Incorporation of cells into an ELISA system enhances antigen-driven lymphokine detection
The ability to measure successfully the levels of Th 1 or Th 2 cytokines during an in vitro antigen-driven, polyclonal T-cell response has proven to be more difficult than expected. Here we describe the development of a highly sensitive cell-based ELISA (celELISA) technique for the detection of muri...
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| Veröffentlicht in: | Journal of immunological methods 1997-07, Vol.205 (2), p.163-168 |
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| container_end_page | 168 |
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| container_issue | 2 |
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| container_title | Journal of immunological methods |
| container_volume | 205 |
| creator | Beech, Jonathan T Bainbridge, Tracy Thompson, Stephen J |
| description | The ability to measure successfully the levels of Th
1 or Th
2 cytokines during an in vitro antigen-driven, polyclonal T-cell response has proven to be more difficult than expected. Here we describe the development of a highly sensitive cell-based ELISA (celELISA) technique for the detection of murine Th
1 and Th
2 cytokines. The celELISA combines the quantification aspects of the conventional sandwich ELISA with the sensitivity of the ELISPOT assay. The celELISA was particularly useful for the improved detection of IL-2, IL-4, and to a lessor extent, IFN-γ. |
| doi_str_mv | 10.1016/S0022-1759(97)00072-0 |
| format | Article |
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| identifier | ISSN: 0022-1759 |
| ispartof | Journal of immunological methods, 1997-07, Vol.205 (2), p.163-168 |
| issn | 0022-1759 1872-7905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79274899 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | AIDS/HIV Animals Antigens - administration & dosage Bacterial Proteins Cells, Cultured Chaperonin 60 Chaperonins - administration & dosage Chaperonins - immunology Concanavalin A - administration & dosage Cytokine ELISA Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - statistics & numerical data Evaluation Studies as Topic Immunization Interferon-gamma - analysis Interferon-gamma - biosynthesis Interleukin-2 - analysis Interleukin-2 - biosynthesis Interleukin-4 - analysis Interleukin-4 - biosynthesis Lymphocyte Activation Lymphokines - analysis Lymphokines - biosynthesis Male Mice Mice, Inbred CBA Sensitivity and Specificity Spleen - cytology Spleen - immunology Th1 Cells - immunology Th2 Cells - immunology |
| title | Incorporation of cells into an ELISA system enhances antigen-driven lymphokine detection |
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