Incorporation of cells into an ELISA system enhances antigen-driven lymphokine detection

The ability to measure successfully the levels of Th 1 or Th 2 cytokines during an in vitro antigen-driven, polyclonal T-cell response has proven to be more difficult than expected. Here we describe the development of a highly sensitive cell-based ELISA (celELISA) technique for the detection of muri...

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Veröffentlicht in:	Journal of immunological methods 1997-07, Vol.205 (2), p.163-168
Hauptverfasser:	Beech, Jonathan T, Bainbridge, Tracy, Thompson, Stephen J
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Animals Antigens - administration & dosage Bacterial Proteins Cells, Cultured Chaperonin 60 Chaperonins - administration & dosage Chaperonins - immunology Concanavalin A - administration & dosage Cytokine ELISA Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - statistics & numerical data Evaluation Studies as Topic Immunization Interferon-gamma - analysis Interferon-gamma - biosynthesis Interleukin-2 - analysis Interleukin-2 - biosynthesis Interleukin-4 - analysis Interleukin-4 - biosynthesis Lymphocyte Activation Lymphokines - analysis Lymphokines - biosynthesis Male Mice Mice, Inbred CBA Sensitivity and Specificity Spleen - cytology Spleen - immunology Th1 Cells - immunology Th2 Cells - immunology
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