Repression of the λ pcin promoter by integrative host factor
The cin-1 mutation creates a new promoter (pcin) in the tR1 region of bacteriophage lambda. The pcin promoter transcribes the cI repressor gene constitutively. λ cin-1 does not propagate on Escherichia coli mutants lacking the integrative host factor (IHF). λ cI − cin-1 grows normally in IHF − mutan...
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| Veröffentlicht in: | Journal of molecular biology 1989-09, Vol.209 (1), p.55-64 |
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| container_title | Journal of molecular biology |
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| creator | Griffo, G. Oppenheim, A.B. Gottesman, M.E. |
| description | The
cin-1 mutation creates a new promoter (pcin) in the tR1 region of bacteriophage lambda. The pcin promoter transcribes the
cI repressor gene constitutively. λ
cin-1 does not propagate on
Escherichia coli mutants lacking the integrative host factor (IHF). λ
cI
−
cin-1 grows normally in IHF
− mutants, indicating that repressor overproduction from pcin blocks lytic growth. The presence of an IHF binding site which overlaps the pcin promoter led us to the hypothesis that IHF functions as a repressor of pcin transcription. We find that the pcin promoter is fivefold more active in a host lacking IHF than in wild-type cells.
In vitro studies show that IHF directly inhibits transcription initiation at pcin. Abortive initiation and gel retardation assays demonstrate that IHF interferes with the binding of RNA polymerase to the pcin promoter. RNA polymerase bound in an open promoter complex is resistant to IHF. We propose that IHF binding to the pcin promoter region blocks the binding of RNA polymerase to the promoter, either by covering specific nucleotides or by distorting DNA structure. |
| doi_str_mv | 10.1016/0022-2836(89)90169-1 |
| format | Article |
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cin-1 mutation creates a new promoter (pcin) in the tR1 region of bacteriophage lambda. The pcin promoter transcribes the
cI repressor gene constitutively. λ
cin-1 does not propagate on
Escherichia coli mutants lacking the integrative host factor (IHF). λ
cI
−
cin-1 grows normally in IHF
− mutants, indicating that repressor overproduction from pcin blocks lytic growth. The presence of an IHF binding site which overlaps the pcin promoter led us to the hypothesis that IHF functions as a repressor of pcin transcription. We find that the pcin promoter is fivefold more active in a host lacking IHF than in wild-type cells.
In vitro studies show that IHF directly inhibits transcription initiation at pcin. Abortive initiation and gel retardation assays demonstrate that IHF interferes with the binding of RNA polymerase to the pcin promoter. RNA polymerase bound in an open promoter complex is resistant to IHF. We propose that IHF binding to the pcin promoter region blocks the binding of RNA polymerase to the promoter, either by covering specific nucleotides or by distorting DNA structure.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1016/0022-2836(89)90169-1</identifier><identifier>PMID: 2530356</identifier><identifier>CODEN: JMOBAK</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Bacterial Proteins - genetics ; Bacteriophage lambda ; Base Sequence ; Binding Sites ; Biological and medical sciences ; DNA-Binding Proteins - genetics ; DNA-Directed RNA Polymerases - metabolism ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Genes. Genome ; Integration Host Factors ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutation ; phage lambda ; Promoter Regions, Genetic ; Repressor Proteins - genetics ; Transcription Factors - genetics ; Transcription, Genetic</subject><ispartof>Journal of molecular biology, 1989-09, Vol.209 (1), p.55-64</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-ea63f0ea1476224153fefcf8738811e6d43bc649fec765bbea39890fffd772003</citedby><cites>FETCH-LOGICAL-c463t-ea63f0ea1476224153fefcf8738811e6d43bc649fec765bbea39890fffd772003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-2836(89)90169-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6772719$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2530356$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Griffo, G.</creatorcontrib><creatorcontrib>Oppenheim, A.B.</creatorcontrib><creatorcontrib>Gottesman, M.E.</creatorcontrib><title>Repression of the λ pcin promoter by integrative host factor</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>The
cin-1 mutation creates a new promoter (pcin) in the tR1 region of bacteriophage lambda. The pcin promoter transcribes the
cI repressor gene constitutively. λ
cin-1 does not propagate on
Escherichia coli mutants lacking the integrative host factor (IHF). λ
cI
−
cin-1 grows normally in IHF
− mutants, indicating that repressor overproduction from pcin blocks lytic growth. The presence of an IHF binding site which overlaps the pcin promoter led us to the hypothesis that IHF functions as a repressor of pcin transcription. We find that the pcin promoter is fivefold more active in a host lacking IHF than in wild-type cells.
In vitro studies show that IHF directly inhibits transcription initiation at pcin. Abortive initiation and gel retardation assays demonstrate that IHF interferes with the binding of RNA polymerase to the pcin promoter. RNA polymerase bound in an open promoter complex is resistant to IHF. We propose that IHF binding to the pcin promoter region blocks the binding of RNA polymerase to the promoter, either by covering specific nucleotides or by distorting DNA structure.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacteriophage lambda</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes. Genome</subject><subject>Integration Host Factors</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>phage lambda</subject><subject>Promoter Regions, Genetic</subject><subject>Repressor Proteins - genetics</subject><subject>Transcription Factors - genetics</subject><subject>Transcription, Genetic</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkNtKAzEQhoMotR7eQCEXInqxmsNuNrlQkOIJBEH0OmSzE420m5qkQp_Nd_CZ3NrSS70amPnmZ-ZD6ICSM0qoOCeEsYJJLk6kOlV9RxV0Aw0pkaqQgstNNFwj22gnpXdCSMVLOUADVnHCKzFEF08wjZCSDx0ODuc3wN9feGp9h6cxTEKGiJs59l2G12iy_wT8FlLGztgc4h7acmacYH9Vd9HLzfXz6K54eLy9H109FLYUPBdgBHcEDC1rwVhJK-7AWSdrLiWlINqSN1aUyoGtRdU0YLiSijjn2rpmhPBddLzM7W_6mEHKeuKThfHYdBBmSdeKKV73uf-BtCo5I3KRWC5BG0NKEZyeRj8xca4p0Qu9euFOL9xpqfSvXk37tcNV_qyZQLteWvns50eruUnWjF00nfVpjYn-nZqqHrtcYtBL-_QQdbIeOgutj2CzboP_-44f25aV4Q</recordid><startdate>19890905</startdate><enddate>19890905</enddate><creator>Griffo, G.</creator><creator>Oppenheim, A.B.</creator><creator>Gottesman, M.E.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19890905</creationdate><title>Repression of the λ pcin promoter by integrative host factor</title><author>Griffo, G. ; Oppenheim, A.B. ; Gottesman, M.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-ea63f0ea1476224153fefcf8738811e6d43bc649fec765bbea39890fffd772003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacteriophage lambda</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Directed RNA Polymerases - metabolism</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes. Genome</topic><topic>Integration Host Factors</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>phage lambda</topic><topic>Promoter Regions, Genetic</topic><topic>Repressor Proteins - genetics</topic><topic>Transcription Factors - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Griffo, G.</creatorcontrib><creatorcontrib>Oppenheim, A.B.</creatorcontrib><creatorcontrib>Gottesman, M.E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Griffo, G.</au><au>Oppenheim, A.B.</au><au>Gottesman, M.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Repression of the λ pcin promoter by integrative host factor</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1989-09-05</date><risdate>1989</risdate><volume>209</volume><issue>1</issue><spage>55</spage><epage>64</epage><pages>55-64</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><coden>JMOBAK</coden><abstract>The
cin-1 mutation creates a new promoter (pcin) in the tR1 region of bacteriophage lambda. The pcin promoter transcribes the
cI repressor gene constitutively. λ
cin-1 does not propagate on
Escherichia coli mutants lacking the integrative host factor (IHF). λ
cI
−
cin-1 grows normally in IHF
− mutants, indicating that repressor overproduction from pcin blocks lytic growth. The presence of an IHF binding site which overlaps the pcin promoter led us to the hypothesis that IHF functions as a repressor of pcin transcription. We find that the pcin promoter is fivefold more active in a host lacking IHF than in wild-type cells.
In vitro studies show that IHF directly inhibits transcription initiation at pcin. Abortive initiation and gel retardation assays demonstrate that IHF interferes with the binding of RNA polymerase to the pcin promoter. RNA polymerase bound in an open promoter complex is resistant to IHF. We propose that IHF binding to the pcin promoter region blocks the binding of RNA polymerase to the promoter, either by covering specific nucleotides or by distorting DNA structure.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>2530356</pmid><doi>10.1016/0022-2836(89)90169-1</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of molecular biology, 1989-09, Vol.209 (1), p.55-64 |
| issn | 0022-2836 1089-8638 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Bacterial Proteins - genetics Bacteriophage lambda Base Sequence Binding Sites Biological and medical sciences DNA-Binding Proteins - genetics DNA-Directed RNA Polymerases - metabolism Escherichia coli Fundamental and applied biological sciences. Psychology Genes. Genome Integration Host Factors Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutation phage lambda Promoter Regions, Genetic Repressor Proteins - genetics Transcription Factors - genetics Transcription, Genetic |
| title | Repression of the λ pcin promoter by integrative host factor |
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