Enzyme-linked lectinsorbent assay measures N-acetyl-D-glucosamine in matrix of biofilm produced by Staphylococcus epidermidis

An enzyme-linked lectinsorbent assay (ELLA) was developed for quantification of in situ biofilm produced by Staphylococcus epidermidis in polystyrene 96-well tissue culture plates with phosphatase-labeled wheat germ agglutinin (WGA) as a specific probe for the GlcNAcbeta-1,4n component of exocellula...

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Veröffentlicht in:	Current microbiology 1997-10, Vol.35 (4), p.249-254
Hauptverfasser:	THOMAS, V. L, SANFORD, B. A, MORENO, R, RAMSAY, M. A
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylglucosamine - analysis Acetylglucosamine - biosynthesis Alkaline Phosphatase Bacteriological methods and techniques used in bacteriology Bacteriology Biofilms Biological and medical sciences Biotechnology Enzymes Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Humans Lectins Methods Microbiology Reproducibility of Results Staphylococcus epidermidis - isolation & purification Staphylococcus epidermidis - metabolism Wheat Germ Agglutinins
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