Faster and easier radiochemical purity testing for [ 125I]sodium iothalamate
A previous method for determination of the radiochemical purity (RCP) value of [ 125I]sodium iothalamate uses two paper strips and solvents (total developing time is ∼2.5 h). To simplify and shorten the RCP testing procedure, our laboratory has developed a single-strip chromatography method that not...
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| Veröffentlicht in: | Nuclear medicine and biology 1997-07, Vol.24 (5), p.479-483 |
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| creator | Bush, Curtis R. Coughlin, Joanne E. Herold, Thomas J. Hung, Joseph C. |
| description | A previous method for determination of the radiochemical purity (RCP) value of [
125I]sodium iothalamate uses two paper strips and solvents (total developing time is ∼2.5
h). To simplify and shorten the RCP testing procedure, our laboratory has developed a single-strip chromatography method that not only distributes free
125I and [
125I]sodium iothalamate to different relative front (R
f) locations, but is also faster and easier to perform. RCP of [
125I]sodium iothalamate was determined with the use of a 10-cm instant thin-layer chromatography strip impregnated with polysilicic acid gel (ITLC-SA) as the solid phase, and a mobile phase of 2-butanol:acetic acid:water (140:2.5:70, v/v). By using autoradiography and counting the strip segments in a gamma counter, our results indicated that free
125I migrated to
R
f
= 0.89–1.00 while the [
125I]sodium iothalamate moved to
R
f
= 0.44–0.67. The total developing time for the single-strip ITLC-SA system was ∼ 1
h. |
| doi_str_mv | 10.1016/S0969-8051(97)80018-2 |
| format | Article |
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125I]sodium iothalamate uses two paper strips and solvents (total developing time is ∼2.5
h). To simplify and shorten the RCP testing procedure, our laboratory has developed a single-strip chromatography method that not only distributes free
125I and [
125I]sodium iothalamate to different relative front (R
f) locations, but is also faster and easier to perform. RCP of [
125I]sodium iothalamate was determined with the use of a 10-cm instant thin-layer chromatography strip impregnated with polysilicic acid gel (ITLC-SA) as the solid phase, and a mobile phase of 2-butanol:acetic acid:water (140:2.5:70, v/v). By using autoradiography and counting the strip segments in a gamma counter, our results indicated that free
125I migrated to
R
f
= 0.89–1.00 while the [
125I]sodium iothalamate moved to
R
f
= 0.44–0.67. The total developing time for the single-strip ITLC-SA system was ∼ 1
h.</description><identifier>ISSN: 0969-8051</identifier><identifier>EISSN: 1872-9614</identifier><identifier>DOI: 10.1016/S0969-8051(97)80018-2</identifier><identifier>PMID: 9290086</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>[ 125I]Sodium iothalamate ; Autoradiography ; Biological and medical sciences ; Chromatography, Thin Layer ; Contrast Media - analysis ; Contrast media. Radiopharmaceuticals ; Instant thin-layer chromatography ; Iodine Radioisotopes - analysis ; Iothalamic Acid - analysis ; Medical sciences ; Pharmacology. Drug treatments ; Quality Control ; Radiochemical purity ; Relative front ; Sensitivity and Specificity</subject><ispartof>Nuclear medicine and biology, 1997-07, Vol.24 (5), p.479-483</ispartof><rights>1997</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c252t-6c005acd8de800a2088c4c8cead85688938ea577027b404f9a780c242d59b50d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0969-8051(97)80018-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2780161$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9290086$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bush, Curtis R.</creatorcontrib><creatorcontrib>Coughlin, Joanne E.</creatorcontrib><creatorcontrib>Herold, Thomas J.</creatorcontrib><creatorcontrib>Hung, Joseph C.</creatorcontrib><title>Faster and easier radiochemical purity testing for [ 125I]sodium iothalamate</title><title>Nuclear medicine and biology</title><addtitle>Nucl Med Biol</addtitle><description>A previous method for determination of the radiochemical purity (RCP) value of [
125I]sodium iothalamate uses two paper strips and solvents (total developing time is ∼2.5
h). To simplify and shorten the RCP testing procedure, our laboratory has developed a single-strip chromatography method that not only distributes free
125I and [
125I]sodium iothalamate to different relative front (R
f) locations, but is also faster and easier to perform. RCP of [
125I]sodium iothalamate was determined with the use of a 10-cm instant thin-layer chromatography strip impregnated with polysilicic acid gel (ITLC-SA) as the solid phase, and a mobile phase of 2-butanol:acetic acid:water (140:2.5:70, v/v). By using autoradiography and counting the strip segments in a gamma counter, our results indicated that free
125I migrated to
R
f
= 0.89–1.00 while the [
125I]sodium iothalamate moved to
R
f
= 0.44–0.67. The total developing time for the single-strip ITLC-SA system was ∼ 1
h.</description><subject>[ 125I]Sodium iothalamate</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Thin Layer</subject><subject>Contrast Media - analysis</subject><subject>Contrast media. Radiopharmaceuticals</subject><subject>Instant thin-layer chromatography</subject><subject>Iodine Radioisotopes - analysis</subject><subject>Iothalamic Acid - analysis</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Quality Control</subject><subject>Radiochemical purity</subject><subject>Relative front</subject><subject>Sensitivity and Specificity</subject><issn>0969-8051</issn><issn>1872-9614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkN9LHDEQx0Ox6Kn9E4R9EKkP207iZpM8FZGqBwd9aH0SCXPJrBfZH9dkV_C_b_SWe21eJjCfmfnyYeyMwzcOvP7-G0xtSg2SfzXqUgNwXYpPbMG1EqWpeXXAFnvkiB2n9JKZuuJwyA6NMAC6XrDVLaaRYoG9LwhTyN-IPgxuQ11w2BbbKYbxrRgpjaF_LpohFo8FF3L5lAYfpq4Iw7jBFjsc6ZR9brBN9GWuJ-zh9uefm_ty9etueXO9Kp2QYixrByDRee0px0YBWrvKaUfotay1NleaUCoFQq0rqBqDSoMTlfDSrCX4qxN2sdu7jcPfKSezXUiO2hZ7GqZklRFK55dBuQNdHFKK1NhtDB3GN8vBvlu0HxbtuyJrlP2waEWeO5sPTOuO_H5q1pb753MfU5bUROxdSHssX8-7ecZ-7DDKMl6zW5tcoN6RD5HcaP0Q_hPkH4WNjYY</recordid><startdate>199707</startdate><enddate>199707</enddate><creator>Bush, Curtis R.</creator><creator>Coughlin, Joanne E.</creator><creator>Herold, Thomas J.</creator><creator>Hung, Joseph C.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199707</creationdate><title>Faster and easier radiochemical purity testing for [ 125I]sodium iothalamate</title><author>Bush, Curtis R. ; Coughlin, Joanne E. ; Herold, Thomas J. ; Hung, Joseph C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c252t-6c005acd8de800a2088c4c8cead85688938ea577027b404f9a780c242d59b50d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>[ 125I]Sodium iothalamate</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Thin Layer</topic><topic>Contrast Media - analysis</topic><topic>Contrast media. Radiopharmaceuticals</topic><topic>Instant thin-layer chromatography</topic><topic>Iodine Radioisotopes - analysis</topic><topic>Iothalamic Acid - analysis</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Quality Control</topic><topic>Radiochemical purity</topic><topic>Relative front</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bush, Curtis R.</creatorcontrib><creatorcontrib>Coughlin, Joanne E.</creatorcontrib><creatorcontrib>Herold, Thomas J.</creatorcontrib><creatorcontrib>Hung, Joseph C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nuclear medicine and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bush, Curtis R.</au><au>Coughlin, Joanne E.</au><au>Herold, Thomas J.</au><au>Hung, Joseph C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Faster and easier radiochemical purity testing for [ 125I]sodium iothalamate</atitle><jtitle>Nuclear medicine and biology</jtitle><addtitle>Nucl Med Biol</addtitle><date>1997-07</date><risdate>1997</risdate><volume>24</volume><issue>5</issue><spage>479</spage><epage>483</epage><pages>479-483</pages><issn>0969-8051</issn><eissn>1872-9614</eissn><abstract>A previous method for determination of the radiochemical purity (RCP) value of [
125I]sodium iothalamate uses two paper strips and solvents (total developing time is ∼2.5
h). To simplify and shorten the RCP testing procedure, our laboratory has developed a single-strip chromatography method that not only distributes free
125I and [
125I]sodium iothalamate to different relative front (R
f) locations, but is also faster and easier to perform. RCP of [
125I]sodium iothalamate was determined with the use of a 10-cm instant thin-layer chromatography strip impregnated with polysilicic acid gel (ITLC-SA) as the solid phase, and a mobile phase of 2-butanol:acetic acid:water (140:2.5:70, v/v). By using autoradiography and counting the strip segments in a gamma counter, our results indicated that free
125I migrated to
R
f
= 0.89–1.00 while the [
125I]sodium iothalamate moved to
R
f
= 0.44–0.67. The total developing time for the single-strip ITLC-SA system was ∼ 1
h.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>9290086</pmid><doi>10.1016/S0969-8051(97)80018-2</doi><tpages>5</tpages></addata></record> |
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| ispartof | Nuclear medicine and biology, 1997-07, Vol.24 (5), p.479-483 |
| issn | 0969-8051 1872-9614 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | [ 125I]Sodium iothalamate Autoradiography Biological and medical sciences Chromatography, Thin Layer Contrast Media - analysis Contrast media. Radiopharmaceuticals Instant thin-layer chromatography Iodine Radioisotopes - analysis Iothalamic Acid - analysis Medical sciences Pharmacology. Drug treatments Quality Control Radiochemical purity Relative front Sensitivity and Specificity |
| title | Faster and easier radiochemical purity testing for [ 125I]sodium iothalamate |
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