Activation signals in human lymphocytes. Incorporation of polyunsaturated fatty acids into plasma membrane phospholipids regulates IL-2 synthesis via sustained activation of protein kinase C
Human PBL activated with anti-TCR/CD3 mAb express high affinity receptors for IL-2, synthesize IL-2, and subsequently proliferate. In contrast, lymphocytes activated by dioctanoylglycerol (DiC8) and ionomycin express high affinity receptors; however, no IL-2 synthesis is detectable. Anti-TCR/CD3 ant...
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Veröffentlicht in: | The Journal of immunology (1950) 1989-11, Vol.143 (9), p.2806-2813 |
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description | Human PBL activated with anti-TCR/CD3 mAb express high affinity receptors for IL-2, synthesize IL-2, and subsequently proliferate. In contrast, lymphocytes activated by dioctanoylglycerol (DiC8) and ionomycin express high affinity receptors; however, no IL-2 synthesis is detectable. Anti-TCR/CD3 antibodies, as well as DiC8 cause translocation of protein kinase C (PKC) from the cytosol to the plasma membrane. In DiC8-stimulated cells translocation of PKC is detectable after 15 min, then it declines to control levels. In lymphocytes activated by antiTCR/CD3 mAb translocation of PKC is detectable after 15 min, then it declines to control levels, followed by a second, long lasting activation of the enzyme up to 4 h. Addition of polyunsaturated fatty acids to DiC8 + ionomycin-treated cells leads to IL-2 synthesis and proliferation. Incorporation of poly-unsaturated fatty acids into plasma membrane phospholipids results in long term activation of PKC. The results suggest that elevated incorporation of polyunsaturated fatty acids and thus continuous activation and translocation of PKC represents a necessary early signal for IL-2 synthesis and proliferation in human lymphocytes. |
doi_str_mv | 10.4049/jimmunol.143.9.2806 |
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Incorporation of polyunsaturated fatty acids into plasma membrane phospholipids regulates IL-2 synthesis via sustained activation of protein kinase C</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Szamel, M ; Rehermann, B ; Krebs, B ; Kurrle, R ; Resch, K</creator><creatorcontrib>Szamel, M ; Rehermann, B ; Krebs, B ; Kurrle, R ; Resch, K</creatorcontrib><description>Human PBL activated with anti-TCR/CD3 mAb express high affinity receptors for IL-2, synthesize IL-2, and subsequently proliferate. In contrast, lymphocytes activated by dioctanoylglycerol (DiC8) and ionomycin express high affinity receptors; however, no IL-2 synthesis is detectable. Anti-TCR/CD3 antibodies, as well as DiC8 cause translocation of protein kinase C (PKC) from the cytosol to the plasma membrane. In DiC8-stimulated cells translocation of PKC is detectable after 15 min, then it declines to control levels. In lymphocytes activated by antiTCR/CD3 mAb translocation of PKC is detectable after 15 min, then it declines to control levels, followed by a second, long lasting activation of the enzyme up to 4 h. Addition of polyunsaturated fatty acids to DiC8 + ionomycin-treated cells leads to IL-2 synthesis and proliferation. Incorporation of poly-unsaturated fatty acids into plasma membrane phospholipids results in long term activation of PKC. The results suggest that elevated incorporation of polyunsaturated fatty acids and thus continuous activation and translocation of PKC represents a necessary early signal for IL-2 synthesis and proliferation in human lymphocytes.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.143.9.2806</identifier><identifier>PMID: 2530278</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine ; Antigens, Differentiation, T-Lymphocyte - physiology ; CD3 Complex ; Cells, Cultured ; Diglycerides - pharmacology ; DNA - biosynthesis ; fatty acids ; Fatty Acids, Unsaturated - physiology ; Humans ; In Vitro Techniques ; Indomethacin - pharmacology ; Interleukin-2 - biosynthesis ; Ionomycin - pharmacology ; Isoquinolines - pharmacology ; Linoleic Acid ; Linoleic Acids - pharmacology ; Lymphocyte Activation - drug effects ; Lymphocytes - immunology ; Masoprocol - pharmacology ; Membrane Lipids - physiology ; phospholipids ; Phospholipids - physiology ; Piperazines - pharmacology ; plasma membranes ; Protein Kinase C - physiology ; Receptors, Antigen, T-Cell - physiology ; Sphingosine - pharmacology</subject><ispartof>The Journal of immunology (1950), 1989-11, Vol.143 (9), p.2806-2813</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3236-a920ab0856b9a7c88c026e435eec73b04474c96f0bf8dd8183b453aa5912aab43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2530278$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Szamel, M</creatorcontrib><creatorcontrib>Rehermann, B</creatorcontrib><creatorcontrib>Krebs, B</creatorcontrib><creatorcontrib>Kurrle, R</creatorcontrib><creatorcontrib>Resch, K</creatorcontrib><title>Activation signals in human lymphocytes. Incorporation of polyunsaturated fatty acids into plasma membrane phospholipids regulates IL-2 synthesis via sustained activation of protein kinase C</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>Human PBL activated with anti-TCR/CD3 mAb express high affinity receptors for IL-2, synthesize IL-2, and subsequently proliferate. In contrast, lymphocytes activated by dioctanoylglycerol (DiC8) and ionomycin express high affinity receptors; however, no IL-2 synthesis is detectable. Anti-TCR/CD3 antibodies, as well as DiC8 cause translocation of protein kinase C (PKC) from the cytosol to the plasma membrane. In DiC8-stimulated cells translocation of PKC is detectable after 15 min, then it declines to control levels. In lymphocytes activated by antiTCR/CD3 mAb translocation of PKC is detectable after 15 min, then it declines to control levels, followed by a second, long lasting activation of the enzyme up to 4 h. Addition of polyunsaturated fatty acids to DiC8 + ionomycin-treated cells leads to IL-2 synthesis and proliferation. Incorporation of poly-unsaturated fatty acids into plasma membrane phospholipids results in long term activation of PKC. The results suggest that elevated incorporation of polyunsaturated fatty acids and thus continuous activation and translocation of PKC represents a necessary early signal for IL-2 synthesis and proliferation in human lymphocytes.</description><subject>1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine</subject><subject>Antigens, Differentiation, T-Lymphocyte - physiology</subject><subject>CD3 Complex</subject><subject>Cells, Cultured</subject><subject>Diglycerides - pharmacology</subject><subject>DNA - biosynthesis</subject><subject>fatty acids</subject><subject>Fatty Acids, Unsaturated - physiology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Indomethacin - pharmacology</subject><subject>Interleukin-2 - biosynthesis</subject><subject>Ionomycin - pharmacology</subject><subject>Isoquinolines - pharmacology</subject><subject>Linoleic Acid</subject><subject>Linoleic Acids - pharmacology</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocytes - immunology</subject><subject>Masoprocol - pharmacology</subject><subject>Membrane Lipids - physiology</subject><subject>phospholipids</subject><subject>Phospholipids - physiology</subject><subject>Piperazines - pharmacology</subject><subject>plasma membranes</subject><subject>Protein Kinase C - physiology</subject><subject>Receptors, Antigen, T-Cell - physiology</subject><subject>Sphingosine - pharmacology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O0zAUhSMEGsrAEyAkr2CV4L848XJU8VOpEhtYWzeu03hw7BA7U-Xl5tlw1TKwY2FZ8v3Oucc6RfGW4IpjLj_e23FcfHAV4aySFW2xeFZsSF3jUggsnhcbjCktSSOal8WrGO8xxgJTflPc0Jph2rSb4vFOJ_sAyQaPoj16cBFZj4ZlBI_cOk5D0GsysUI7r8M8hfnChh5Nwa2Lj5CW_GYOqIeUVgTaHs4WKaDJQRwBjWbsZvAGZa-Yj7PTGZnNcXFZGNFuX1IUV58GE21EDxZQXGIC67Mr_M133jmHZHK-n9ZDNGj7unjR58jmzfW-LX58_vR9-7Xcf_uy297tS80oEyVIiqHDbS06CY1uW42pMJzVxuiGdZjzhmspetz17eHQkpZ1vGYAtSQUoOPstnh_8c0Bfi0mJjXaqI1z-V9hiaqRVBIixX9BUrOWS1FnkF1APYcYZ9OrabYjzKsiWJ3rVX_qVbleJdW53qx6d7VfutEcnjTXPvP8w2U-2ONwsrNRuQHnMk3U6XT6x-k3YKa2cQ</recordid><startdate>19891101</startdate><enddate>19891101</enddate><creator>Szamel, M</creator><creator>Rehermann, B</creator><creator>Krebs, B</creator><creator>Kurrle, R</creator><creator>Resch, K</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19891101</creationdate><title>Activation signals in human lymphocytes. Incorporation of polyunsaturated fatty acids into plasma membrane phospholipids regulates IL-2 synthesis via sustained activation of protein kinase C</title><author>Szamel, M ; Rehermann, B ; Krebs, B ; Kurrle, R ; Resch, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3236-a920ab0856b9a7c88c026e435eec73b04474c96f0bf8dd8183b453aa5912aab43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine</topic><topic>Antigens, Differentiation, T-Lymphocyte - physiology</topic><topic>CD3 Complex</topic><topic>Cells, Cultured</topic><topic>Diglycerides - pharmacology</topic><topic>DNA - biosynthesis</topic><topic>fatty acids</topic><topic>Fatty Acids, Unsaturated - physiology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Indomethacin - pharmacology</topic><topic>Interleukin-2 - biosynthesis</topic><topic>Ionomycin - pharmacology</topic><topic>Isoquinolines - pharmacology</topic><topic>Linoleic Acid</topic><topic>Linoleic Acids - pharmacology</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocytes - immunology</topic><topic>Masoprocol - pharmacology</topic><topic>Membrane Lipids - physiology</topic><topic>phospholipids</topic><topic>Phospholipids - physiology</topic><topic>Piperazines - pharmacology</topic><topic>plasma membranes</topic><topic>Protein Kinase C - physiology</topic><topic>Receptors, Antigen, T-Cell - physiology</topic><topic>Sphingosine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Szamel, M</creatorcontrib><creatorcontrib>Rehermann, B</creatorcontrib><creatorcontrib>Krebs, B</creatorcontrib><creatorcontrib>Kurrle, R</creatorcontrib><creatorcontrib>Resch, K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Szamel, M</au><au>Rehermann, B</au><au>Krebs, B</au><au>Kurrle, R</au><au>Resch, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation signals in human lymphocytes. Incorporation of polyunsaturated fatty acids into plasma membrane phospholipids regulates IL-2 synthesis via sustained activation of protein kinase C</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1989-11-01</date><risdate>1989</risdate><volume>143</volume><issue>9</issue><spage>2806</spage><epage>2813</epage><pages>2806-2813</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>Human PBL activated with anti-TCR/CD3 mAb express high affinity receptors for IL-2, synthesize IL-2, and subsequently proliferate. In contrast, lymphocytes activated by dioctanoylglycerol (DiC8) and ionomycin express high affinity receptors; however, no IL-2 synthesis is detectable. Anti-TCR/CD3 antibodies, as well as DiC8 cause translocation of protein kinase C (PKC) from the cytosol to the plasma membrane. In DiC8-stimulated cells translocation of PKC is detectable after 15 min, then it declines to control levels. In lymphocytes activated by antiTCR/CD3 mAb translocation of PKC is detectable after 15 min, then it declines to control levels, followed by a second, long lasting activation of the enzyme up to 4 h. Addition of polyunsaturated fatty acids to DiC8 + ionomycin-treated cells leads to IL-2 synthesis and proliferation. Incorporation of poly-unsaturated fatty acids into plasma membrane phospholipids results in long term activation of PKC. The results suggest that elevated incorporation of polyunsaturated fatty acids and thus continuous activation and translocation of PKC represents a necessary early signal for IL-2 synthesis and proliferation in human lymphocytes.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>2530278</pmid><doi>10.4049/jimmunol.143.9.2806</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Antigens, Differentiation, T-Lymphocyte - physiology CD3 Complex Cells, Cultured Diglycerides - pharmacology DNA - biosynthesis fatty acids Fatty Acids, Unsaturated - physiology Humans In Vitro Techniques Indomethacin - pharmacology Interleukin-2 - biosynthesis Ionomycin - pharmacology Isoquinolines - pharmacology Linoleic Acid Linoleic Acids - pharmacology Lymphocyte Activation - drug effects Lymphocytes - immunology Masoprocol - pharmacology Membrane Lipids - physiology phospholipids Phospholipids - physiology Piperazines - pharmacology plasma membranes Protein Kinase C - physiology Receptors, Antigen, T-Cell - physiology Sphingosine - pharmacology |
title | Activation signals in human lymphocytes. Incorporation of polyunsaturated fatty acids into plasma membrane phospholipids regulates IL-2 synthesis via sustained activation of protein kinase C |
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