Actions of 3 alpha,5 alpha-tetrahydrodeoxycorticosterone on single neurones of the mesencephalic reticular formation in the rat
The effects of microinjections of 3 alpha,5 alpha-tetrahydrodeoxycorticosterone (3 alpha-THDOC) on single neurones of the mesencephalic reticular formation (MRF) were investigated in rats anaesthetized with urethan. Microinjections of approximately 100 nl of 0.5-2 microM 3 alpha-THDOC inhibited firi...
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| Veröffentlicht in: | Neuroscience letters 1989-09, Vol.104 (1-2), p.115-120 |
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| creator | Ermirio, R Blanchi, D Ruggeri, P Cogo, C E Molinari, C |
| description | The effects of microinjections of 3 alpha,5 alpha-tetrahydrodeoxycorticosterone (3 alpha-THDOC) on single neurones of the mesencephalic reticular formation (MRF) were investigated in rats anaesthetized with urethan. Microinjections of approximately 100 nl of 0.5-2 microM 3 alpha-THDOC inhibited firing of 105 of 112 neurones (94%). Microinjections of approximately 100 nl of 100-250 nM of 3 alpha-THDOC did not alter neuronal activity, but in 52 of 68 cases (76%) it potentiated the inhibitory action of microiontophoretically applied gamma-aminobutyric acid (GABA). The 3 beta-isomer of tetrahydrodeoxycorticosterone did not elicit any changes in neuronal firing. The effects of 3 alpha-THDOC were reversibly antagonized by microiontophoretically applied bicuculline. This 'in vivo' study supports the hypothesis that 3 alpha-THDOC may function as endogenous modulator of GABAA-mediated inhibition in various physiopathological conditions. |
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Microinjections of approximately 100 nl of 0.5-2 microM 3 alpha-THDOC inhibited firing of 105 of 112 neurones (94%). Microinjections of approximately 100 nl of 100-250 nM of 3 alpha-THDOC did not alter neuronal activity, but in 52 of 68 cases (76%) it potentiated the inhibitory action of microiontophoretically applied gamma-aminobutyric acid (GABA). The 3 beta-isomer of tetrahydrodeoxycorticosterone did not elicit any changes in neuronal firing. The effects of 3 alpha-THDOC were reversibly antagonized by microiontophoretically applied bicuculline. This 'in vivo' study supports the hypothesis that 3 alpha-THDOC may function as endogenous modulator of GABAA-mediated inhibition in various physiopathological conditions.</description><identifier>ISSN: 0304-3940</identifier><identifier>PMID: 2812524</identifier><language>eng</language><publisher>Ireland</publisher><subject>Action Potentials - drug effects ; Animals ; Bicuculline - pharmacology ; Corticosterone - analogs & derivatives ; Corticosterone - antagonists & inhibitors ; Corticosterone - pharmacology ; gamma-Aminobutyric Acid - pharmacology ; Iontophoresis ; Male ; Neurons - drug effects ; Neurons - physiology ; Rats ; Rats, Inbred Strains ; Reticular Formation - cytology ; Reticular Formation - drug effects ; Reticular Formation - physiology</subject><ispartof>Neuroscience letters, 1989-09, Vol.104 (1-2), p.115-120</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2812524$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ermirio, R</creatorcontrib><creatorcontrib>Blanchi, D</creatorcontrib><creatorcontrib>Ruggeri, P</creatorcontrib><creatorcontrib>Cogo, C E</creatorcontrib><creatorcontrib>Molinari, C</creatorcontrib><title>Actions of 3 alpha,5 alpha-tetrahydrodeoxycorticosterone on single neurones of the mesencephalic reticular formation in the rat</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>The effects of microinjections of 3 alpha,5 alpha-tetrahydrodeoxycorticosterone (3 alpha-THDOC) on single neurones of the mesencephalic reticular formation (MRF) were investigated in rats anaesthetized with urethan. Microinjections of approximately 100 nl of 0.5-2 microM 3 alpha-THDOC inhibited firing of 105 of 112 neurones (94%). Microinjections of approximately 100 nl of 100-250 nM of 3 alpha-THDOC did not alter neuronal activity, but in 52 of 68 cases (76%) it potentiated the inhibitory action of microiontophoretically applied gamma-aminobutyric acid (GABA). The 3 beta-isomer of tetrahydrodeoxycorticosterone did not elicit any changes in neuronal firing. The effects of 3 alpha-THDOC were reversibly antagonized by microiontophoretically applied bicuculline. This 'in vivo' study supports the hypothesis that 3 alpha-THDOC may function as endogenous modulator of GABAA-mediated inhibition in various physiopathological conditions.</description><subject>Action Potentials - drug effects</subject><subject>Animals</subject><subject>Bicuculline - pharmacology</subject><subject>Corticosterone - analogs & derivatives</subject><subject>Corticosterone - antagonists & inhibitors</subject><subject>Corticosterone - pharmacology</subject><subject>gamma-Aminobutyric Acid - pharmacology</subject><subject>Iontophoresis</subject><subject>Male</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Reticular Formation - cytology</subject><subject>Reticular Formation - drug effects</subject><subject>Reticular Formation - physiology</subject><issn>0304-3940</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkMtqwzAQRbVoSdO0n1DQqqsaxpYUy8sQ-ggEusneyNK4UZElV5KhWfXXm9fqwnDmXLg3ZA4MeMEaDnfkPqVvABCl4DMyq2RZiYrPyd9KZxt8oqGnjCo37tWLuGSRMUe1P5gYDIbfgw4xWx1Sxhg80uBpsv7LIfU4nS5nR94jHTCh13hUOKtpxOPX5FSkfYiDOrVR689gVPmB3PbKJXy85oLs3l53649i-_m-Wa-2xSgYL2ouGe9kyftONQqhMlAb0ZmukdrISvflkjW6lFpIBmZZC9n3APUSsNQCULEFeb5oxxh-Jky5HWzS6JzyGKbU1k3VsAbgCD5dwakb0LRjtIOKh_Y6GPsHooxorg</recordid><startdate>19890925</startdate><enddate>19890925</enddate><creator>Ermirio, R</creator><creator>Blanchi, D</creator><creator>Ruggeri, P</creator><creator>Cogo, C E</creator><creator>Molinari, C</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19890925</creationdate><title>Actions of 3 alpha,5 alpha-tetrahydrodeoxycorticosterone on single neurones of the mesencephalic reticular formation in the rat</title><author>Ermirio, R ; Blanchi, D ; Ruggeri, P ; Cogo, C E ; Molinari, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p534-74834b814fba9ae02d07d5bdb98cd82cf1639c18c5830d6758ff00760e1c50ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Action Potentials - drug effects</topic><topic>Animals</topic><topic>Bicuculline - pharmacology</topic><topic>Corticosterone - analogs & derivatives</topic><topic>Corticosterone - antagonists & inhibitors</topic><topic>Corticosterone - pharmacology</topic><topic>gamma-Aminobutyric Acid - pharmacology</topic><topic>Iontophoresis</topic><topic>Male</topic><topic>Neurons - drug effects</topic><topic>Neurons - physiology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Reticular Formation - cytology</topic><topic>Reticular Formation - drug effects</topic><topic>Reticular Formation - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ermirio, R</creatorcontrib><creatorcontrib>Blanchi, D</creatorcontrib><creatorcontrib>Ruggeri, P</creatorcontrib><creatorcontrib>Cogo, C E</creatorcontrib><creatorcontrib>Molinari, C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ermirio, R</au><au>Blanchi, D</au><au>Ruggeri, P</au><au>Cogo, C E</au><au>Molinari, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Actions of 3 alpha,5 alpha-tetrahydrodeoxycorticosterone on single neurones of the mesencephalic reticular formation in the rat</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1989-09-25</date><risdate>1989</risdate><volume>104</volume><issue>1-2</issue><spage>115</spage><epage>120</epage><pages>115-120</pages><issn>0304-3940</issn><abstract>The effects of microinjections of 3 alpha,5 alpha-tetrahydrodeoxycorticosterone (3 alpha-THDOC) on single neurones of the mesencephalic reticular formation (MRF) were investigated in rats anaesthetized with urethan. Microinjections of approximately 100 nl of 0.5-2 microM 3 alpha-THDOC inhibited firing of 105 of 112 neurones (94%). Microinjections of approximately 100 nl of 100-250 nM of 3 alpha-THDOC did not alter neuronal activity, but in 52 of 68 cases (76%) it potentiated the inhibitory action of microiontophoretically applied gamma-aminobutyric acid (GABA). The 3 beta-isomer of tetrahydrodeoxycorticosterone did not elicit any changes in neuronal firing. The effects of 3 alpha-THDOC were reversibly antagonized by microiontophoretically applied bicuculline. This 'in vivo' study supports the hypothesis that 3 alpha-THDOC may function as endogenous modulator of GABAA-mediated inhibition in various physiopathological conditions.</abstract><cop>Ireland</cop><pmid>2812524</pmid><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0304-3940 |
| ispartof | Neuroscience letters, 1989-09, Vol.104 (1-2), p.115-120 |
| issn | 0304-3940 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Action Potentials - drug effects Animals Bicuculline - pharmacology Corticosterone - analogs & derivatives Corticosterone - antagonists & inhibitors Corticosterone - pharmacology gamma-Aminobutyric Acid - pharmacology Iontophoresis Male Neurons - drug effects Neurons - physiology Rats Rats, Inbred Strains Reticular Formation - cytology Reticular Formation - drug effects Reticular Formation - physiology |
| title | Actions of 3 alpha,5 alpha-tetrahydrodeoxycorticosterone on single neurones of the mesencephalic reticular formation in the rat |
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