The expression and activity of prostaglandin h synthase-2 is enhanced in trophoblast from women with preeclampsia
Preeclampsia is associated with altered biosynthesis of vasoactive prostanoids in placental villi. The two isozymes of prostaglandin H synthase (PGHS) are essential for prostanoid synthesis. We tested the hypothesis that PGHS-2 expression is elevated in trophoblast from preeclamptic women, compared...
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| Veröffentlicht in: | The journal of clinical endocrinology and metabolism 1997-09, Vol.82 (9), p.3059-3062 |
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| container_title | The journal of clinical endocrinology and metabolism |
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| creator | JOHNSON, R. D SADOVSKY, Y GRAHAM, C ANTEBY, E. Y POLAKOSKI, K. L HUANG, X NELSON, D. M |
| description | Preeclampsia is associated with altered biosynthesis of vasoactive prostanoids in placental villi. The two isozymes of prostaglandin H synthase (PGHS) are essential for prostanoid synthesis. We tested the hypothesis that PGHS-2 expression is elevated in trophoblast from preeclamptic women, compared with trophoblast from healthy women. Using immunofluorescent staining, we demonstrated a higher PGHS-2 expression in villi from preeclampsia, compared with normal pregnancy. Cytotrophoblasts cultured from placentas of preeclamptic women expressed higher levels of PGHS-2 compared with cytotrophoblasts from normal placentas. This enhanced expression of PGHS-2 correlated with increased media levels of both thromboxane and prostaglandin E2, two products of PGHS activity. The increased prostanoid production by trophoblast from preeclamptic women was markedly reduced by NS-398, a specific inhibitor of PGHS-2. We conclude that both expression and activity of PGHS-2 are enhanced in trophoblasts from preeclamptic women compared with trophoblast from normal pregnancies. The increased production of prostanoids may contribute to the clinical syndrome of preeclampsia. Our data suggest that a selective inhibitor of PGHS-2 might provide a therapeutic alternative to prophylactic low-dose aspirin in modifying the prostanoid profile in preeclampsia. |
| doi_str_mv | 10.1210/jc.82.9.3059 |
| format | Article |
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D ; SADOVSKY, Y ; GRAHAM, C ; ANTEBY, E. Y ; POLAKOSKI, K. L ; HUANG, X ; NELSON, D. M</creator><creatorcontrib>JOHNSON, R. D ; SADOVSKY, Y ; GRAHAM, C ; ANTEBY, E. Y ; POLAKOSKI, K. L ; HUANG, X ; NELSON, D. M</creatorcontrib><description>Preeclampsia is associated with altered biosynthesis of vasoactive prostanoids in placental villi. The two isozymes of prostaglandin H synthase (PGHS) are essential for prostanoid synthesis. We tested the hypothesis that PGHS-2 expression is elevated in trophoblast from preeclamptic women, compared with trophoblast from healthy women. Using immunofluorescent staining, we demonstrated a higher PGHS-2 expression in villi from preeclampsia, compared with normal pregnancy. Cytotrophoblasts cultured from placentas of preeclamptic women expressed higher levels of PGHS-2 compared with cytotrophoblasts from normal placentas. This enhanced expression of PGHS-2 correlated with increased media levels of both thromboxane and prostaglandin E2, two products of PGHS activity. The increased prostanoid production by trophoblast from preeclamptic women was markedly reduced by NS-398, a specific inhibitor of PGHS-2. We conclude that both expression and activity of PGHS-2 are enhanced in trophoblasts from preeclamptic women compared with trophoblast from normal pregnancies. The increased production of prostanoids may contribute to the clinical syndrome of preeclampsia. Our data suggest that a selective inhibitor of PGHS-2 might provide a therapeutic alternative to prophylactic low-dose aspirin in modifying the prostanoid profile in preeclampsia.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jc.82.9.3059</identifier><identifier>PMID: 9284743</identifier><identifier>CODEN: JCEMAZ</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Biological and medical sciences ; Blotting, Northern ; Blotting, Western ; Cells, Cultured ; Cyclooxygenase Inhibitors - pharmacology ; Diseases of mother, fetus and pregnancy ; Female ; Fluorescent Antibody Technique ; Gynecology. Andrology. Obstetrics ; Humans ; Isoenzymes - antagonists & inhibitors ; Isoenzymes - genetics ; Isoenzymes - metabolism ; Medical sciences ; Nitrobenzenes - pharmacology ; Placenta - cytology ; Placenta - enzymology ; Pre-Eclampsia - enzymology ; Pregnancy ; Pregnancy. Fetus. Placenta ; Prostaglandin-Endoperoxide Synthases - adverse effects ; Prostaglandin-Endoperoxide Synthases - genetics ; Prostaglandin-Endoperoxide Synthases - metabolism ; Reference Values ; RNA, Messenger - metabolism ; Sulfonamides - pharmacology ; Trophoblasts - enzymology</subject><ispartof>The journal of clinical endocrinology and metabolism, 1997-09, Vol.82 (9), p.3059-3062</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-f864ed73cf03ca0e49fa30ef55108bd72dc11163e6456eec0567f7d3ef03e5233</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2803546$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9284743$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JOHNSON, R. D</creatorcontrib><creatorcontrib>SADOVSKY, Y</creatorcontrib><creatorcontrib>GRAHAM, C</creatorcontrib><creatorcontrib>ANTEBY, E. Y</creatorcontrib><creatorcontrib>POLAKOSKI, K. L</creatorcontrib><creatorcontrib>HUANG, X</creatorcontrib><creatorcontrib>NELSON, D. M</creatorcontrib><title>The expression and activity of prostaglandin h synthase-2 is enhanced in trophoblast from women with preeclampsia</title><title>The journal of clinical endocrinology and metabolism</title><addtitle>J Clin Endocrinol Metab</addtitle><description>Preeclampsia is associated with altered biosynthesis of vasoactive prostanoids in placental villi. The two isozymes of prostaglandin H synthase (PGHS) are essential for prostanoid synthesis. We tested the hypothesis that PGHS-2 expression is elevated in trophoblast from preeclamptic women, compared with trophoblast from healthy women. Using immunofluorescent staining, we demonstrated a higher PGHS-2 expression in villi from preeclampsia, compared with normal pregnancy. Cytotrophoblasts cultured from placentas of preeclamptic women expressed higher levels of PGHS-2 compared with cytotrophoblasts from normal placentas. This enhanced expression of PGHS-2 correlated with increased media levels of both thromboxane and prostaglandin E2, two products of PGHS activity. The increased prostanoid production by trophoblast from preeclamptic women was markedly reduced by NS-398, a specific inhibitor of PGHS-2. We conclude that both expression and activity of PGHS-2 are enhanced in trophoblasts from preeclamptic women compared with trophoblast from normal pregnancies. The increased production of prostanoids may contribute to the clinical syndrome of preeclampsia. Our data suggest that a selective inhibitor of PGHS-2 might provide a therapeutic alternative to prophylactic low-dose aspirin in modifying the prostanoid profile in preeclampsia.</description><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Blotting, Western</subject><subject>Cells, Cultured</subject><subject>Cyclooxygenase Inhibitors - pharmacology</subject><subject>Diseases of mother, fetus and pregnancy</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Isoenzymes - antagonists & inhibitors</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>Medical sciences</subject><subject>Nitrobenzenes - pharmacology</subject><subject>Placenta - cytology</subject><subject>Placenta - enzymology</subject><subject>Pre-Eclampsia - enzymology</subject><subject>Pregnancy</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>Prostaglandin-Endoperoxide Synthases - adverse effects</subject><subject>Prostaglandin-Endoperoxide Synthases - genetics</subject><subject>Prostaglandin-Endoperoxide Synthases - metabolism</subject><subject>Reference Values</subject><subject>RNA, Messenger - metabolism</subject><subject>Sulfonamides - pharmacology</subject><subject>Trophoblasts - enzymology</subject><issn>0021-972X</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtLHEEUhQsx6GiyyzZQC3FlT-rZ1bUUiQ8Q3BjIrqipvmXX0C_71sTMv0-Jg6sL93wcOB8h3zlbc8HZz21YN2Jt15Jpe0RW3CpdGW7NMVkxJnhljfhzSs4Qt4xxpbQ8ISdWNMoouSKvzx1Q-DcvgJimkfqxpT7k9DflPZ0inZcJs3_pyz-NtKO4H3PnESpBE1IYOz8GaGnJ8jLN3bTpPWYal2mgb9MAI31LuSstAKH3w4zJfyVfou8Rvh3uOfl9--v55r56fLp7uLl-rILkOlexqRW0RobIZPAMlI1eMohac9ZsWiPawDmvJdRK16Wd6dpE00ooPGgh5Tm5_OgtE153gNkNCQP0ZQpMO3TGitoaLgp49QGGshUXiG5e0uCXvePMvRt22-Aa4ax7N1zwH4fe3WaA9hM-KC35xSH3GHwfl2Io4ScmGia1quV_uX-FWw</recordid><startdate>19970901</startdate><enddate>19970901</enddate><creator>JOHNSON, R. D</creator><creator>SADOVSKY, Y</creator><creator>GRAHAM, C</creator><creator>ANTEBY, E. Y</creator><creator>POLAKOSKI, K. L</creator><creator>HUANG, X</creator><creator>NELSON, D. M</creator><general>Endocrine Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19970901</creationdate><title>The expression and activity of prostaglandin h synthase-2 is enhanced in trophoblast from women with preeclampsia</title><author>JOHNSON, R. D ; SADOVSKY, Y ; GRAHAM, C ; ANTEBY, E. Y ; POLAKOSKI, K. L ; HUANG, X ; NELSON, D. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-f864ed73cf03ca0e49fa30ef55108bd72dc11163e6456eec0567f7d3ef03e5233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Blotting, Western</topic><topic>Cells, Cultured</topic><topic>Cyclooxygenase Inhibitors - pharmacology</topic><topic>Diseases of mother, fetus and pregnancy</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Isoenzymes - antagonists & inhibitors</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - metabolism</topic><topic>Medical sciences</topic><topic>Nitrobenzenes - pharmacology</topic><topic>Placenta - cytology</topic><topic>Placenta - enzymology</topic><topic>Pre-Eclampsia - enzymology</topic><topic>Pregnancy</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>Prostaglandin-Endoperoxide Synthases - adverse effects</topic><topic>Prostaglandin-Endoperoxide Synthases - genetics</topic><topic>Prostaglandin-Endoperoxide Synthases - metabolism</topic><topic>Reference Values</topic><topic>RNA, Messenger - metabolism</topic><topic>Sulfonamides - pharmacology</topic><topic>Trophoblasts - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JOHNSON, R. D</creatorcontrib><creatorcontrib>SADOVSKY, Y</creatorcontrib><creatorcontrib>GRAHAM, C</creatorcontrib><creatorcontrib>ANTEBY, E. Y</creatorcontrib><creatorcontrib>POLAKOSKI, K. L</creatorcontrib><creatorcontrib>HUANG, X</creatorcontrib><creatorcontrib>NELSON, D. M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JOHNSON, R. D</au><au>SADOVSKY, Y</au><au>GRAHAM, C</au><au>ANTEBY, E. Y</au><au>POLAKOSKI, K. L</au><au>HUANG, X</au><au>NELSON, D. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The expression and activity of prostaglandin h synthase-2 is enhanced in trophoblast from women with preeclampsia</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>1997-09-01</date><risdate>1997</risdate><volume>82</volume><issue>9</issue><spage>3059</spage><epage>3062</epage><pages>3059-3062</pages><issn>0021-972X</issn><eissn>1945-7197</eissn><coden>JCEMAZ</coden><abstract>Preeclampsia is associated with altered biosynthesis of vasoactive prostanoids in placental villi. The two isozymes of prostaglandin H synthase (PGHS) are essential for prostanoid synthesis. We tested the hypothesis that PGHS-2 expression is elevated in trophoblast from preeclamptic women, compared with trophoblast from healthy women. Using immunofluorescent staining, we demonstrated a higher PGHS-2 expression in villi from preeclampsia, compared with normal pregnancy. Cytotrophoblasts cultured from placentas of preeclamptic women expressed higher levels of PGHS-2 compared with cytotrophoblasts from normal placentas. This enhanced expression of PGHS-2 correlated with increased media levels of both thromboxane and prostaglandin E2, two products of PGHS activity. The increased prostanoid production by trophoblast from preeclamptic women was markedly reduced by NS-398, a specific inhibitor of PGHS-2. We conclude that both expression and activity of PGHS-2 are enhanced in trophoblasts from preeclamptic women compared with trophoblast from normal pregnancies. The increased production of prostanoids may contribute to the clinical syndrome of preeclampsia. Our data suggest that a selective inhibitor of PGHS-2 might provide a therapeutic alternative to prophylactic low-dose aspirin in modifying the prostanoid profile in preeclampsia.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>9284743</pmid><doi>10.1210/jc.82.9.3059</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Biological and medical sciences Blotting, Northern Blotting, Western Cells, Cultured Cyclooxygenase Inhibitors - pharmacology Diseases of mother, fetus and pregnancy Female Fluorescent Antibody Technique Gynecology. Andrology. Obstetrics Humans Isoenzymes - antagonists & inhibitors Isoenzymes - genetics Isoenzymes - metabolism Medical sciences Nitrobenzenes - pharmacology Placenta - cytology Placenta - enzymology Pre-Eclampsia - enzymology Pregnancy Pregnancy. Fetus. Placenta Prostaglandin-Endoperoxide Synthases - adverse effects Prostaglandin-Endoperoxide Synthases - genetics Prostaglandin-Endoperoxide Synthases - metabolism Reference Values RNA, Messenger - metabolism Sulfonamides - pharmacology Trophoblasts - enzymology |
| title | The expression and activity of prostaglandin h synthase-2 is enhanced in trophoblast from women with preeclampsia |
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