Alteration in NMDA receptor subunit mRNA expression in vulnerable and resistant regions of in vitro ischemic rat hippocampal slices
Brain insults, including cerebral ischemia, can alter glutamate receptor subunit expression in vulnerable neurons. Understanding these post-ischemic changes in glutamate receptors could enhance our ability to identify specific, novel neuroprotective compounds. Reverse transcription-polymerase chain...
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| Veröffentlicht in: | Neuroscience letters 1997-08, Vol.232 (2), p.87-90 |
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| container_title | Neuroscience letters |
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| creator | Small, Daniel L Poulter, Michael O Buchan, Alastair M Morley, Paul |
| description | Brain insults, including cerebral ischemia, can alter glutamate receptor subunit expression in vulnerable neurons. Understanding these post-ischemic changes in glutamate receptors could enhance our ability to identify specific, novel neuroprotective compounds. Reverse transcription-polymerase chain reaction (RT-PCR) amplification was used to quantify the altered expression of the N-methyl-d-aspartate (NMDA) NR2A, NR2B and NR2C subunits relative to one another in rat hippocampal slices in resistant and vulnerable regions following in vitro oxygen-glucose deprivation. Ninety minutes after re-oxygenation and return to 10 mM glucose, there was a significant increase in the expression of NR2C relative to NR2B and NR2A in the slice as a whole, as well as in the selectively vulnerable CA1 region and the resistant CA3 and dentate gyrus regions. |
| doi_str_mv | 10.1016/S0304-3940(97)00592-2 |
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Understanding these post-ischemic changes in glutamate receptors could enhance our ability to identify specific, novel neuroprotective compounds. Reverse transcription-polymerase chain reaction (RT-PCR) amplification was used to quantify the altered expression of the N-methyl-d-aspartate (NMDA) NR2A, NR2B and NR2C subunits relative to one another in rat hippocampal slices in resistant and vulnerable regions following in vitro oxygen-glucose deprivation. 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Poulter, Michael O ; Buchan, Alastair M ; Morley, Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-dd83117e0b9433ac36ae8acf76d445daff2e99e196501a7688e92a84608114c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Excitotoxicity</topic><topic>Hippocampus</topic><topic>Hippocampus - blood supply</topic><topic>Hippocampus - metabolism</topic><topic>In vitro ischemia</topic><topic>In Vitro Techniques</topic><topic>Ischemia - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>N-Methyl-d-aspartate receptor</topic><topic>Neurology</topic><topic>Polymerase Chain Reaction</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, N-Methyl-D-Aspartate - metabolism</topic><topic>Reverse transcription-polymerase chain reaction</topic><topic>RNA, Messenger - analysis</topic><topic>Time Factors</topic><topic>Vascular diseases and vascular malformations of the nervous system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Small, Daniel L</creatorcontrib><creatorcontrib>Poulter, Michael O</creatorcontrib><creatorcontrib>Buchan, Alastair M</creatorcontrib><creatorcontrib>Morley, Paul</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Small, Daniel L</au><au>Poulter, Michael O</au><au>Buchan, Alastair M</au><au>Morley, Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alteration in NMDA receptor subunit mRNA expression in vulnerable and resistant regions of in vitro ischemic rat hippocampal slices</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1997-08-29</date><risdate>1997</risdate><volume>232</volume><issue>2</issue><spage>87</spage><epage>90</epage><pages>87-90</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Brain insults, including cerebral ischemia, can alter glutamate receptor subunit expression in vulnerable neurons. Understanding these post-ischemic changes in glutamate receptors could enhance our ability to identify specific, novel neuroprotective compounds. Reverse transcription-polymerase chain reaction (RT-PCR) amplification was used to quantify the altered expression of the N-methyl-d-aspartate (NMDA) NR2A, NR2B and NR2C subunits relative to one another in rat hippocampal slices in resistant and vulnerable regions following in vitro oxygen-glucose deprivation. Ninety minutes after re-oxygenation and return to 10 mM glucose, there was a significant increase in the expression of NR2C relative to NR2B and NR2A in the slice as a whole, as well as in the selectively vulnerable CA1 region and the resistant CA3 and dentate gyrus regions.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>9302093</pmid><doi>10.1016/S0304-3940(97)00592-2</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3940 |
| ispartof | Neuroscience letters, 1997-08, Vol.232 (2), p.87-90 |
| issn | 0304-3940 1872-7972 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Excitotoxicity Hippocampus Hippocampus - blood supply Hippocampus - metabolism In vitro ischemia In Vitro Techniques Ischemia - metabolism Male Medical sciences N-Methyl-d-aspartate receptor Neurology Polymerase Chain Reaction Rats Rats, Wistar Receptors, N-Methyl-D-Aspartate - metabolism Reverse transcription-polymerase chain reaction RNA, Messenger - analysis Time Factors Vascular diseases and vascular malformations of the nervous system |
| title | Alteration in NMDA receptor subunit mRNA expression in vulnerable and resistant regions of in vitro ischemic rat hippocampal slices |
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