Capillary Electrophoresis with Laser-Induced Fluorescence Detection for the Analysis of Free and Immune-Complexed Green Fluorescent Protein

Naturally fluorescing green fluorescent protein (GFP) was separated by capillary electrophoresis (CE) and detected by laser-induced fluorescence (LIF). Exploiting recombinant technology and the natural fluorescence of GFP presents the capability of preempting the need for fluorescent derivatization....

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Veröffentlicht in:	Analytical biochemistry 1997-09, Vol.251 (2), p.210-218
Hauptverfasser:	Korf, Gina M., Landers, James P., O'kane, Dennis J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigen-Antibody Complex - analysis Antigen-Antibody Complex - immunology Electrophoresis, Capillary - methods Green Fluorescent Proteins Hydrogen-Ion Concentration Lasers Luminescent Proteins - analysis Luminescent Proteins - immunology Mice Protein Binding Putrescine Rabbits Scyphozoa Spectrometry, Fluorescence
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