Nutritional Requirements of Papillomavirus-Transformed Mouse Cells and an Uninfected Parent Line in Serum-Free Culture
A serum-free culture system was used to compare the nutritional requirements of mouse mammary cells transformed by bovine papillomavirus type 1 (ID13 cells) and the uninfected parent line (C127 cells). The serum-free, chemically defined medium used for this study was an MCDB 151-based medium (MCDB 1...
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| Veröffentlicht in: | In vitro 1989-10, Vol.25 (10), p.909-913 |
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| creator | Di Lorenzo, Teresa P. De Maro, Joseph A. Pumo, Dorothy E. |
| description | A serum-free culture system was used to compare the nutritional requirements of mouse mammary cells transformed by bovine papillomavirus type 1 (ID13 cells) and the uninfected parent line (C127 cells). The serum-free, chemically defined medium used for this study was an MCDB 151-based medium (MCDB 151+S+I), supplemented with epidermal growth factor, transferrin, hydrocortisone, ethanolamine, phosphoethanolamine, retinoic acid, trace metals, and insulin. Proliferation of either cell type in serum-free culture required the addition of 250 μg/ml of insulin. ID13 cells have a doubling time of greater than 96 h in MCDB 151+S+I, whereas C127 cells have a doubling time of 60 h. This is in sharp contrast to the growth characteristics of the two cell types in 10% fetal bovine serum, where doubling times for the ID13 and C127 cells are 24 and 30 h, respectively. Culture of the cells in a serum-free medium has therefore revealed that the papillomavirus-transformed cells have more stringent growth requirements than the uninfected parent line. |
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The serum-free, chemically defined medium used for this study was an MCDB 151-based medium (MCDB 151+S+I), supplemented with epidermal growth factor, transferrin, hydrocortisone, ethanolamine, phosphoethanolamine, retinoic acid, trace metals, and insulin. Proliferation of either cell type in serum-free culture required the addition of 250 μg/ml of insulin. ID13 cells have a doubling time of greater than 96 h in MCDB 151+S+I, whereas C127 cells have a doubling time of 60 h. This is in sharp contrast to the growth characteristics of the two cell types in 10% fetal bovine serum, where doubling times for the ID13 and C127 cells are 24 and 30 h, respectively. Culture of the cells in a serum-free medium has therefore revealed that the papillomavirus-transformed cells have more stringent growth requirements than the uninfected parent line.</description><identifier>ISSN: 0883-8364</identifier><identifier>ISSN: 0073-5655</identifier><identifier>EISSN: 2327-431X</identifier><identifier>PMID: 2553658</identifier><identifier>CODEN: ICDBEO</identifier><language>eng</language><publisher>Largo, MD: Tissue Culture Association, Inc</publisher><subject>Animal cells ; Animals ; Biological and medical sciences ; Biotechnology ; Calcium ; Cell culture techniques ; Cell cultures. Hybridization. Fusion ; Cell growth ; Cell Line, Transformed ; Cell lines ; Cell Transformation, Viral - drug effects ; Cell Transformation, Viral - physiology ; Culture Media - pharmacology ; Cultured cells ; Dose-Response Relationship, Drug ; Epidermal cells ; Epidermal Growth Factor - pharmacology ; Epithelial cells ; Establishment of new cell lines, improvement of cultural methods, mass cultures ; Ethanolamines - pharmacology ; Eukaryotic cell cultures ; Female ; Fundamental and applied biological sciences. Psychology ; Hydrocortisone - pharmacology ; Insulin ; Insulin - pharmacology ; Mammary Glands, Animal - cytology ; Mammary Glands, Animal - pathology ; Mammary Glands, Animal - physiology ; Metals - pharmacology ; Methods. Procedures. Technologies ; Mice ; Molecular and cellular biology ; Nutritional Requirements ; Papillomaviridae ; Transferrin - pharmacology ; Transformed cell line ; Tretinoin - pharmacology ; Ungulates</subject><ispartof>In vitro, 1989-10, Vol.25 (10), p.909-913</ispartof><rights>Copyright 1989 Tissue Culture Association, Inc.</rights><rights>1990 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/20171528$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/20171528$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6690164$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2553658$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Di Lorenzo, Teresa P.</creatorcontrib><creatorcontrib>De Maro, Joseph A.</creatorcontrib><creatorcontrib>Pumo, Dorothy E.</creatorcontrib><title>Nutritional Requirements of Papillomavirus-Transformed Mouse Cells and an Uninfected Parent Line in Serum-Free Culture</title><title>In vitro</title><addtitle>In Vitro Cell Dev Biol</addtitle><description>A serum-free culture system was used to compare the nutritional requirements of mouse mammary cells transformed by bovine papillomavirus type 1 (ID13 cells) and the uninfected parent line (C127 cells). The serum-free, chemically defined medium used for this study was an MCDB 151-based medium (MCDB 151+S+I), supplemented with epidermal growth factor, transferrin, hydrocortisone, ethanolamine, phosphoethanolamine, retinoic acid, trace metals, and insulin. Proliferation of either cell type in serum-free culture required the addition of 250 μg/ml of insulin. ID13 cells have a doubling time of greater than 96 h in MCDB 151+S+I, whereas C127 cells have a doubling time of 60 h. This is in sharp contrast to the growth characteristics of the two cell types in 10% fetal bovine serum, where doubling times for the ID13 and C127 cells are 24 and 30 h, respectively. Culture of the cells in a serum-free medium has therefore revealed that the papillomavirus-transformed cells have more stringent growth requirements than the uninfected parent line.</description><subject>Animal cells</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Calcium</subject><subject>Cell culture techniques</subject><subject>Cell cultures. Hybridization. Fusion</subject><subject>Cell growth</subject><subject>Cell Line, Transformed</subject><subject>Cell lines</subject><subject>Cell Transformation, Viral - drug effects</subject><subject>Cell Transformation, Viral - physiology</subject><subject>Culture Media - pharmacology</subject><subject>Cultured cells</subject><subject>Dose-Response Relationship, Drug</subject><subject>Epidermal cells</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Epithelial cells</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Ethanolamines - pharmacology</subject><subject>Eukaryotic cell cultures</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrocortisone - pharmacology</subject><subject>Insulin</subject><subject>Insulin - pharmacology</subject><subject>Mammary Glands, Animal - cytology</subject><subject>Mammary Glands, Animal - pathology</subject><subject>Mammary Glands, Animal - physiology</subject><subject>Metals - pharmacology</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Nutritional Requirements</subject><subject>Papillomaviridae</subject><subject>Transferrin - pharmacology</subject><subject>Transformed cell line</subject><subject>Tretinoin - pharmacology</subject><subject>Ungulates</subject><issn>0883-8364</issn><issn>0073-5655</issn><issn>2327-431X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9Lw0AQxYMotVY_grAH8RbYP9nN5ijFqlC1aAvewiaZhS3Jpt3NFvz2bjHYo4dhBt5vHjPvLJlSRvM0Y-TrPJliKVkqmcgukyvvtxgzLCidJBPKORNcTpPDWxicGUxvVYs-YB-Mgw7s4FGv0UrtTNv2nToYF3y6dsp63bsOGvTaBw9oDm3rkbJNLLSxxmqoh6iulIseaGksIGPRJ7jQpQsHcSO0Q3BwnVxo1Xq4Gfss2Swe1_PndPn-9DJ_WKZbSvGQVlVOc4IFIw1jOCecNnFqmG64xDnXLKt5RhpQTGrMdS5ANRWFQtQUZCaBzZL7X9-d6_cB_FB2xtfxamUhflDmBZWc4PxfkHAmi4IewdsRDFUMotw50yn3XY6JRv1u1JWvVatjZrXxf5gQBSYiO9ls_dC7kwsmxy8l-wFs1Ypz</recordid><startdate>19891001</startdate><enddate>19891001</enddate><creator>Di Lorenzo, Teresa P.</creator><creator>De Maro, Joseph A.</creator><creator>Pumo, Dorothy E.</creator><general>Tissue Culture Association, Inc</general><general>Society for In Vitro Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19891001</creationdate><title>Nutritional Requirements of Papillomavirus-Transformed Mouse Cells and an Uninfected Parent Line in Serum-Free Culture</title><author>Di Lorenzo, Teresa P. ; De Maro, Joseph A. ; Pumo, Dorothy E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j220t-bb72710631d3307152d1d3d3fd58075f34c541dea38f05f76eadb2e96c2e848e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animal cells</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Calcium</topic><topic>Cell culture techniques</topic><topic>Cell cultures. Hybridization. Fusion</topic><topic>Cell growth</topic><topic>Cell Line, Transformed</topic><topic>Cell lines</topic><topic>Cell Transformation, Viral - drug effects</topic><topic>Cell Transformation, Viral - physiology</topic><topic>Culture Media - pharmacology</topic><topic>Cultured cells</topic><topic>Dose-Response Relationship, Drug</topic><topic>Epidermal cells</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Epithelial cells</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass cultures</topic><topic>Ethanolamines - pharmacology</topic><topic>Eukaryotic cell cultures</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrocortisone - pharmacology</topic><topic>Insulin</topic><topic>Insulin - pharmacology</topic><topic>Mammary Glands, Animal - cytology</topic><topic>Mammary Glands, Animal - pathology</topic><topic>Mammary Glands, Animal - physiology</topic><topic>Metals - pharmacology</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Nutritional Requirements</topic><topic>Papillomaviridae</topic><topic>Transferrin - pharmacology</topic><topic>Transformed cell line</topic><topic>Tretinoin - pharmacology</topic><topic>Ungulates</topic><toplevel>online_resources</toplevel><creatorcontrib>Di Lorenzo, Teresa P.</creatorcontrib><creatorcontrib>De Maro, Joseph A.</creatorcontrib><creatorcontrib>Pumo, Dorothy E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>In vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Di Lorenzo, Teresa P.</au><au>De Maro, Joseph A.</au><au>Pumo, Dorothy E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nutritional Requirements of Papillomavirus-Transformed Mouse Cells and an Uninfected Parent Line in Serum-Free Culture</atitle><jtitle>In vitro</jtitle><addtitle>In Vitro Cell Dev Biol</addtitle><date>1989-10-01</date><risdate>1989</risdate><volume>25</volume><issue>10</issue><spage>909</spage><epage>913</epage><pages>909-913</pages><issn>0883-8364</issn><issn>0073-5655</issn><eissn>2327-431X</eissn><coden>ICDBEO</coden><abstract>A serum-free culture system was used to compare the nutritional requirements of mouse mammary cells transformed by bovine papillomavirus type 1 (ID13 cells) and the uninfected parent line (C127 cells). The serum-free, chemically defined medium used for this study was an MCDB 151-based medium (MCDB 151+S+I), supplemented with epidermal growth factor, transferrin, hydrocortisone, ethanolamine, phosphoethanolamine, retinoic acid, trace metals, and insulin. Proliferation of either cell type in serum-free culture required the addition of 250 μg/ml of insulin. ID13 cells have a doubling time of greater than 96 h in MCDB 151+S+I, whereas C127 cells have a doubling time of 60 h. This is in sharp contrast to the growth characteristics of the two cell types in 10% fetal bovine serum, where doubling times for the ID13 and C127 cells are 24 and 30 h, respectively. Culture of the cells in a serum-free medium has therefore revealed that the papillomavirus-transformed cells have more stringent growth requirements than the uninfected parent line.</abstract><cop>Largo, MD</cop><pub>Tissue Culture Association, Inc</pub><pmid>2553658</pmid><tpages>5</tpages></addata></record> |
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| source | Jstor Complete Legacy; MEDLINE |
| subjects | Animal cells Animals Biological and medical sciences Biotechnology Calcium Cell culture techniques Cell cultures. Hybridization. Fusion Cell growth Cell Line, Transformed Cell lines Cell Transformation, Viral - drug effects Cell Transformation, Viral - physiology Culture Media - pharmacology Cultured cells Dose-Response Relationship, Drug Epidermal cells Epidermal Growth Factor - pharmacology Epithelial cells Establishment of new cell lines, improvement of cultural methods, mass cultures Ethanolamines - pharmacology Eukaryotic cell cultures Female Fundamental and applied biological sciences. Psychology Hydrocortisone - pharmacology Insulin Insulin - pharmacology Mammary Glands, Animal - cytology Mammary Glands, Animal - pathology Mammary Glands, Animal - physiology Metals - pharmacology Methods. Procedures. Technologies Mice Molecular and cellular biology Nutritional Requirements Papillomaviridae Transferrin - pharmacology Transformed cell line Tretinoin - pharmacology Ungulates |
| title | Nutritional Requirements of Papillomavirus-Transformed Mouse Cells and an Uninfected Parent Line in Serum-Free Culture |
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