxonRrLOrb6AJB2PWQ6_QAw1cNtls1DXZxMt6JjQUGQzdjED75xF8a2lnMkf3QKjU96tKVX0IvaLkihI6vG-Pd1z08lKqd4oQNXTqCdpQKfpOsp4-RZsT8hydl_KNEMq3TJ2hs54JyYXYoD83S01mrmFMLkDBNWEwdof3OVUIM_bZ2BrSjOFXXUNwLZUmbCHDmE3EMLtUdxBDiy3EiCeYmjADbtVNwAWywcnjvakB5lrwz1B3eFpiDfvYZBshpxLKC_TMm1jg5fG_QF8_fni4vevuv3z6fHtz31lGRe22hI5tBy7UwLkBYo13aoRRMOa985QoaqVlBIgfGOdN9FRIMEZQRyWH7QV6e-jbVvy-QKl6CmWdvM2clqKF6hVnij4KUs6oImxoIDuAti1SMni9z2Ey-bemRK9W6dUHvfqgpdL_rNKqlb0-9l_GCdyp6OhN098cdVOsic2J2YZywgYhKWMrdn3AoB3tR4Csi22HtuBCBlu1S-H_c_wFr_Oy3w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15419046</pqid></control><display><type>article</type><title>Autoantibodies to each protein fraction extracted from cerebral endothelial cell membrane in the sera of patients with multiple sclerosis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Tsukada, Naoyuki ; Tanaka, Yukio ; Miyagi, Kouichi ; Yanagisawa, Nobuo ; Okano, Akira</creator><creatorcontrib>Tsukada, Naoyuki ; Tanaka, Yukio ; Miyagi, Kouichi ; Yanagisawa, Nobuo ; Okano, Akira</creatorcontrib><description>Damage to the blood-brain barrier (BBB) occurs in multiple sclerosis (MS), probably due to an immunological mechanism. Anti-endothelial cell antibodies may play a pathogenetic role in the BBB damage. Our previous studies led us to search for which protein fraction extracted from cerebral endothelial cell membrane was reactive to antibodies in the sera of patients with MS. The antibodies to each protein fraction extracted from the rat cerebral endothelial cell membrane were studied in patients with MS, other neurological diseases and controls using an enzyme-linked immunosorbent assay (ELISA) method. The patients with active relapsing MS (
P < 0.01) displayed significantly higher levels of immunoglobulin G (IgG) binding to the endothelial cell membrane fraction than did the controls. The sera of the same patients (
P < 0.001) also showed significantly higher levels of antibodies to fraction I (8.0 kDa) than did the normal controls. The high levels of IgG binding to fraction II (11.0 kDa) and III (12.3 kDa) were significantly increased in the sera of patients with active relapsing MS compared to normal controls (
P < 0.01). The immune response to the protein fraction extracted from the cerebral endothelial cell membrane fraction may indicate a result of the BBB damage in the case of MS.</description><identifier>ISSN: 0165-5728</identifier><identifier>EISSN: 1872-8421</identifier><identifier>DOI: 10.1016/0165-5728(89)90096-9</identifier><identifier>PMID: 2478577</identifier><identifier>CODEN: JNRIDW</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Adolescent ; Adult ; Animals ; Apoproteins - analysis ; Apoproteins - immunology ; Autoantibodies - analysis ; Autoantibodies - blood ; Autoantibody ; Biological and medical sciences ; Cell Membrane - analysis ; Cell Membrane - immunology ; Cerebral Cortex - analysis ; Cerebral Cortex - immunology ; Electrophoresis, Polyacrylamide Gel ; Endothelial cell membrane, cerebral ; Endothelium - analysis ; Endothelium - immunology ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Male ; Medical sciences ; Middle Aged ; Multiple sclerosis ; Multiple Sclerosis - blood ; Multiple Sclerosis - immunology ; Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis ; Myelin Basic Protein - analysis ; Myelin Basic Protein - immunology ; Myelin Proteins - analysis ; Myelin Proteins - immunology ; Myelin Proteolipid Protein ; Neurology ; Protein fraction ; Rats ; Rats, Inbred Strains</subject><ispartof>Journal of neuroimmunology, 1989-09, Vol.24 (1), p.41-46</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-301b349579655ae0cafd9beb744ffdf1091c8c40e0f6455fd9f178eaa71d185e3</citedby><cites>FETCH-LOGICAL-c417t-301b349579655ae0cafd9beb744ffdf1091c8c40e0f6455fd9f178eaa71d185e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0165-5728(89)90096-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6781447$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2478577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsukada, Naoyuki</creatorcontrib><creatorcontrib>Tanaka, Yukio</creatorcontrib><creatorcontrib>Miyagi, Kouichi</creatorcontrib><creatorcontrib>Yanagisawa, Nobuo</creatorcontrib><creatorcontrib>Okano, Akira</creatorcontrib><title>Autoantibodies to each protein fraction extracted from cerebral endothelial cell membrane in the sera of patients with multiple sclerosis</title><title>Journal of neuroimmunology</title><addtitle>J Neuroimmunol</addtitle><description>Damage to the blood-brain barrier (BBB) occurs in multiple sclerosis (MS), probably due to an immunological mechanism. Anti-endothelial cell antibodies may play a pathogenetic role in the BBB damage. Our previous studies led us to search for which protein fraction extracted from cerebral endothelial cell membrane was reactive to antibodies in the sera of patients with MS. The antibodies to each protein fraction extracted from the rat cerebral endothelial cell membrane were studied in patients with MS, other neurological diseases and controls using an enzyme-linked immunosorbent assay (ELISA) method. The patients with active relapsing MS (
P < 0.01) displayed significantly higher levels of immunoglobulin G (IgG) binding to the endothelial cell membrane fraction than did the controls. The sera of the same patients (
P < 0.001) also showed significantly higher levels of antibodies to fraction I (8.0 kDa) than did the normal controls. The high levels of IgG binding to fraction II (11.0 kDa) and III (12.3 kDa) were significantly increased in the sera of patients with active relapsing MS compared to normal controls (
P < 0.01). The immune response to the protein fraction extracted from the cerebral endothelial cell membrane fraction may indicate a result of the BBB damage in the case of MS.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Animals</subject><subject>Apoproteins - analysis</subject><subject>Apoproteins - immunology</subject><subject>Autoantibodies - analysis</subject><subject>Autoantibodies - blood</subject><subject>Autoantibody</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane - analysis</subject><subject>Cell Membrane - immunology</subject><subject>Cerebral Cortex - analysis</subject><subject>Cerebral Cortex - immunology</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Endothelial cell membrane, cerebral</subject><subject>Endothelium - analysis</subject><subject>Endothelium - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Multiple sclerosis</subject><subject>Multiple Sclerosis - blood</subject><subject>Multiple Sclerosis - immunology</subject><subject>Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis</subject><subject>Myelin Basic Protein - analysis</subject><subject>Myelin Basic Protein - immunology</subject><subject>Myelin Proteins - analysis</subject><subject>Myelin Proteins - immunology</subject><subject>Myelin Proteolipid Protein</subject><subject>Neurology</subject><subject>Protein fraction</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-OFCEQxonRrLOrb6AJB2PWQ6_QAw1cNtls1DXZxMt6JjQUGQzdjED75xF8a2lnMkf3QKjU96tKVX0IvaLkihI6vG-Pd1z08lKqd4oQNXTqCdpQKfpOsp4-RZsT8hydl_KNEMq3TJ2hs54JyYXYoD83S01mrmFMLkDBNWEwdof3OVUIM_bZ2BrSjOFXXUNwLZUmbCHDmE3EMLtUdxBDiy3EiCeYmjADbtVNwAWywcnjvakB5lrwz1B3eFpiDfvYZBshpxLKC_TMm1jg5fG_QF8_fni4vevuv3z6fHtz31lGRe22hI5tBy7UwLkBYo13aoRRMOa985QoaqVlBIgfGOdN9FRIMEZQRyWH7QV6e-jbVvy-QKl6CmWdvM2clqKF6hVnij4KUs6oImxoIDuAti1SMni9z2Ey-bemRK9W6dUHvfqgpdL_rNKqlb0-9l_GCdyp6OhN098cdVOsic2J2YZywgYhKWMrdn3AoB3tR4Csi22HtuBCBlu1S-H_c_wFr_Oy3w</recordid><startdate>19890901</startdate><enddate>19890901</enddate><creator>Tsukada, Naoyuki</creator><creator>Tanaka, Yukio</creator><creator>Miyagi, Kouichi</creator><creator>Yanagisawa, Nobuo</creator><creator>Okano, Akira</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19890901</creationdate><title>Autoantibodies to each protein fraction extracted from cerebral endothelial cell membrane in the sera of patients with multiple sclerosis</title><author>Tsukada, Naoyuki ; Tanaka, Yukio ; Miyagi, Kouichi ; Yanagisawa, Nobuo ; Okano, Akira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-301b349579655ae0cafd9beb744ffdf1091c8c40e0f6455fd9f178eaa71d185e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Animals</topic><topic>Apoproteins - analysis</topic><topic>Apoproteins - immunology</topic><topic>Autoantibodies - analysis</topic><topic>Autoantibodies - blood</topic><topic>Autoantibody</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane - analysis</topic><topic>Cell Membrane - immunology</topic><topic>Cerebral Cortex - analysis</topic><topic>Cerebral Cortex - immunology</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Endothelial cell membrane, cerebral</topic><topic>Endothelium - analysis</topic><topic>Endothelium - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Multiple sclerosis</topic><topic>Multiple Sclerosis - blood</topic><topic>Multiple Sclerosis - immunology</topic><topic>Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis</topic><topic>Myelin Basic Protein - analysis</topic><topic>Myelin Basic Protein - immunology</topic><topic>Myelin Proteins - analysis</topic><topic>Myelin Proteins - immunology</topic><topic>Myelin Proteolipid Protein</topic><topic>Neurology</topic><topic>Protein fraction</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsukada, Naoyuki</creatorcontrib><creatorcontrib>Tanaka, Yukio</creatorcontrib><creatorcontrib>Miyagi, Kouichi</creatorcontrib><creatorcontrib>Yanagisawa, Nobuo</creatorcontrib><creatorcontrib>Okano, Akira</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsukada, Naoyuki</au><au>Tanaka, Yukio</au><au>Miyagi, Kouichi</au><au>Yanagisawa, Nobuo</au><au>Okano, Akira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Autoantibodies to each protein fraction extracted from cerebral endothelial cell membrane in the sera of patients with multiple sclerosis</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>1989-09-01</date><risdate>1989</risdate><volume>24</volume><issue>1</issue><spage>41</spage><epage>46</epage><pages>41-46</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><coden>JNRIDW</coden><abstract>Damage to the blood-brain barrier (BBB) occurs in multiple sclerosis (MS), probably due to an immunological mechanism. Anti-endothelial cell antibodies may play a pathogenetic role in the BBB damage. Our previous studies led us to search for which protein fraction extracted from cerebral endothelial cell membrane was reactive to antibodies in the sera of patients with MS. The antibodies to each protein fraction extracted from the rat cerebral endothelial cell membrane were studied in patients with MS, other neurological diseases and controls using an enzyme-linked immunosorbent assay (ELISA) method. The patients with active relapsing MS (
P < 0.01) displayed significantly higher levels of immunoglobulin G (IgG) binding to the endothelial cell membrane fraction than did the controls. The sera of the same patients (
P < 0.001) also showed significantly higher levels of antibodies to fraction I (8.0 kDa) than did the normal controls. The high levels of IgG binding to fraction II (11.0 kDa) and III (12.3 kDa) were significantly increased in the sera of patients with active relapsing MS compared to normal controls (
P < 0.01). The immune response to the protein fraction extracted from the cerebral endothelial cell membrane fraction may indicate a result of the BBB damage in the case of MS.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2478577</pmid><doi>10.1016/0165-5728(89)90096-9</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-5728 |
| ispartof | Journal of neuroimmunology, 1989-09, Vol.24 (1), p.41-46 |
| issn | 0165-5728 1872-8421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79295491 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adolescent Adult Animals Apoproteins - analysis Apoproteins - immunology Autoantibodies - analysis Autoantibodies - blood Autoantibody Biological and medical sciences Cell Membrane - analysis Cell Membrane - immunology Cerebral Cortex - analysis Cerebral Cortex - immunology Electrophoresis, Polyacrylamide Gel Endothelial cell membrane, cerebral Endothelium - analysis Endothelium - immunology Enzyme-Linked Immunosorbent Assay Female Humans Male Medical sciences Middle Aged Multiple sclerosis Multiple Sclerosis - blood Multiple Sclerosis - immunology Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis Myelin Basic Protein - analysis Myelin Basic Protein - immunology Myelin Proteins - analysis Myelin Proteins - immunology Myelin Proteolipid Protein Neurology Protein fraction Rats Rats, Inbred Strains |
| title | Autoantibodies to each protein fraction extracted from cerebral endothelial cell membrane in the sera of patients with multiple sclerosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T13%3A28%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Autoantibodies%20to%20each%20protein%20fraction%20extracted%20from%20cerebral%20endothelial%20cell%20membrane%20in%20the%20sera%20of%20patients%20with%20multiple%20sclerosis&rft.jtitle=Journal%20of%20neuroimmunology&rft.au=Tsukada,%20Naoyuki&rft.date=1989-09-01&rft.volume=24&rft.issue=1&rft.spage=41&rft.epage=46&rft.pages=41-46&rft.issn=0165-5728&rft.eissn=1872-8421&rft.coden=JNRIDW&rft_id=info:doi/10.1016/0165-5728(89)90096-9&rft_dat=%3Cproquest_cross%3E15419046%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15419046&rft_id=info:pmid/2478577&rft_els_id=0165572889900969&rfr_iscdi=true |