Cross-reactivity of an antiserum to the α-subunit of the Na+,K+-ATPase of toad (Bufo marinus) kidney with basal and apical membranes of transporting epithelia of the rat
An antibody to the 96 kD alpha-subunit of the Na+, K+-ATPase from Bufo marinus has been used in immunostaining rat kidney and salivary glands. Intense staining was observed on basolateral membranes of distal tubules of the kidney and striated ducts of the three major salivary glands. Less intense st...
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| Veröffentlicht in: | Cell and tissue research 1989-10, Vol.258 (1), p.137-145 |
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| creator | GRAVES, J. S INABNETT, T GEERING, K SIMSON, J. A. V |
| description | An antibody to the 96 kD alpha-subunit of the Na+, K+-ATPase from Bufo marinus has been used in immunostaining rat kidney and salivary glands. Intense staining was observed on basolateral membranes of distal tubules of the kidney and striated ducts of the three major salivary glands. Less intense staining was seen on the basolateral membranes of parotid acinar cells, but no staining was seen on the acinar cells of submandibular or sublingual glands. These sites of staining have been shown, by other methods, to posses substantial Na+, K+-ATPase, indicating that the antibody recognizes antigenic determinants of the sodium pump highly conserved in the course of evolution. In addition, staining with this antibody was observed at the apical region of cells of the proximal straight tubule and of the papillary collecting duct in the kidney. Absorption studies suggest that the apical antigenic determinants are the same or closely related to each other but are distinct from basolateral antigenic determinants. |
| doi_str_mv | 10.1007/BF00223153 |
| format | Article |
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S ; INABNETT, T ; GEERING, K ; SIMSON, J. A. V</creator><creatorcontrib>GRAVES, J. S ; INABNETT, T ; GEERING, K ; SIMSON, J. A. V</creatorcontrib><description>An antibody to the 96 kD alpha-subunit of the Na+, K+-ATPase from Bufo marinus has been used in immunostaining rat kidney and salivary glands. Intense staining was observed on basolateral membranes of distal tubules of the kidney and striated ducts of the three major salivary glands. Less intense staining was seen on the basolateral membranes of parotid acinar cells, but no staining was seen on the acinar cells of submandibular or sublingual glands. These sites of staining have been shown, by other methods, to posses substantial Na+, K+-ATPase, indicating that the antibody recognizes antigenic determinants of the sodium pump highly conserved in the course of evolution. In addition, staining with this antibody was observed at the apical region of cells of the proximal straight tubule and of the papillary collecting duct in the kidney. Absorption studies suggest that the apical antigenic determinants are the same or closely related to each other but are distinct from basolateral antigenic determinants.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/BF00223153</identifier><identifier>PMID: 2478290</identifier><identifier>CODEN: CTSRCS</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Basement Membrane - enzymology ; Biological and medical sciences ; Biological Transport ; Bufo marinus ; Cross Reactions ; Enzymes and enzyme inhibitors ; Epithelium - enzymology ; Epitopes - analysis ; Female ; Fundamental and applied biological sciences. Psychology ; Hydrolases ; Immunoenzyme Techniques ; Immunosorbent Techniques ; Kidney - enzymology ; Rats ; Rats, Inbred Strains ; Salivary Glands - enzymology ; Sodium - metabolism ; Sodium-Potassium-Exchanging ATPase - analysis ; Sodium-Potassium-Exchanging ATPase - immunology</subject><ispartof>Cell and tissue research, 1989-10, Vol.258 (1), p.137-145</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6706193$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2478290$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GRAVES, J. S</creatorcontrib><creatorcontrib>INABNETT, T</creatorcontrib><creatorcontrib>GEERING, K</creatorcontrib><creatorcontrib>SIMSON, J. A. V</creatorcontrib><title>Cross-reactivity of an antiserum to the α-subunit of the Na+,K+-ATPase of toad (Bufo marinus) kidney with basal and apical membranes of transporting epithelia of the rat</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><description>An antibody to the 96 kD alpha-subunit of the Na+, K+-ATPase from Bufo marinus has been used in immunostaining rat kidney and salivary glands. Intense staining was observed on basolateral membranes of distal tubules of the kidney and striated ducts of the three major salivary glands. Less intense staining was seen on the basolateral membranes of parotid acinar cells, but no staining was seen on the acinar cells of submandibular or sublingual glands. These sites of staining have been shown, by other methods, to posses substantial Na+, K+-ATPase, indicating that the antibody recognizes antigenic determinants of the sodium pump highly conserved in the course of evolution. In addition, staining with this antibody was observed at the apical region of cells of the proximal straight tubule and of the papillary collecting duct in the kidney. Absorption studies suggest that the apical antigenic determinants are the same or closely related to each other but are distinct from basolateral antigenic determinants.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Basement Membrane - enzymology</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Bufo marinus</subject><subject>Cross Reactions</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Epithelium - enzymology</subject><subject>Epitopes - analysis</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolases</subject><subject>Immunoenzyme Techniques</subject><subject>Immunosorbent Techniques</subject><subject>Kidney - enzymology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Salivary Glands - enzymology</subject><subject>Sodium - metabolism</subject><subject>Sodium-Potassium-Exchanging ATPase - analysis</subject><subject>Sodium-Potassium-Exchanging ATPase - immunology</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UMtOHDEQtFAQLIQLdyQfoihoGeLX2jNHWPESK8iBSLmtesae4GReuD2g_aXc-JF8UwxskFrqVnV1dVcTss_ZMWfMfD09Z0wIyWdyg0y4kiJjuck_kAmTTGRG6x_bZAfxF2NcaV1skS2hTC4KNiF_5qFHzIKDKvpHH1e0ryl0KaJHF8aWxp7Ge0f_Pmc4lmPn4wvjBbmB6dH1NDu5-wboXsEeLP1yOtY9bSH4bsRD-tvbzq3ok4_3tASEJilbCoOvUtm6tgzQOXydThUOfYi--0ndkAZc4-H_sgDxI9msoUG3t8675Pv52d38MlvcXlzNTxbZIOQsZiBB1LVWWhmwTgiWrAKUwjIl6yKBXPIqN6zUOVNWGatmRisBgttcFUrIXfL5TXcI_cPoMC5bj5VrmnRpP-LSFMKIIp8l4sGaOJats8sh-OR7tVw_N_U_rfuAyW6dDFYe32naMM0LKf8BaraImQ</recordid><startdate>19891001</startdate><enddate>19891001</enddate><creator>GRAVES, J. 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Psychology</topic><topic>Hydrolases</topic><topic>Immunoenzyme Techniques</topic><topic>Immunosorbent Techniques</topic><topic>Kidney - enzymology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Salivary Glands - enzymology</topic><topic>Sodium - metabolism</topic><topic>Sodium-Potassium-Exchanging ATPase - analysis</topic><topic>Sodium-Potassium-Exchanging ATPase - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GRAVES, J. S</creatorcontrib><creatorcontrib>INABNETT, T</creatorcontrib><creatorcontrib>GEERING, K</creatorcontrib><creatorcontrib>SIMSON, J. A. V</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GRAVES, J. S</au><au>INABNETT, T</au><au>GEERING, K</au><au>SIMSON, J. A. V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cross-reactivity of an antiserum to the α-subunit of the Na+,K+-ATPase of toad (Bufo marinus) kidney with basal and apical membranes of transporting epithelia of the rat</atitle><jtitle>Cell and tissue research</jtitle><addtitle>Cell Tissue Res</addtitle><date>1989-10-01</date><risdate>1989</risdate><volume>258</volume><issue>1</issue><spage>137</spage><epage>145</epage><pages>137-145</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><coden>CTSRCS</coden><abstract>An antibody to the 96 kD alpha-subunit of the Na+, K+-ATPase from Bufo marinus has been used in immunostaining rat kidney and salivary glands. Intense staining was observed on basolateral membranes of distal tubules of the kidney and striated ducts of the three major salivary glands. Less intense staining was seen on the basolateral membranes of parotid acinar cells, but no staining was seen on the acinar cells of submandibular or sublingual glands. These sites of staining have been shown, by other methods, to posses substantial Na+, K+-ATPase, indicating that the antibody recognizes antigenic determinants of the sodium pump highly conserved in the course of evolution. In addition, staining with this antibody was observed at the apical region of cells of the proximal straight tubule and of the papillary collecting duct in the kidney. Absorption studies suggest that the apical antigenic determinants are the same or closely related to each other but are distinct from basolateral antigenic determinants.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>2478290</pmid><doi>10.1007/BF00223153</doi><tpages>9</tpages></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Animals Basement Membrane - enzymology Biological and medical sciences Biological Transport Bufo marinus Cross Reactions Enzymes and enzyme inhibitors Epithelium - enzymology Epitopes - analysis Female Fundamental and applied biological sciences. Psychology Hydrolases Immunoenzyme Techniques Immunosorbent Techniques Kidney - enzymology Rats Rats, Inbred Strains Salivary Glands - enzymology Sodium - metabolism Sodium-Potassium-Exchanging ATPase - analysis Sodium-Potassium-Exchanging ATPase - immunology |
| title | Cross-reactivity of an antiserum to the α-subunit of the Na+,K+-ATPase of toad (Bufo marinus) kidney with basal and apical membranes of transporting epithelia of the rat |
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