Computer-assisted semen analysis: results vary across technicians who prepare videotapes

Automated semen analyses revealed differences of 21% to 30% in concentration-related parameters and 5% to 11% in motion-related parameters between means of groups of replicate specimens. Disparities among videotapes produced by two laboratory technicians accounted for the divergence in concentration...

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Veröffentlicht in:Fertility and sterility 1989-10, Vol.52 (4), p.673-677
Hauptverfasser: Levine, Richard J., Mathew, Ravi M., Brown, Michelle H., Hurtt, Mark E., Bentley, Karin S., Mohr, Kathleen L., Working, Peter K.
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container_end_page 677
container_issue 4
container_start_page 673
container_title Fertility and sterility
container_volume 52
creator Levine, Richard J.
Mathew, Ravi M.
Brown, Michelle H.
Hurtt, Mark E.
Bentley, Karin S.
Mohr, Kathleen L.
Working, Peter K.
description Automated semen analyses revealed differences of 21% to 30% in concentration-related parameters and 5% to 11% in motion-related parameters between means of groups of replicate specimens. Disparities among videotapes produced by two laboratory technicians accounted for the divergence in concentration-related parameters. This resulted partially from differences between the two technicians in propensity to dilute concentrated specimens. The causes of the greater portion of disparities between videotaping technicians, however, have not been identified. Differences in motion-related parameters could not be ascribed to technicians, but the basis for these differences is also unknown. The results suggest that values obtained from the CellSoft image analysis system may not be comparable between technicians or laboratories, despite use of identical computer parameter settings. Until effective quality control measures have been implemented, such comparisons must be made with caution.
doi_str_mv 10.1016/S0015-0282(16)60985-8
format Article
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source MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection
subjects Biological and medical sciences
Diagnosis, Computer-Assisted
Gynecology. Andrology. Obstetrics
Humans
Male
Male genital diseases
Medical sciences
Non tumoral diseases
Observer Variation
Seasons
Semen - cytology
Sperm Count
Sperm Motility
Videotape Recording
title Computer-assisted semen analysis: results vary across technicians who prepare videotapes
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