Isolation and characterization of the promoter and flanking regions of the gene encoding the human protein-synthesis-initiation factor 2α
The promoter region of the gene ( eIF-2α) for eukaryotic initiation factor 2α ( eIF-2α) was isolated from a human genomic library and its structure was determined by restriction mapping and nucleotide (nt) sequence analysis. The promoter region and twelve in vivo transcriptional start points ( tsp)...
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| Veröffentlicht in: | Gene 1989-09, Vol.81 (2), p.315-324 |
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| creator | Hümbelin, Markus Safer, Brian Chiorini, John A. Hershey, John W.B. Cohen, Roger B. |
| description | The promoter region of the gene (
eIF-2α) for eukaryotic initiation factor 2α (
eIF-2α) was isolated from a human genomic library and its structure was determined by restriction mapping and nucleotide (nt) sequence analysis. The promoter region and twelve in vivo transcriptional start points (
tsp) have been identified by endonuclease S1 mapping and their location confirmed by primer-extension analysis, using RNA isolated from human cells. The untranslated leader is 102 to 140 nt long depending upon the
tsp, and the 5' region of the mRNA has the potential for forming stable stem-loop structures. The nt sequence of the regions upstream and downstream from the
tsp contains neither a ‘TATA box’ nor a ‘CAAT box’, but does contain several direct and inverted repeats, as well as palindromic sequences near the
tsp. In addition, multiple consensus binding sites for a wide variety of regulatory proteins are present throughout upstream and downstream
tsp-flanking regions. |
| doi_str_mv | 10.1016/0378-1119(89)90192-3 |
| format | Article |
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eIF-2α) for eukaryotic initiation factor 2α (
eIF-2α) was isolated from a human genomic library and its structure was determined by restriction mapping and nucleotide (nt) sequence analysis. The promoter region and twelve in vivo transcriptional start points (
tsp) have been identified by endonuclease S1 mapping and their location confirmed by primer-extension analysis, using RNA isolated from human cells. The untranslated leader is 102 to 140 nt long depending upon the
tsp, and the 5' region of the mRNA has the potential for forming stable stem-loop structures. The nt sequence of the regions upstream and downstream from the
tsp contains neither a ‘TATA box’ nor a ‘CAAT box’, but does contain several direct and inverted repeats, as well as palindromic sequences near the
tsp. In addition, multiple consensus binding sites for a wide variety of regulatory proteins are present throughout upstream and downstream
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eIF-2α) for eukaryotic initiation factor 2α (
eIF-2α) was isolated from a human genomic library and its structure was determined by restriction mapping and nucleotide (nt) sequence analysis. The promoter region and twelve in vivo transcriptional start points (
tsp) have been identified by endonuclease S1 mapping and their location confirmed by primer-extension analysis, using RNA isolated from human cells. The untranslated leader is 102 to 140 nt long depending upon the
tsp, and the 5' region of the mRNA has the potential for forming stable stem-loop structures. The nt sequence of the regions upstream and downstream from the
tsp contains neither a ‘TATA box’ nor a ‘CAAT box’, but does contain several direct and inverted repeats, as well as palindromic sequences near the
tsp. In addition, multiple consensus binding sites for a wide variety of regulatory proteins are present throughout upstream and downstream
tsp-flanking regions.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Cloning, Molecular</subject><subject>endonuclease S1 mapping</subject><subject>Eukaryotic Initiation Factor-2 - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes - genetics</subject><subject>Genes. Genome</subject><subject>Genomic Library</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>nucleotide sequence</subject><subject>primer-extension analysis</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Recombinant DNA</subject><subject>Restriction Mapping</subject><subject>RNA Caps</subject><subject>RNA structure</subject><subject>RNA, Messenger - genetics</subject><subject>Single-Strand Specific DNA and RNA Endonucleases</subject><subject>transcriptional start points</subject><subject>translational efficiency</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1DAUhi1EVYbCG4CUBUKwCPiS-LJBQhWXSpW6gbXl2MczhsQudgapPAJvw4vwTHWaYZbUG0v_-c71R-gZwW8IJvwtZkK2hBD1SqrXChNFW_YAbYgUqsWYyYdoc0QeocelfMP19T09RadUYq6I2qDfFyWNZg4pNia6xu5MNnaGHH6tYvLNvIPmOqcpVfkO8qOJ30PcNhm2lSn_oC1EaCDa5Jbgouz2k4lL8gwhtuUmVrGE0oYY5rA28LVdyg39--cJOvFmLPD08J-hrx8_fDn_3F5efbo4f3_Z2o6IuRUcuMHGSEepUwpYD90gBo8lENlbzhlzmDDpKQdHO-M8HoAZ1dWj9d1A2Bl6udatc_3YQ5n1FIqFsW4FaV-0UFRITvi9IOlZj0VPK9itoM2plAxeX-cwmXyjCdaLV3oxQi9GaKn0nVea1bTnh_r7YQJ3TDqYU-MvDnFTrBl9NtGGcsQE4x3ulu7vVgzq0X4GyLrYUG0AFzLYWbsU_j_HLUJBsuQ</recordid><startdate>19890930</startdate><enddate>19890930</enddate><creator>Hümbelin, Markus</creator><creator>Safer, Brian</creator><creator>Chiorini, John A.</creator><creator>Hershey, John W.B.</creator><creator>Cohen, Roger B.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19890930</creationdate><title>Isolation and characterization of the promoter and flanking regions of the gene encoding the human protein-synthesis-initiation factor 2α</title><author>Hümbelin, Markus ; Safer, Brian ; Chiorini, John A. ; Hershey, John W.B. ; Cohen, Roger B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-76e6a0aa8d22d99e35e4b7bf08e185c6633d0138f26ed24adf0be3a9410154b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Cloning, Molecular</topic><topic>endonuclease S1 mapping</topic><topic>Eukaryotic Initiation Factor-2 - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes - genetics</topic><topic>Genes. Genome</topic><topic>Genomic Library</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>nucleotide sequence</topic><topic>primer-extension analysis</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Recombinant DNA</topic><topic>Restriction Mapping</topic><topic>RNA Caps</topic><topic>RNA structure</topic><topic>RNA, Messenger - genetics</topic><topic>Single-Strand Specific DNA and RNA Endonucleases</topic><topic>transcriptional start points</topic><topic>translational efficiency</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hümbelin, Markus</creatorcontrib><creatorcontrib>Safer, Brian</creatorcontrib><creatorcontrib>Chiorini, John A.</creatorcontrib><creatorcontrib>Hershey, John W.B.</creatorcontrib><creatorcontrib>Cohen, Roger B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hümbelin, Markus</au><au>Safer, Brian</au><au>Chiorini, John A.</au><au>Hershey, John W.B.</au><au>Cohen, Roger B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of the promoter and flanking regions of the gene encoding the human protein-synthesis-initiation factor 2α</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1989-09-30</date><risdate>1989</risdate><volume>81</volume><issue>2</issue><spage>315</spage><epage>324</epage><pages>315-324</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>The promoter region of the gene (
eIF-2α) for eukaryotic initiation factor 2α (
eIF-2α) was isolated from a human genomic library and its structure was determined by restriction mapping and nucleotide (nt) sequence analysis. The promoter region and twelve in vivo transcriptional start points (
tsp) have been identified by endonuclease S1 mapping and their location confirmed by primer-extension analysis, using RNA isolated from human cells. The untranslated leader is 102 to 140 nt long depending upon the
tsp, and the 5' region of the mRNA has the potential for forming stable stem-loop structures. The nt sequence of the regions upstream and downstream from the
tsp contains neither a ‘TATA box’ nor a ‘CAAT box’, but does contain several direct and inverted repeats, as well as palindromic sequences near the
tsp. In addition, multiple consensus binding sites for a wide variety of regulatory proteins are present throughout upstream and downstream
tsp-flanking regions.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2806919</pmid><doi>10.1016/0378-1119(89)90192-3</doi><tpages>10</tpages></addata></record> |
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| ispartof | Gene, 1989-09, Vol.81 (2), p.315-324 |
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| subjects | Base Sequence Biological and medical sciences Blotting, Southern Cloning, Molecular endonuclease S1 mapping Eukaryotic Initiation Factor-2 - genetics Fundamental and applied biological sciences. Psychology Genes - genetics Genes. Genome Genomic Library Humans Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Conformation nucleotide sequence primer-extension analysis Promoter Regions, Genetic - genetics Recombinant DNA Restriction Mapping RNA Caps RNA structure RNA, Messenger - genetics Single-Strand Specific DNA and RNA Endonucleases transcriptional start points translational efficiency |
| title | Isolation and characterization of the promoter and flanking regions of the gene encoding the human protein-synthesis-initiation factor 2α |
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