Isolation of the major basic nuclear protein and its localization on chromosomes of the dinoflagellate, Oxyrrhis marina
Basic nuclear proteins were extracted from isolated nuclei of Oxyrrhis marina. The HPLC pattern of the extract showed a single major peak, which consisted of a major band with an apparent molecular mass of 23 kDa on an SDS-PAGE gel. We designated this protein as Np23 because of its apparent molecula...
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| Veröffentlicht in: | Biology of the cell 1997-03, Vol.89 (1), p.43-52 |
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| container_title | Biology of the cell |
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| creator | Kato, Koichi H Moriyama, Akihiko Huitorel, Philippe Cosson, Jacky Cachon, Monique Sato, Hidemi |
| description | Basic nuclear proteins were extracted from isolated nuclei of
Oxyrrhis marina. The HPLC pattern of the extract showed a single major peak, which consisted of a major band with an apparent molecular mass of 23 kDa on an SDS-PAGE gel. We designated this protein as Np23 because of its apparent molecular mass. The amino acid composition of this protein revealed its extremely basic nature with a high lysine content. Polyclonal antibodies were raised against Np23. Immunofluorescence microscopy showed that Np23 was localized within the nucleus of dividing and non-dividing cells as well, and immuno-electron microscopy showed that the protein was localized only on chromosomes. These data established that Np23 is the major basic chromosome protein of
Oxyrrhis marina. |
| doi_str_mv | 10.1016/S0248-4900(99)80080-X |
| format | Article |
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Oxyrrhis marina.</description><identifier>ISSN: 0248-4900</identifier><identifier>EISSN: 1768-322X</identifier><identifier>DOI: 10.1016/S0248-4900(99)80080-X</identifier><identifier>PMID: 9297782</identifier><language>eng</language><publisher>Oxford, UK: Elsevier SAS</publisher><subject>Animals ; chromosome ; Chromosomes - chemistry ; Chromosomes - ultrastructure ; dinoflagellate ; Dinoflagellida - chemistry ; Dinoflagellida - genetics ; Dinoflagellida - ultrastructure ; DNA, Protozoan - analysis ; Fluorescent Antibody Technique ; immuno-histochemistry ; Lysine - analysis ; Marine ; Microscopy, Immunoelectron ; Molecular Weight ; nuclear protein ; Nuclear Proteins - analysis ; Nuclear Proteins - chemistry ; Oxyrrhis marina</subject><ispartof>Biology of the cell, 1997-03, Vol.89 (1), p.43-52</ispartof><rights>1997</rights><rights>1997 Société Française des Microscopies and Société Biologie Cellulaire de France</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5467-cc16d31f0a7511e20a13a2547c6ced0e394e8abf15229fdfda96e888abe0f33a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0248-4900%2899%2980080-X$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0248-4900%2899%2980080-X$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9297782$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kato, Koichi H</creatorcontrib><creatorcontrib>Moriyama, Akihiko</creatorcontrib><creatorcontrib>Huitorel, Philippe</creatorcontrib><creatorcontrib>Cosson, Jacky</creatorcontrib><creatorcontrib>Cachon, Monique</creatorcontrib><creatorcontrib>Sato, Hidemi</creatorcontrib><title>Isolation of the major basic nuclear protein and its localization on chromosomes of the dinoflagellate, Oxyrrhis marina</title><title>Biology of the cell</title><addtitle>Biol Cell</addtitle><description>Basic nuclear proteins were extracted from isolated nuclei of
Oxyrrhis marina. The HPLC pattern of the extract showed a single major peak, which consisted of a major band with an apparent molecular mass of 23 kDa on an SDS-PAGE gel. We designated this protein as Np23 because of its apparent molecular mass. The amino acid composition of this protein revealed its extremely basic nature with a high lysine content. Polyclonal antibodies were raised against Np23. Immunofluorescence microscopy showed that Np23 was localized within the nucleus of dividing and non-dividing cells as well, and immuno-electron microscopy showed that the protein was localized only on chromosomes. These data established that Np23 is the major basic chromosome protein of
Oxyrrhis marina.</description><subject>Animals</subject><subject>chromosome</subject><subject>Chromosomes - chemistry</subject><subject>Chromosomes - ultrastructure</subject><subject>dinoflagellate</subject><subject>Dinoflagellida - chemistry</subject><subject>Dinoflagellida - genetics</subject><subject>Dinoflagellida - ultrastructure</subject><subject>DNA, Protozoan - analysis</subject><subject>Fluorescent Antibody Technique</subject><subject>immuno-histochemistry</subject><subject>Lysine - analysis</subject><subject>Marine</subject><subject>Microscopy, Immunoelectron</subject><subject>Molecular Weight</subject><subject>nuclear protein</subject><subject>Nuclear Proteins - analysis</subject><subject>Nuclear Proteins - chemistry</subject><subject>Oxyrrhis marina</subject><issn>0248-4900</issn><issn>1768-322X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1uEzEUhS0EKmnhESp5hUBiwPaM_9hUNCqlUiGLFIjYWI7nDnGZGRd7QhueHqcTsi0rS77fOfa5B6FjSt5QQsXbOWGVKipNyEutXylCFCkWj9CESqGKkrHFYzTZI0_RYUrXhJBKK36ADjTTUio2QbcXKbR28KHHocHDCnBnr0PES5u8w_3atWAjvolhAN9j29fYDwm3wdnW_9npeuxWMXQhhQ7SP5va96Fp7Q9osz28xrO7TYwrn7J_9L19hp40tk3wfHceoS8fzq6mH4vL2fnF9P1l4XglZOEcFXVJG2IlpxQYsbS0jFfSCQc1gVJXoOyyoZwx3dRNbbUApfIVkKYsbXmEXoy-OcKvNaTBdD657ad6COtkpGZSc8UfBKmgVPCKZJCPoIshpQiNuYk-h9oYSsy2GXPfjNmu3Wht7psxi6w73j2wXnZQ71W7KvL83Ti_9S1s_s_UnM6mVFcyi4tR7NMAd3uxjT-NkKXk5tvncyM-Xc2_06-nZp75k5GHvPvfHqJJzkOfd-ojuMHUwT8Q5y9cmcDZ</recordid><startdate>199703</startdate><enddate>199703</enddate><creator>Kato, Koichi H</creator><creator>Moriyama, Akihiko</creator><creator>Huitorel, Philippe</creator><creator>Cosson, Jacky</creator><creator>Cachon, Monique</creator><creator>Sato, Hidemi</creator><general>Elsevier SAS</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>199703</creationdate><title>Isolation of the major basic nuclear protein and its localization on chromosomes of the dinoflagellate, Oxyrrhis marina</title><author>Kato, Koichi H ; Moriyama, Akihiko ; Huitorel, Philippe ; Cosson, Jacky ; Cachon, Monique ; Sato, Hidemi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5467-cc16d31f0a7511e20a13a2547c6ced0e394e8abf15229fdfda96e888abe0f33a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>chromosome</topic><topic>Chromosomes - chemistry</topic><topic>Chromosomes - ultrastructure</topic><topic>dinoflagellate</topic><topic>Dinoflagellida - chemistry</topic><topic>Dinoflagellida - genetics</topic><topic>Dinoflagellida - ultrastructure</topic><topic>DNA, Protozoan - analysis</topic><topic>Fluorescent Antibody Technique</topic><topic>immuno-histochemistry</topic><topic>Lysine - analysis</topic><topic>Marine</topic><topic>Microscopy, Immunoelectron</topic><topic>Molecular Weight</topic><topic>nuclear protein</topic><topic>Nuclear Proteins - analysis</topic><topic>Nuclear Proteins - chemistry</topic><topic>Oxyrrhis marina</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kato, Koichi H</creatorcontrib><creatorcontrib>Moriyama, Akihiko</creatorcontrib><creatorcontrib>Huitorel, Philippe</creatorcontrib><creatorcontrib>Cosson, Jacky</creatorcontrib><creatorcontrib>Cachon, Monique</creatorcontrib><creatorcontrib>Sato, Hidemi</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kato, Koichi H</au><au>Moriyama, Akihiko</au><au>Huitorel, Philippe</au><au>Cosson, Jacky</au><au>Cachon, Monique</au><au>Sato, Hidemi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of the major basic nuclear protein and its localization on chromosomes of the dinoflagellate, Oxyrrhis marina</atitle><jtitle>Biology of the cell</jtitle><addtitle>Biol Cell</addtitle><date>1997-03</date><risdate>1997</risdate><volume>89</volume><issue>1</issue><spage>43</spage><epage>52</epage><pages>43-52</pages><issn>0248-4900</issn><eissn>1768-322X</eissn><abstract>Basic nuclear proteins were extracted from isolated nuclei of
Oxyrrhis marina. The HPLC pattern of the extract showed a single major peak, which consisted of a major band with an apparent molecular mass of 23 kDa on an SDS-PAGE gel. We designated this protein as Np23 because of its apparent molecular mass. The amino acid composition of this protein revealed its extremely basic nature with a high lysine content. Polyclonal antibodies were raised against Np23. Immunofluorescence microscopy showed that Np23 was localized within the nucleus of dividing and non-dividing cells as well, and immuno-electron microscopy showed that the protein was localized only on chromosomes. These data established that Np23 is the major basic chromosome protein of
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| ispartof | Biology of the cell, 1997-03, Vol.89 (1), p.43-52 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library; Alma/SFX Local Collection |
| subjects | Animals chromosome Chromosomes - chemistry Chromosomes - ultrastructure dinoflagellate Dinoflagellida - chemistry Dinoflagellida - genetics Dinoflagellida - ultrastructure DNA, Protozoan - analysis Fluorescent Antibody Technique immuno-histochemistry Lysine - analysis Marine Microscopy, Immunoelectron Molecular Weight nuclear protein Nuclear Proteins - analysis Nuclear Proteins - chemistry Oxyrrhis marina |
| title | Isolation of the major basic nuclear protein and its localization on chromosomes of the dinoflagellate, Oxyrrhis marina |
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