Site-specific radiolabeling of monoclonal antibodies with biotin/streptavidin

Sulfhydryl groups of a murine IgG2a monoclonal antibody, 5G6.4, were site-specifically biotinylated with N-iodo-acetyl- N′-biotinylhexylenediamine (compound 1) following partial reduction of disulfide bonds. Antibody labeling using fluoresceinated or [ 125I]streptavidin were consistent with sulfhydr...

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Veröffentlicht in:	International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology Nuclear medicine and biology, 1989, Vol.16 (5), p.525,529-527,529
Hauptverfasser:	Del Rosario, Renato B., Wahl, Richard L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal Bacterial Proteins Biotin Fluoresceins Iodine Radioisotopes Isotope Labeling - methods Mice Streptavidin Sulfhydryl Compounds
Online-Zugang:	Volltext
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