Proteomic 'contigs' of Ochrobactrum anthropi, application of extensive pH gradients

The most extensive linear pH gradients yet employed in combination with two‐dimensional gel electrophoresis are described, along with their application in proteome analysis. A significant proportion of the protein compliment of bacterial species is believed to be accessible using an extended linear...

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Veröffentlicht in:	Electrophoresis 1997-08, Vol.18 (8), p.1373-1383
Hauptverfasser:	Wasinger, Valerie C., Bjellqvist, Bengt, Humphery-Smith, Ian
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Contiguous windows of protein expression Electrophoresis, Gel, Two-Dimensional - methods Genome Genome, Bacterial Gram-Negative Bacteria - chemistry Gram-Negative Bacteria - genetics Immobilised pH gradients Isoelectric Point Molecular Sequence Data Molecular Weight Peptide Mapping - methods Proteome Two-dimensional polyacrylamide gel electrophoresis
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