Differential Inhibitory Effects of Various Herb Extracts on the Activities of Reverse Transcriptase and Various Deoxyribonucleic Acid (DNA) Polymerases
Forty preparations of the extracts from 28 kinds of Asian herbs were tested for ability to inhibit the activities of murine retroviral reverse transcriptase and human deoxyribonucleic acid (DNA) polymerases. Among the 40 extracts, 35 inhibited reverse transcriptase activity and 29 inhibited DNA poly...
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| Veröffentlicht in: | Chemical & pharmaceutical bulletin 1989/07/25, Vol.37(7), pp.1810-1812 |
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| container_title | Chemical & pharmaceutical bulletin |
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| creator | ONO, Katsuhiko NAKANE, Hideo MENG, Zeng-Mu OSE, Youki SAKAI, Yoshimichi MIZUNO, Mizuo |
| description | Forty preparations of the extracts from 28 kinds of Asian herbs were tested for ability to inhibit the activities of murine retroviral reverse transcriptase and human deoxyribonucleic acid (DNA) polymerases. Among the 40 extracts, 35 inhibited reverse transcriptase activity and 29 inhibited DNA polymerase α activity. The inhibitory potencies of these extracts were expressed as the 50% inhibition concentrations (IC50), at which the enzyme activities were inhibited by 50%. Very strong inhibitions were observed with the extracts from Millettia pachycarpa (Leguminosae) and Mallotus apelta (Euphorbiaceae) as shown by their low IC50 values for reverse transcriptase (0.4-0.5 μg/ml) and DNA polymerase α(0.9-1.4 μg/ml). Enzyme kinetic analysis revealed that the mode of inhibition of reverse transcriptase by these two extracts was competitive with respect to the template·primer [poly(rA)·oligo(dT)] and noncompetitive with respect to deoxythymidine triphosphate (dTTP) substrate. Besides reverse transcriptase and DNA polymerase α, DNA polymerase I and ribonucleic acid (RNA) polymerase from E. coli were inhibited by these two extracts. These results indicate that the herb extracts contain as yet unidentified substance(s) which inhibit the activities of reverse transcriptase and cellular DNA polymerases. |
| doi_str_mv | 10.1248/cpb.37.1810 |
| format | Article |
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Among the 40 extracts, 35 inhibited reverse transcriptase activity and 29 inhibited DNA polymerase α activity. The inhibitory potencies of these extracts were expressed as the 50% inhibition concentrations (IC50), at which the enzyme activities were inhibited by 50%. Very strong inhibitions were observed with the extracts from Millettia pachycarpa (Leguminosae) and Mallotus apelta (Euphorbiaceae) as shown by their low IC50 values for reverse transcriptase (0.4-0.5 μg/ml) and DNA polymerase α(0.9-1.4 μg/ml). Enzyme kinetic analysis revealed that the mode of inhibition of reverse transcriptase by these two extracts was competitive with respect to the template·primer [poly(rA)·oligo(dT)] and noncompetitive with respect to deoxythymidine triphosphate (dTTP) substrate. Besides reverse transcriptase and DNA polymerase α, DNA polymerase I and ribonucleic acid (RNA) polymerase from E. coli were inhibited by these two extracts. These results indicate that the herb extracts contain as yet unidentified substance(s) which inhibit the activities of reverse transcriptase and cellular DNA polymerases.</description><identifier>ISSN: 0009-2363</identifier><identifier>EISSN: 1347-5223</identifier><identifier>DOI: 10.1248/cpb.37.1810</identifier><identifier>PMID: 2478305</identifier><identifier>CODEN: CPBTAL</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antiviral agents ; Biological and medical sciences ; DNA polymerase ; enzyme inhibition ; herb extract ; Humans ; KB Cells ; Mallotus apelta ; Medical sciences ; Millettia pachycarpa ; Nucleic Acid Synthesis Inhibitors ; Pharmacology. Drug treatments ; Plant Extracts - pharmacology ; reverse transcriptase ; Reverse Transcriptase Inhibitors</subject><ispartof>Chemical and Pharmaceutical Bulletin, 1989/07/25, Vol.37(7), pp.1810-1812</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1991 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1989</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c607t-261d431b590b5978d8df6f19bbdd81aacf06707997ea3b3969013e5d2d4f7db83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,1877,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19583443$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2478305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ONO, Katsuhiko</creatorcontrib><creatorcontrib>NAKANE, Hideo</creatorcontrib><creatorcontrib>MENG, Zeng-Mu</creatorcontrib><creatorcontrib>OSE, Youki</creatorcontrib><creatorcontrib>SAKAI, Yoshimichi</creatorcontrib><creatorcontrib>MIZUNO, Mizuo</creatorcontrib><title>Differential Inhibitory Effects of Various Herb Extracts on the Activities of Reverse Transcriptase and Various Deoxyribonucleic Acid (DNA) Polymerases</title><title>Chemical & pharmaceutical bulletin</title><addtitle>Chem. Pharm. Bull.</addtitle><description>Forty preparations of the extracts from 28 kinds of Asian herbs were tested for ability to inhibit the activities of murine retroviral reverse transcriptase and human deoxyribonucleic acid (DNA) polymerases. Among the 40 extracts, 35 inhibited reverse transcriptase activity and 29 inhibited DNA polymerase α activity. The inhibitory potencies of these extracts were expressed as the 50% inhibition concentrations (IC50), at which the enzyme activities were inhibited by 50%. Very strong inhibitions were observed with the extracts from Millettia pachycarpa (Leguminosae) and Mallotus apelta (Euphorbiaceae) as shown by their low IC50 values for reverse transcriptase (0.4-0.5 μg/ml) and DNA polymerase α(0.9-1.4 μg/ml). Enzyme kinetic analysis revealed that the mode of inhibition of reverse transcriptase by these two extracts was competitive with respect to the template·primer [poly(rA)·oligo(dT)] and noncompetitive with respect to deoxythymidine triphosphate (dTTP) substrate. Besides reverse transcriptase and DNA polymerase α, DNA polymerase I and ribonucleic acid (RNA) polymerase from E. coli were inhibited by these two extracts. These results indicate that the herb extracts contain as yet unidentified substance(s) which inhibit the activities of reverse transcriptase and cellular DNA polymerases.</description><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antiviral agents</subject><subject>Biological and medical sciences</subject><subject>DNA polymerase</subject><subject>enzyme inhibition</subject><subject>herb extract</subject><subject>Humans</subject><subject>KB Cells</subject><subject>Mallotus apelta</subject><subject>Medical sciences</subject><subject>Millettia pachycarpa</subject><subject>Nucleic Acid Synthesis Inhibitors</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - pharmacology</subject><subject>reverse transcriptase</subject><subject>Reverse Transcriptase Inhibitors</subject><issn>0009-2363</issn><issn>1347-5223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkV-LEzEUxYMoa1198lkIiLIirfkzM5k8lm3XXVhUZPU1ZJIbmzKd6SaZZftJ_Lpm2tIFH5KQnF_OvdyD0FtKZpQV9RezbWZczGhNyTM0obwQ05Ix_hxNCCFyynjFX6JXMa4JYSUR_AydsULUnJQT9HfhnYMAXfK6xTfdyjc-9WGHl_nZpIh7h3_r4Psh4msIDV4-pqD3QofTCvDcJP_gk4c9-hMeIETAd0F30QS_TTrfdGdPJgvoH3fBN303mBa8yQbe4ovFt_kn_KNvdxsI-Ut8jV443UZ4czzP0a-r5d3l9fT2-9eby_nt1FREpCmrqC04bUpJ8hK1ra2rHJVNY21NtTaOVIIIKQVo3nBZSUI5lJbZwgnb1PwcfTz4bkN_P0BMauOjgbbVHeR2lZCsZrKkGXz_H7juh9Dl3hQtKsI4F3vq84EyoY8xgFPb4Dc67BQlagxL5bAUF2oMK9Pvjp5DswF7Yo_pZP3DUdfR6NbloRofnyxlWfOi4JlbHLh1TPoPnAAdks9DHmuO7FhXHLax_JO80kFBx_8Bo6i15w</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>ONO, Katsuhiko</creator><creator>NAKANE, Hideo</creator><creator>MENG, Zeng-Mu</creator><creator>OSE, Youki</creator><creator>SAKAI, Yoshimichi</creator><creator>MIZUNO, Mizuo</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>Differential Inhibitory Effects of Various Herb Extracts on the Activities of Reverse Transcriptase and Various Deoxyribonucleic Acid (DNA) Polymerases</title><author>ONO, Katsuhiko ; NAKANE, Hideo ; MENG, Zeng-Mu ; OSE, Youki ; SAKAI, Yoshimichi ; MIZUNO, Mizuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c607t-261d431b590b5978d8df6f19bbdd81aacf06707997ea3b3969013e5d2d4f7db83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antiviral agents</topic><topic>Biological and medical sciences</topic><topic>DNA polymerase</topic><topic>enzyme inhibition</topic><topic>herb extract</topic><topic>Humans</topic><topic>KB Cells</topic><topic>Mallotus apelta</topic><topic>Medical sciences</topic><topic>Millettia pachycarpa</topic><topic>Nucleic Acid Synthesis Inhibitors</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - pharmacology</topic><topic>reverse transcriptase</topic><topic>Reverse Transcriptase Inhibitors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ONO, Katsuhiko</creatorcontrib><creatorcontrib>NAKANE, Hideo</creatorcontrib><creatorcontrib>MENG, Zeng-Mu</creatorcontrib><creatorcontrib>OSE, Youki</creatorcontrib><creatorcontrib>SAKAI, Yoshimichi</creatorcontrib><creatorcontrib>MIZUNO, Mizuo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ONO, Katsuhiko</au><au>NAKANE, Hideo</au><au>MENG, Zeng-Mu</au><au>OSE, Youki</au><au>SAKAI, Yoshimichi</au><au>MIZUNO, Mizuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential Inhibitory Effects of Various Herb Extracts on the Activities of Reverse Transcriptase and Various Deoxyribonucleic Acid (DNA) Polymerases</atitle><jtitle>Chemical & pharmaceutical bulletin</jtitle><addtitle>Chem. Pharm. Bull.</addtitle><date>1989</date><risdate>1989</risdate><volume>37</volume><issue>7</issue><spage>1810</spage><epage>1812</epage><pages>1810-1812</pages><issn>0009-2363</issn><eissn>1347-5223</eissn><coden>CPBTAL</coden><abstract>Forty preparations of the extracts from 28 kinds of Asian herbs were tested for ability to inhibit the activities of murine retroviral reverse transcriptase and human deoxyribonucleic acid (DNA) polymerases. Among the 40 extracts, 35 inhibited reverse transcriptase activity and 29 inhibited DNA polymerase α activity. The inhibitory potencies of these extracts were expressed as the 50% inhibition concentrations (IC50), at which the enzyme activities were inhibited by 50%. Very strong inhibitions were observed with the extracts from Millettia pachycarpa (Leguminosae) and Mallotus apelta (Euphorbiaceae) as shown by their low IC50 values for reverse transcriptase (0.4-0.5 μg/ml) and DNA polymerase α(0.9-1.4 μg/ml). Enzyme kinetic analysis revealed that the mode of inhibition of reverse transcriptase by these two extracts was competitive with respect to the template·primer [poly(rA)·oligo(dT)] and noncompetitive with respect to deoxythymidine triphosphate (dTTP) substrate. Besides reverse transcriptase and DNA polymerase α, DNA polymerase I and ribonucleic acid (RNA) polymerase from E. coli were inhibited by these two extracts. These results indicate that the herb extracts contain as yet unidentified substance(s) which inhibit the activities of reverse transcriptase and cellular DNA polymerases.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>2478305</pmid><doi>10.1248/cpb.37.1810</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Chemical and Pharmaceutical Bulletin, 1989/07/25, Vol.37(7), pp.1810-1812 |
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| subjects | Antibiotics. Antiinfectious agents. Antiparasitic agents Antiviral agents Biological and medical sciences DNA polymerase enzyme inhibition herb extract Humans KB Cells Mallotus apelta Medical sciences Millettia pachycarpa Nucleic Acid Synthesis Inhibitors Pharmacology. Drug treatments Plant Extracts - pharmacology reverse transcriptase Reverse Transcriptase Inhibitors |
| title | Differential Inhibitory Effects of Various Herb Extracts on the Activities of Reverse Transcriptase and Various Deoxyribonucleic Acid (DNA) Polymerases |
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